Crisscross cooperative self-assembly

ABSTRACT

Provided herein, in some embodiments, are methods, compositions and kits for controlling nucleation and assembly of molecular nanostructures, microstructures and macrostructures.

RELATED APPLICATION

This application claims the benefit under 35 U.S.C. § 119(e) of U.S. provisional application No. 62/370,098, filed Aug. 2, 2016, which is incorporated by reference herein in its entirety.

FEDERALLY SPONSORED RESEARCH

This invention was made with government support under 1435964 awarded by the Office of Naval Research. The government has certain rights in the invention.

BACKGROUND

In nature, biomolecules assemble into hierarchical structures through intermolecular interactions. In synthetic biology, it is possible to rationally design biosynthetic building blocks, with hierarchical structures arising from built-in functionality at the molecular level controlling intermolecular interactions. Such biosynthetic self-assembling structures have useful applications in the field of nanotechnology, for example.

SUMMARY

Provided herein, in some embodiments, is a technology (including, for example, methods, compositions and kits) for controlling nucleation and hierarchical assembly (programmable self-assembly) of molecular structures, such as nucleic acid (e.g., DNA) and/or protein nanostructures, microstructures, and macrostructures. This technology, referred to herein as ‘crisscross cooperative assembly’ can be used to program and rapidly assemble structures that only originate from provided macromolecular ‘seeds,’ thus may be considered a ‘zero-background’ assembly method. Through the design of cooperative binding sites on individual biomolecular subunits that require simultaneous engagement with a large number of other subunits to achieve stable attachment, the system imposes an intrinsically high energetic barrier against spontaneous nucleation of structures, even in the presence of high concentrations of each individual component. Nucleation can only be triggered by providing a macromolecular ‘seed’ that resembles a pre-existing structural interface (presents multiple weak binding sites for stable capture of the next subunit). Addition of a seed that can stably capture individual subunits effectively bypasses the activation energy barrier against spontaneous nucleation to drive higher-order assembly of a microscale structure. Components can be continually added to the structures such that their growth in one-dimension, two-dimensions or three-dimensions is potentially as large as for other polymerization or crystallization processes.

Crisscross cooperative assembly, as provided herein, uses molecular (e.g., nucleic acid or protein) building blocks (FIG. 1A) that are programmed to self-assemble into crisscrossed layers (FIG. 1B). A building block, in some embodiments, may be a rod-shaped structure assembled from programmable nucleic acid hybridization interactions. As indicated above, this crisscross cooperative assembly technology uses a ‘seed’ structure from which programmable nucleic acid self-assembly begins. This seed structure is formed through irreversible interactions between a nucleating structure (FIG. 1A; ‘queen’) and a subset of building blocks (FIG. 1A; ‘drones’) that are aligned to form an initial seed layer along the nucleating structure. In the presence of a seed structure, another set of building blocks (FIG. 1A; ‘workers’) are added to the pre-existing seed layer (FIG. 1B). Binding between a sufficient number of building blocks (drones) and a nucleating structure (queen) to form a seed can trigger the addition of many additional layers of building blocks (workers), with each layer rotated by some degree (e.g., 90°) relative to adjacent layers (above and/or below).

The nucleating structure and the building blocks are engineered to interact with (e.g., bind to) each other based on a set of kinetic/nucleation energy parameters, as follows. An initial subset of building blocks (drones) should bind strongly (irreversibly/stably) to and form an aligned layer along the nucleating structure (queen). The building blocks (drones) of the initial subset should not interact with (bind to) each other. Likewise, building blocks (workers) of a subsequent subset should not interact with (bind to) each other. Further, in the absence of a nucleating structure (queen), any building block (drone) from the initial subset should have only one weak (reversible) interaction with any other building block (worker) from another subset. In the presence of a nucleating structure (queen), a single building block (drone) from an initial subset may interact with more than one building block (worker) from a subsequent subset, and a single building block (worker) from a subsequent subset may interact with more than one building block (drone) from the initial subset or another subset (‘workers’ of another subset). For example, with reference to FIG. 1B, a single building block (e.g., DNA nanorod) may bind to eight other building blocks (e.g., DNA nanorods), although the single building block binds to each of the eight building blocks only once to form two layers having a ‘crisscross’ pattern.

The single interaction between a building block (drone) from the initial subset and a building block from a subsequent subset (worker) should be weak enough such that there is an arbitrarily large entropy penalty against nucleation in the absence of a seed structure (a large number of individual workers would have to come together simultaneously). With these parameters, zero-background and minimal defects can be achieved, even at high concentrations of interacting building blocks, thereby enabling rapid nucleation and assembly of nucleic acid nanostructures.

Thus, provided herein are compositions, comprising (a) a nucleating nucleic acid nanostructure, (b) a first layer of parallel elongated nucleic acid nanostructures stably bound to the nucleating nanostructure of (a), and (c) a second layer of parallel elongated nucleic acid nanostructures stably bound to the elongated nanostructures of (b) and rotated at an angle relative to the parallel elongated nanostructures of (b), wherein a single elongated nanostructure of (b) binds to multiple elongated nanostructures of (c), each through a single cooperative binding site. In some embodiments, a single elongated nanostructure of (c) binds to multiple elongated nanostructures of (b), each through a single cooperative binding site.

Also provided herein, in some aspects, are compositions comprising: (a) nucleating nanostructures; (b) a first subset of elongated nanostructures, wherein less than 10% of the nanostructures of (b) bind to each other, and wherein the nanostructures of (b) irreversibly bind to a nucleating nanostructure of (a); and (c) a second subset of elongated nanostructures, wherein less than 10% of the nanostructures of (c) bind to each other, wherein, in the absence of a nucleating nanostructure, a nanostructure of (b) can reversibly binding to a nanostructure of (a) only at a single position on the nanostructure of (a), and wherein, in the absence of a nucleating nanostructure, a nanostructure of (a) can reversibly binding to a nanostructure of (b) only at a single position on the nanostructure of (b). See, e.g., FIGS. 1A-1B.

Also provided herein, in some embodiments, are crisscross nucleic acid nanostructures, comprising a first nanorod comprising a first plug strand and a second plug strand; a second nanorod comprising a third plug strand and a fourth plug strand, wherein the second nanorod is parallel to the first nanorod; a third nanorod comprising a fifth plug strand complementary to and bound to the first plug strand and a sixth plug strand complementary to and bound to the second plug strand; a fourth nanorod comprising a seventh plug strand complementary to and bound to the third plug strand and an eighth plug strand complementary to and bound to the fourth plug strand, wherein the third nanorod is parallel to the fourth nanorod. See, e.g., FIG. 18. A crisscross nanostructure is not limited to 4 nanorods and, in many embodiments, includes at least 4 (e.g., at least 5, 10, 15, 20, 25, 50, 100 or more) nanorods arranged in a crisscross pattern as described herein.

Thus, in some embodiments, a crisscross nucleic acid nanostructure, comprises a first plurality of nanorods parallel to each other, and a second plurality of nanorods parallel to each other, wherein the nanorods of the first plurality are bound to and perpendicular to (or are non-parallel to) the nanorods of the second plurality. See, e.g., FIG. 18.

In some embodiments, each nanorod is comprised of DNA. For example, a nanorod may be comprised of a 6-helix DNA bundle (see, e.g., Douglas S M1, Chou J J, Shih W M. DNA-nanotube-induced alignment of membrane proteins for NMR structure determination. Proc Natl Acad Sci USA. 104, 6644-6648, 2007, incorporated herein by reference).

Also provided herein, in some aspects, are crisscross nucleic acid slats, comprising: a first plurality of at least four nucleic acid strands parallel to each other, each strand of the first plurality having a length of 20-100 nucleotides (e.g., 20-30, 20-40 or 20-50 nucleotides); and a second plurality of at least four nucleic acid strands parallel to each, each strand of the second plurality having a length of 20-100 nucleotides (e.g., 20-30, 20-40 or 20-50 nucleotides), wherein the at least four nucleic acid strands of the first plurality are bound to and perpendicular to the at least four nucleic acid strands of the second plurality. See, e.g., FIGS. 21A-21B.

Also provided herein, in some aspects are crisscross nucleic acid slats, comprising: a first plurality of at least four nucleic acid strands parallel to each other, each strand of the first plurality having a length of at least 21 nucleotides; and a second plurality of at least four nucleic acid strands parallel to each, each strand of the second plurality having a length of at least 21 nucleotides, wherein the at least four nucleic acid strands of the first plurality are bound to and perpendicular to the at least four nucleic acid strands of the second plurality. See, e.g., FIGS. 21A-21B.

Further provided herein, in some aspects, are nucleic acid nanostructures comprising a nucleic acid scaffold strand folded (e.g., M13 or M13-derived) into repeating loop-like shapes (e.g., 5-15 loops, or 5, 6, 7, 8, 9 or 10 loops) secured by shorter nucleic acid staple strands, wherein the repeating loop structures are bound to at least one (e.g., at least 2, 3, 4, 5, 10, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or more) crisscross nucleic acid slat. See, e.g., FIGS. 22, 24A, 25 and 27B.

Further still, provided herein, in some aspects, are nucleic acid nanostructures, comprising a nucleic acid scaffold strand folded into repeating loop-like shapes secured by at least two crisscross nucleic acid slats. See, e.g., FIGS. 27B and 28B.

The present disclosure also provides, in some aspects, methods of producing a crisscross nucleic acid nanostructures, comprising: combining in a reaction mixture (a) a first nanorod comprising a first plug strand and a second plug strand, (b) a second nanorod comprising a third plug strand and a fourth plug strand, wherein the second nanorod is parallel to the first nanorod, (c) a third nanorod comprising a fifth plug strand complementary to and bound to the first plug strand and a sixth plug strand complementary to and bound to the second plug strand, and (d) a fourth nanorod comprising a seventh plug strand complementary to and bound to the third plug strand and an eighth plug strand complementary to and bound to the fourth plug strand, wherein the third nanorod is parallel to the fourth nanorod; and incubating the reaction mixture under conditions (e.g., nucleic acid hybridization conditions) that result in assembly of a crisscross nucleic acid nanostructure. See, e.g., FIG. 22.

Biomolecule (analyte) detection methods are also provided herein, in some aspects. In some embodiments, a method, comprises (a) combining in a reaction mixture (i) a sample comprising a biomolecule; (ii) a nucleic acid strand capable of self-assembling into a nanostructure that comprise vertically-stacked parallel strands; (iii) a plurality of oligonucleotides, shorter than the nucleic acid strand of (ii), wherein the oligonucleotides of (iii) bind to the strand of (ii) to assemble the vertically-stacked parallel strands; (iv) two crisscross nucleic acid slats, wherein the two slats bind to the strand of (ii), and wherein each of the slats is linked to a biomolecule binding partner that specifically binds to the biomolecule in the sample; (b) incubating the reaction mixture under conditions that permit binding of the biomolecule binding partners to the biomolecule and assembly of the nanostructure into vertically-stacked parallel strands; (c) removing the plurality of oligonucleotides of (iii) from the reaction mixture of (b); (d) incubating the reaction mixture of (c) in the presence of a plurality of crisscross nucleic acid slats of claim 27, wherein the crisscross nucleic acid slats bind to the vertically-stacked parallel strands to form a three-dimensional barrel structure. In some embodiments, the methods further comprise imaging the three-dimensional barrel structure. See, e.g., FIGS. 28A-28B.

In some embodiments, the methods may comprise combining in a reaction mixture (e.g., with hybridization buffer) (a) a sample comprising a biomolecule and (b) a nucleic acid nanostructure comprising (i) a nucleic acid scaffold strand capable of folding into repeating loop-like shapes (e.g., 2-15 vertically-stacked loops) and (ii) two crisscross nucleic acid slats, wherein a biomolecule binding partner (e.g., an antibody) that specifically binds to the biomolecule is linked to each of the crisscross nucleic acid slats such that in the presence of the cognate biomolecule the biomolecule binding partner binds to the biomolecule and the nucleic acid nanostructure folds into repeating loop-like shapes. See, e.g., FIGS. 28A-28B.

In some embodiments, the methods further comprise combining the reaction mixture with a plurality (e.g., 2-50 or 2-100) of crisscross nucleic acid slats to form a three-dimensional barrel-like structure. See, e.g., FIG. 24B.

It should be understood that the nucleic acid nanostructures as described herein (e.g., nanorods, slats, barrels, etc.) and variants thereof, as provided herein, may be designed, for example, using the following publicly-available tool described by Douglas S M, Marblestone A H, Teerapittayanon S, Vazquez A, Church G M, Shih W M. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001-5006, 2009, incorporated herein by reference. See, also, Douglas et al. Nature, 459(7245): 414-418, 2009, incorporated herein by reference. For example, and as described elsewhere herein, it is known in the art that custom shape (e.g., megadalton-scale) DNA nanostructures may be produced using a long ‘scaffold’ strand to template the assembly of hundreds of oligonucleotide ‘staple’ strands into a planar antiparallel array of cross-linked helices. This ‘scaffolded DNA origami’ method has also been adapted to produce 3D shapes formed as pleated layers of double helices constrained to a honeycomb lattice. caDNAno, an open-source software package with a graphical user interface, may be used to aid in the design of DNA sequences for folding 3D DNA (or other nucleic acid) nanostructures. The caDNAno software is available at cadnano.org, along with example designs and video tutorials demonstrating their construction.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1B show an abstraction of an example of crisscross cooperative assembly system. FIG. 1A indicates the system without a nucleation site and no self-assembly. FIG. 1B indicates the system after the addition of the nucleation site and triggered spontaneous self-assembly. Growth direction is indicated by the grey arrows and shows a 1D growth in this example. Individual components are referred to as ‘queen,’ ‘drone’ and ‘worker.’

FIG. 2 shows a graph depicting the principle by which the nucleation site (queen) structure initiates higher-order structures with drones and workers by lowering the activation energy for assembly.

FIGS. 3A-3C show examples of DNA-origami crisscross assembly. FIG. 3A shows queen (Q) and drone/worker (D/W) architecture shown in cross-section (caDNAno software downloaded from cadnano.org) and in 3D representation. Each individual cylinder represents a double stranded DNA helix. FIG. 3B is a representation of 1D and 2D growth with the crisscross DNA-origami cooperative assembly. 3D growth can be achieved by creating a design that merges 1D and 2D growth. FIG. 3C shows different pathways for 2D growth.

FIG. 4 shows an example of single-stranded DNA crisscross cooperative assembly. Oligonucleotides comprising the workers and drones of the system (shown as cylinders) are nucleated by the addition of a cubic DNA-Origami queen structure with a nucleation site. Stepwise assembly is shown in illustrated magnification.

FIG. 5 shows an example of catenane crisscross cooperative assembly queen (catenane queen), useful for ultrasensitive detection. Oligonucleotides comprising the workers and drones of the system (shown as cylinders) are nucleated by the addition of a single-stranded catenane queen structure with a nucleation site. Binding sites on the structure shown to the left of the illustrated magnification indicate the nucleation for the workers/drones. Each host ring has multiple binding sites, collectively functioning as a cooperative binding site.

FIG. 6 shows a catenane queen from FIG. 5 that has been modified to serve as a biosensor. The large DNA ring has been split to incorporate and biomolecule capture site to bind a biomolecule (e.g., macromolecule) in biological samples. The presence of the biomolecule, in some embodiments may be detected in mixtures as follows: (1) A biological sample is mixed with a high concentration of the catenane queen and a biomolecule of interest binds the biomolecule capture site. (2) A chemical reaction is used to reversibly cleave the biomolecule capture site. (3) Catenane queens not bound to the target biomolecule fall apart more quickly compared to those held together by the target biomolecule. (4) Remaining catenane queens in the test mixture are re-ligated at the biomolecule capture site. (5) Drones and workers are added to the test mixture to amplify remaining queens using readily observable micrometer-scale DNA structures. This system is modular, as the biomolecule capture site may be customized to bind disease markers, including proteins or nucleic acid sequences.

FIG. 7 shows a CAD schematic of an example of base-pairing linkages between a 6 helix bundle worker and a 6 helix bundle drone to queen. A plug socket linkage design may also be used, as shown in FIGS. 18-20. The following CAD tool was used to design the structures: Douglas S M, Marblestone A H, Teerapittayanon S, Vazquez A, Church G M, Shih W M. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001-5006, 2009.

FIGS. 8A-8D are schematics depicting different example ‘seed’ designs (a 6-helix bundle nanorod bound to a nucleating nanostructure) with different cooperative bind site configurations. Additional example ‘seed’ designs are shown in FIGS. 16A-16C.

FIG. 9A-9B show results from a seesaw experiment with an example of a nucleating nanostructure (queen) folded at different temperatures (A: 65-60° C.; B: 60-55° C.; C: 65-55° C.; D: 60-50° C.) at a MgCl₂ concentration of 6 mM.

FIGS. 10A-10B show results from a seesaw experiment with an example of a nanostructure (drone) folded at different temperatures (A: 70-60° C.; B: 65-55° C.; C: 65-60° C.; D: 60-55° C.) at a MgCl₂ concentration of 6 mM.

FIG. 11 shows a schematic depicting the assembly of an example seed structure (queen+drones). Images of the structures are also shown. Nanostructure assembly may also be carried out as shown in FIGS. 19A-19D.

FIGS. 12A-12G show a seed structure forming from the assembly of a single-stranded DNA and additional nanostructures (drones).

FIGS. 13A-13B show results from seesaw experiments with a single-stranded nucleating nanostructure (queen) at various temperatures and steps.

FIGS. 14A-14C show results demonstrating that nanostructures (workers) assemble in the presence of a nucleating nanostructure (queen) but not in the absence of a nucleating nanostructure.

FIGS. 15A-15B show results an example of nanostructures not assembling in the absence of a nucleating nanostructure (queen).

FIG. 16A shows a two-dimensional view of the gridiron queen that can bind 16 drones simultaneously in a horizontal (coordinate x in FIG. 16B) across the queen. Staples necessary to fold the scaffold into the queen are shown, and the 3′ ends of the staples may be appended with overhanging single-stranded sequences to bind drones. A three-dimensional view (FIG. 16B) shows the queen with binding sites in each drone-docking cell. A transmission electron microscope (TEM) image of the queen is shown in FIG. 16C. Lateral dimensions of the structure are approximately 72 nm×240 nm.

FIGS. 17A-17B depict drone and worker subcomponents. FIG. 17A shows drone and worker subcomponents constructed from 6-helix bundle scaffolded DNA origami to form rods that are customizable in length. The 3′ ends of staples contain overhanging single strand DNA sequences that act as plug binding handles to interact with other components. Similarly, the lowermost helix contains socket sequences (i.e. single strand DNA scaffold not complemented by a folding staple) that accept plug sequences from other components. The plugs and socket respectively are periodic and can be situated, for example, every 42 bp (˜14 nm) along the length of the component. FIG. 17B shows TEM images of test drones folded from two different scaffold sequences. The drone in the top image is ˜250 nm in length, versus the drone in the bottom image, which is ˜440 nm in length.

FIG. 18 shows a detailed view of the plug-socket binding system. The case shown in the upper panel shows the full set of 5 single-stranded plug sequences extending from the queen (the small arrow) with matching socket sites in a six-helix bundle drone. In the lower pane, the binding sequence is drawn as a series of ‘X’ to indicate that both the length and sequence of the plug and socket may be varied. The scaffold sequences are drawn in black. Note that this design (shown for a drone-queen assembly) is also used to bind drones to workers. The gridiron queen sequences, from top to bottom, correspond to SEQ ID NOs: 685 and 686. The 6 helix bundle (hb) drone sequences, from left to right, correspond to SEQ ID NOs: 687 and 688.

FIGS. 19A-19D shows how the plug-socket binding system can be used to program drones to bind to desired sites on the queen. FIG. 19A shows two 440 nm drones placed in the middle two queen cells, FIG. 19B shows one 250 nm drone placed in the middle queen cell, and FIG. 19C shows 250 nm located in every cell of the queen. The desired design is shown to the left, versus a TEM image of the assembled structure to the right. FIG. 19D shows bulk analysis of the design from FIG. 19A using agarose gel electrophoresis for one design using a 7 bp plug-socket, and another with a 10 bp plug-socket.

FIG. 20 shows the extent of free queen remaining over time as it becomes bound to a single 440 nm drone.

FIGS. 21A-21B show an example of a crisscross DNA slat-based architecture. FIG. 21A is an abstraction of the crisscross DNA slats motif (right). Light and dark strands weave and are complementary to each other at each junction. The length of each binding site is shown on the right. Each row and column amount to 21 base pairs (bp). The matrix shows the number of base pairs (bp) per binding site at each position of the abstraction and 3D rendering. FIG. 21B is a 3D rendering of the DNA slats. On the left, the top down view shows the weaving of each strand. A cross section (A-A) is shown on the right.

FIG. 22 shows the steps to DNA slats assembly. Step 1 shows DNA-origami folding of an arbitrary DNA-origami queen (a barrel queen shown as an example). Step 2 is the mixing of the crude DNA-origami queen reaction (from step 1) with DNA slats at various salt concentrations, temperatures, and DNA slat concentration.

FIG. 23A shows a flat DNA-origami queen without any DNA slats added. FIG. 23B shows a flat DNA-origami queen with the addition of DNA slats and the correct formation of a sheet, by tiling the ssDNA scaffold of the queen with DNA slats. DNA slat tiled region is indicated in light gray. Scale bars on images are 600 nm and on enlarged view 100 nm.

FIG. 24A shows a barrel DNA-origami queen without any DNA slats added. Scale bar on image is 400 nm and on enlarged view 100 nm. FIG. 24B shows a barrel DNA-origami queen with the addition of DNA slats and the correct formation of a barrel, by tiling the ssDNA scaffold of the queen with DNA slats. Scale bar indicates 50 nm. DNA slat tiled region is indicated in light gray.

FIG. 25 depicts a growth mechanism of DNA slats seeded on a queen structure. The DNA-origami queen is mixed with DNA slats to tile the ssDNA scaffold of the queen and then later extend and polymerize the growth of micron-sized structures solely through DNA slats (DNA slats may be joined end-to-end, within the same plane, through nucleotide base pairing of adjacent slats). The upper design shows a flat DNA-origami queen with the growth of three linear sheets in the horizontal direction. The lower design shows a barrel DNA-origami queen with tubular growth in the vertical direction.

FIGS. 26A-26E show three extensions of the first generation of DNA slats binding to the flat DNA-origami queen. FIG. 26A shows first generation extensions tiled with a short second generation of DNA slats, resulting in three tooth-like extensions on the queen. FIG. 26B shows first generation extensions tiled with a long second generation of DNA slats, which are terminally tiled with short third generation DNA slats. FIGS. 26C-26E show first, second, and third generations of DNA slats which are complementary to one another, resulting in extensions of linear sheet structures. FIG. 26C contains one extended first generation, FIG. 26D contains two extended first generations, and FIG. 26E contains three extended first generations. Scale bars for FIGS. 26A-26C are 100 nm and for FIGS. 26D-26E, 20 0 nm.

FIGS. 27A-27E show formation of multi-host-ring catenane systems with DNA slats in a one-pot reaction. (FIG. 27A) Formation of eight loops with M13 scaffold through staple strands. Staple (“brown”) strands fold stable DNA-Origami base and DNA slats catenate the eight loops. (FIG. 27B) 3D view of barrel queen additionally serving as multi-host-ring catenane system with high yield typical for DNA-Origami. (FIG. 27C) Abstract and 3D view of DNA slats weaving through the ssDNA M13 scaffold loops on the barrel queen. Through ligation of one side a single DNA slat catenates all eight loops. (FIG. 27D) 3D rendering of DNA slat weaving and catenating eight separate ssDNA loops. Top shows a tilted bottom view and bottom a side view. (FIG. 27E) Former technique to achieve a maximum of four-host-ring catenane system with low yield.

FIGS. 28A-28B show a barrel queen used for ultrasensitive detection. FIG. 28A shows that the biomolecule presence is connected to the DNA slats (black) holding the eight loops together. Without the biomolecule, the queen falls apart and no growth can occur, even with DNA slats present in solution. FIG. 28B shows that biomolecule presence in the reaction holds the DNA slats (black) together and provides the close proximity of ssDNA scaffold for the tube structure to nucleate and grow.

FIG. 29 shows a schematic (upper panel) of a queen with six binding sites per slat and a transmission electron microscope (TEM) image (lower panel) of the queen.

FIGS. 30A-30B show a flat DNA-origami queen nucleating a staggered DNA slats ribbon. FIG. 30A depicts a flat DNA-origami queen without the bottom right hand sheet shown in FIG. 23A. FIG. 30B is a schematic explaining how the DNA slats (moving in a diagonal direction) assemble on the ssDNA scaffold on the flat queen.

FIG. 31 shows an example of a biomolecule sensing and proofreading mechanism on DNA-Origami barrel queen. Top: Biomolecule present. (1) Biomolecule binds to antibody bridge. (2) Medium gray strand is displaced via toehold-mediated strand displacement. (3) Light gray strand binds to dark gray strands, sealing bridge. Bottom: No biomolecule present. (1) No biomolecule binds to the antibody bridge. (2) Medium gray strand is displaced via toehold-mediated strand displacement, leading to no bridge being intact and the subsequent falling apart of the barrel queen (shown in FIG. 28A).

DETAILED DESCRIPTION

Nature achieves rapid and nucleation-limited growth of cytoskeletal filaments such as actin and microtubules. This is achieved by securing each additional subunit by weak interactions to 2-3 already attached subunits at the growing end of the filament. This means that if any two monomers bind to each other in solution, they will rapidly (e.g., within milliseconds) dissociate from each other, because the single interaction is so weak. It is only after four subunits come together simultaneously—a rare event—that a stable nucleus will be formed. Therefore, untriggered spontaneous nucleation will be rare. Conversely, nucleation can be triggered by providing a macromolecular “seed” that mimics a fully formed filament end.

Rapid and nucleation-limited growth are very useful features for programmable self-assembly, however technological modification of natural filaments such as actin or microtubules has many current drawbacks: (1) there is a limited understanding of how to tune the interaction strength between subunits; (2) the level of cooperativity is relatively low (the weak interactions upon binding are spread only over 2-3 subunits), therefore the suppression of spontaneous nucleation is not as robust as it could be; and (3) growth is limited to one-dimension (filament formation).

Rapid, reversible, zero-background, triggered nucleation and growth, as provided herein, can have useful applications in nanotechnology and biotechnology, such as ultrasensitive detection, and templates for miniaturized materials.

Crisscross Cooperative Assembly

The crisscross cooperative assembly technology as provided herein is based on a concept that may apply to many self-assembling molecules, including nucleic acids and proteins. For simplicity and ease of understanding, however, reference herein primarily will address crisscross cooperative assembly in the context of nucleic acids, such as deoxyribonucleic acid (DNA). A crisscross cooperative assembly system uses three basic components: a nucleating nanostructure, an initial (first) subset of nanostructures programmed to bind to the nucleating nanostructure, and another (second) subset of nanostructures programmed to bind to the nanostructures of the initial. An example of a crisscross cooperative assembly is provided in FIGS. 1A-1B, wherein the nucleating structure is referred to as a ‘queen,’ nanostructures of the first subset are referred to as ‘drones,’ and nanostructures of the second (and any subsequent) subset are referred to as ‘workers.’ The final structure, in this example, includes layers of aligned molecular rods, where each layer is rotated by some amount (e.g., 90 degrees) relative to the layer below and above. For example, one layer may be perpendicular to another adjacent (directly above or below) layer. In some embodiments, one layer is rotated 20, 30, 40, 50, 60, 70, 80 or 90 degrees relative to an adjacent layer (measured alone the length of a drone and/or worker nanorod, for example). Each intersection between rods on adjacent layers adds a small binding energy; any given rod intersects with a large number of rods below and above, and the net binding energy can be tuned (e.g., by adjusting the design of the binding interface, for example, the number of base pairs, or by adjusting subunit concentration, temperature, or salt concentration) to be large enough to achieve stable (irreversible) or slightly favorable (reversible) attachment as desired. Before assembly initiates, any spontaneous crossing between two rods in solution is short-lived, as the net energy is very low because there is only one interaction. Thus, a rod can be stably (or else slightly favorably (reversibly)) added to a pre-existing crisscross structure (many attachment points can immediately be realized), but a structure will not spontaneously assemble in the absence of a pre-existing one. There should be no growth unless a structural mimic of a pre-existing crisscross structure—a seed—is added to the solution.

An example protocol for a crisscross cooperative assembly system is as follows: (1) Design constitutive building blocks (queen, drones and workers) using DNA CAD tools. See, e.g., Douglas S M, Marblestone A H, Teerapittayanon S, Vazquez A, Church G M, Shih W M. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001-5006, 2009, incorporated herein by reference in its entirety.

Cooperative binding site sequences and number of sites on queens are tailored to modulate the activation energy of nucleation as required. (2) Construct and purify constitutive building blocks using techniques in DNA synthesis and DNA origami. (3) Mix drones and workers in solution, and add queens to initiate growth of higher order DNA structures.

Nanostructures bind to each other through cooperative binding sites. A “cooperative binding site” is the location at which two nanostructures interact (hybridize/bind). For example, a nucleating nanostructure may be programmed with multiple nucleotide base sequences, each of which is complementary to a nucleotide base sequence of one of the nanostructures of the initial subset of nanostructures. A cooperative binding site may include plug and socket sites that include plug and socket strands. A plug strand is a nucleic acid strand (single-stranded nucleic acid) attached to a nucleic acid nanostructure, such as a nanorod. A plug strand contains a nucleotide sequence that is complementary to (and this binds to) a nucleotide sequence within a cognate socket strand. Thus, a pair of plug and socket strands include nucleotide sequences that are complementary to each other such that the plug and socket strand bind (hybridize) to each other to anchor, for example, a drone to a queen or a worker to a drone (see, e.g., FIG. 17B). In some embodiments, a queen includes multiple plug strands that direct and anchor a drone that includes multiple complementary (cognate) socket strands. Likewise, a drone may include multiple plug strands that direct and anchor a worker that includes multiple complementary socket strands.

Cooperative binding sites, e.g., plug and socket strands, may also be used to assemble nucleic acid (e.g., DNA) slats onto another nucleic acid scaffold structure in a similar manner. For example, as shown in FIG. 22, DNA slats may be appended to a nucleic acid scaffold (queen) to secure the two- or three-dimensional shape of the scaffold structure. In the example, shown in FIG. 22, DNA slats are used to secure (hold together) the barrel shape of a larger scaffold nanostructure. “Growth” of these slats along the scaffold through cooperative binding sites results in a barrel-like shape that may be visualized by microscopy, for example.

Cooperative binding sites (e.g., plug and socket sequences) are arranged on a nucleating nanostructure in a spatial configuration that facilitates binding and alignment of the initial e.g., scaffold) nanostructures. The length of a cooperative binding site may vary, depending in part on the desired strength (strong v. weak) of the intended interaction between two molecules having complementary sites. In some embodiments, a cooperative binding site has a length of 5-50 nucleotides. For example, a cooperative binding site may have a length of 5-40, 5-30, 5-20, 5-10, 5-15, 10-50, 10-40, 10-30, 10-20, 30-50, 30-40, or 40-50 nucleotides. In some embodiments, a cooperative binding site has a length of 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 nucleotides. A single plug strand and/or socket strand may have a length of 5-20 (e.g., 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20) nucleotides, for example.

The number of cooperative binding sites on a nanostructure may also vary. In some embodiments, the number of cooperative binding sites on a nanostructure is 3-1000. For example, the number of cooperative binding sites on a nanostructure may be 3-900, 3-800, 3-700, 3-600, 3-500, 3-400, 3-300, 3-200, or 3-100. In some embodiments, the number of cooperative binding sites on a nanostructure is 3-10, 3-15, 3-20, 3-25, 3-30, 3-35, 3-40, 3-45 or 3-50. In some embodiments, the number of cooperative binding sites on a nanostructure is 3-15, 3-20, 3-25, 3-30, 3-35, 3-40, 3-45 or 3-50. In some embodiments, the number of cooperative binding sites on a nanostructure is 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100.

The distance between cooperative binding sites may also vary. In some embodiments, the distance between two cooperative binding sites on the same nanostructure is 20-1000 angstroms. For example, the distance between two cooperative binding sites on a nanostructures may be 20-900, 20-800, 20-700, 20-600, 20-500, 20-400, 20-300, 20-200, 20-100, 50-1000, 50-900, 50-800, 50-700, 50-600, 50-500, 50-400, 50-300, 50-200, or 50-100 angstroms. In some embodiments, the distance between two cooperative binding sites on a nanostructures is 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 125, 150, 175, 200, 250, 300, 350, 400, 450 or 500 angstroms.

In some embodiments, the distance between cooperative binding sites, for example, the distance between plug strands (and/or between socket strands) may be 5 to 100 nucleotides (or nucleotide base pairs (bp)). In some embodiments, the distance between plug strands (and/or between socket strands) is 5-20, 5-25, 5-50 or 5-100 nucleotides. In some embodiments, the distance between plug strands (and/or between socket strands) is 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100 nucleotides. In some embodiments, the distance between plug strands (and/or between socket strands) is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or 21 nucleotides. In some embodiments, the distance between plug strands (and/or between socket strands) is 42+/−21 nucleotides. For example, the distance between plug strands (and/or between socket strands) may be 21, 42 or 63 nucleotides. In some embodiments, the distance between plug strands (and/or between socket strands) is 42 nucleotides.

One nucleotide unit measures 0.33 nm. Thus, in some embodiments, the distance between cooperative binding sites, for example, the distance between plug strands (and/or between socket strands) may be 5 to 35 nanometers (nm). In some embodiments, the distance between plug strands (and/or between socket strands) is 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 nm. In some embodiments, the distance between plug strands (and/or between socket strands) is 14+/−7 nm. For example, the distance between plug strands (and/or between socket strands) may be 7, 14 or 21 nm. In some embodiments, the distance between plug strands (and/or between socket strands) is 14 nucleotides.

In some embodiments, the distance between two cooperative binding sites on a nanostructure is evenly spaced, while in other embodiments, the distances may vary. For example, the distance between a first cooperative binding site and a second cooperative binding site may be 30 angstroms, while the distance between the second cooperative binding site and a third may be 30 angstroms, 40 angstroms or 50 angstroms.

Two or more nanostructures are considered “aligned” if they are oriented in the same direction relative to one another. For example, the 5′ ends (or 3′ ends) of the nanostructures may be facing the same direction along its y axis. The top layer of the structure shown in FIG. 3B shows aligned nanorods bound to a nucleating nanostructure. The nanorods, in this example, are perpendicular to the nucleating nanostructure.

A nucleating nanostructure is required to initiate assembly of the first (initial) and second (and, thus, subsequent, e.g., third, fourth, fifth, etc.) subsets of nanostructures, and binding of the nanostructures in the first subset to the nucleating structure is required to initiate assembly of the nanostructures of the second subset. A “nucleating nanostructure” is any nanostructure programmed with binding sites that interacts strongly (irreversibly) with binding sites on each member of drone nanostructures of the initial subset, and aligns them for recruitment of subsequent subsets of worker nanostructures. That is, the binding sites between a nucleating nanostructure and nanostructures of the initial subset should be strong enough that the initial nanostructures bind to and align along the nucleating nanostructures and do not dissociate from the nucleating nanostructure under reaction conditions (e.g., isothermal, physiological conditions). A nucleating nanostructure may have a two-dimensional or a three-dimensional shape, for example.

Additional subsets of nanostructures may be added to the crisscross cooperative assembly system to propagate growth of the end structure (e.g., nanostructure, microstructure or macrostructure). For example, third, fourth and fifth subsets of nanostructures may be added. Binding of the nanostructures of the second subset to the first subset is required to initiate assembly of the nanostructures of the third subset; binding of the nanostructures of the third subset to the second subset is required to initiate assembly of the nanostructures of the fourth subset; and so on. The user-defined end structure may be assembled in one dimension, two dimensions (see, e.g., FIG. 3B) or three-dimensions.

Each subset of nanostructures (e.g., nanorods) should follow a specific set of binding energy parameters. More specifically, the initial subset of nanostructures (e.g., nanorods) should bind strongly (irreversibly) to and form an aligned layer (where each nanostructure is oriented in the same direction relative to one another) along the nucleating nanostructure. The nanostructures (e.g., nanorods) of the initial subset should not interact with (bind to) each other. Likewise, nanostructures (e.g., nanorods) of a subsequent subset should not interact with (bind to) each other. Further, in the absence of a nucleating structure, any nanostructure (e.g., nanorod) from the initial subset should have only one weak (reversible) interaction with any other nanostructure (e.g., nanorod) from a subsequent subset. In the presence of a nucleating structure, a single nanostructure (e.g., nanorod) from an initial subset may interact with more than one nanostructure (e.g., nanorod) from a subsequent subset, and a single nanostructure (e.g., nanorod) from a subsequent subset may interact with more than one nanostructure (e.g., nanorod) from the initial subset. For example, with reference to FIG. 1B, a single nanostructure (e.g., nanorod) may bind to eight other nanostructure (e.g., nanorod), although the single nanostructure (e.g., nanorod) binds to each of the eight nanostructure (e.g., nanorod) only once to form two layers having a ‘crisscross’ pattern.

A “strong interaction” refers to binding that is engaged more than 50% (e.g., more than 60%, 70%, 80% or 90%) of the time that the binding nanostructures are in a reaction together (the dissociation constant is lower than the concentration of the species/nanostructures in excess).

A “weak interaction”—refers to binding that is engaged less than 1% of the time that the binding nanostructures are in a reaction together (the dissociation constant is at least 100 times higher than the concentration of the species/nanostructures in excess).

A nucleating nanostructure may bind to two or more other nanostructures. In some embodiments, a nucleating nanostructure binds to 5-1000 nanostructures (e.g., DNA nanorods). For example, a nucleating nanostructure may bind to 3-900, 3-800, 3-700, 3-600, 3-500, 3-400, 3-300, 3-200, or 3-100 nanostructures. In some embodiments, a nucleating nanostructure binds to 3-10, 3-15, 3-20, 3-25, 3-30, 3-35, 3-40, 3-45 or 3-50 nanostructures. In some embodiments, a nucleating nanostructure binds to 10-15, 10-20, 10-25, 10-30, 10-35, 10-40, 10-45 or 10-50 nanostructures. In some embodiments, a nucleating nanostructure binds to 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100 nanostructures (e.g., DNA nanorods).

Thus, a single subset of nanostructures (nanostructures programmed to interact with a single nucleating nanostructure) may comprise 3-900, 3-800, 3-700, 3-600, 3-500, 3-400, 3-300, 3-200, or 3-100 nanostructures. In some embodiments, a single subset of nanostructures comprises 3-10, 3-15, 3-20, 3-25, 3-30, 3-35, 3-40, 3-45 or 3-50 nanostructures. In some embodiments, a single subset of nanostructures comprises 10-15, 10-20, 10-25, 10-30, 10-35, 10-40, 10-45 or 10-50 nanostructures. In some embodiments, a single subset of nanostructures comprises 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100 nanostructures (e.g., DNA nanorods).

A “subset of nanostructures” refers to a specific group of nanostructures that are similar in size (have similar dimensions) and structure/shape and are programmed to bind to either the nucleating nanostructure (the initial subset) or to a pre-existing layer formed by alignment and binding of other nanostructures that have already aligned and bound to the nucleating structure or nanostructures of another pre-existing layer.

Nanostructures within a defined subset are programmed not bind to each other. Thus, in some embodiments, less than 10% of the nanostructures of a subset bind to another nanostructure of the same subset. In some embodiments, less than 9%, less than 8%, less than 7%, less than 6%, less than 5%, less than 4%, less than 3%, less than 2%, less than 1%, less than 0.5%, less than 0.2%, or less than 0.1% of the nanostructures of a subset bind to another nanostructure of the same subset. In some embodiments, none of the nanostructures of a subset bind to another nanostructure of the same subset.

With crisscross cooperative assembly, nanostructures are aligned to form multiple layers, each layer rotated by some degree relative to adjacent layers (above and below). An example of two layers rotated relative to one another is shown in FIG. 1B. The top layer of aligned nanorods is rotated 90 degrees relative to the bottom layer of aligned nanorods. The degree of rotation between two adjacent layers may vary. In some embodiments, one layer is rotated 10-90 degrees, 20-90 degrees, 30-90 degrees, 40-90 degrees, 50-90 degrees, 60-90 degrees, 70-90 degrees, or 80-90 degrees relative to an adjacent layer. In some embodiments, one layer is rotated 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, or 90 degrees relative to an adjacent layer.

Nucleic Acid Nanostructures

A “nucleic acid nanostructure,” including a “DNA nanostructure,” refers to a nanostructure (e.g., a structure that is between 0.1 nm and 1 μm (e.g., 0.1 nm and 100 nm) in each spatial dimension, e.g., 1D, 2D or 3D) that is rationally designed to self-assemble (is programmed) into a pre-determined, defined shape that would not otherwise assemble in nature. The use of nucleic acids to build nanostructures is enabled by strict nucleotide base pairing rules (e.g., A binds to T, G binds to C, A does not bind to G or C, T does not bind to G or C), which result in portions of strands with complementary base sequences binding together to form strong, rigid structures. This allows for the rational design of nucleotide base sequences that will selectively assemble (self-assemble) to form nanostructures.

Examples of nucleic acid (e.g., DNA) nanostructures include, but are not limited to, DNA origami structures, in which a long scaffold strand (e.g., at least 500 nucleotides in length) is folded by hundreds (e.g., 100, 200, 200, 400, 500 or more) of short (e.g., less than 200, less than 100 nucleotides in length) auxiliary strands into a complex shape (Rothemund, P. W. K. Nature 440, 297-302 (2006); Douglas, S. M. et al. Nature 459, 414-418 (2009); Andersen, E. S. et al. Nature 459, 73-76 (2009); Dietz, H. et al. Science 325, 725-730 (2009); Han, D. et al. Science 332, 342-346 (2011); Liu, W et al. Angew. Chem. Int. Ed. 50, 264-267 (2011); Zhao, Z. et al. Nano Lett. 11, 2997-3002 (2011); Woo, S. & Rothemund, P. Nat. Chem. 3, 620-627 (2011); Tørring, T. et al. Chem. Soc. Rev. 40, 5636-5646 (2011)). Other more modular strategies have also been used to assemble DNA tiles (Fu, T. J. & Seeman, N. C. Biochemistry 32, 3211-3220 (1993); Winfree, E. et al. Nature 394, 539-544 (1998); Yan, H. et al. Science 301, 1882-1884 (2003); Rothemund, P. W. K. et al. PLoS Biol. 2, e424 (2004); Park, S. H. et al. Angew. Chem. Int. Ed. 45, 735-739 (2006); Schulman, R. & Winfree, E. Proc. Natl Acad. Sci. USA 104, 15236-15241 (2007); He, Y. et al. Nature 452, 198-201 (2008); Yin, P. et al. Science 321, 824-826 (2008); Sharma, J. et al. Science 323, 112-116 (2009); Zheng, J. P. et al. Nature 461, 74-77 (2009); Lin, C. et al. ChemPhysChem 7, 1641-1647 (2006)) or RNA tiles (Chworos, A. et al. Science 306, 2068-2072 (2004); Delebecque, C. J. et al. Science 333, 470-474 (2011)) into periodic (Winfree, E. et al., Nature 394, 539-544 (1998); Yan, H. et al. Science 301, 1882-1884 (2003); Chworos, A. et al. Science 306, 2068-2072 (2004); Delebecque, C. J. et al. Science 333, 470-474 (2011)) and algorithmic (Rothemund, P. W. K. et al. PLoS Biol. 2, e424 (2004)) two-dimensional lattices (Seeman, N. C. J. Theor. Biol. 99, 237-247 (1982); Park, S. H. et al. Angew. Chem. Int. Ed. 45, 735-739 (2006)), extended ribbons (Schulman, R. & Winfree, E. Proc. Natl Acad. Sci. USA 104, 15236-15241 (2007); Yin, P. et al. Science 321, 824-826 (2008)) and tubes (Yan, H. et al. Science 301, 1882-1884 (2003); Yin, P. et al. Science 321, 824-826 (2008); Sharma, J. et al. Science 323, 112-116 (2009)), three-dimensional crystals (Zheng, J. P. et al. Nature 461, 74-77 (2009)), polyhedral (He, Y. et al. Nature 452, 198-201 (2008)) and simple finite two-dimensional shapes (Chworos, A. et al. Science 306, 2068-2072 (2004); Park, S. H. et al. Angew. Chem. Int. Ed. 45, 735-739 (2006)).

Thus, crisscross cooperative assembly building blocks (e.g., nucleating nanostructures and subsets of nanostructures) may be one of a number of nucleic acid nanostructure shapes, including, but not limited to, rods/tubes, sheets, ribbons, lattices, cubes, spheres, polyhedral, or another two-dimensional or three-dimensional shape. In some embodiments, a nanostructure has junction(s), branch(es), crossovers, and/or double-crossovers formed by nucleotide base pairing of two or more nucleic acid strands (see, e.g., Mao, C. PLoS Biology, 2(12), 2036-2038, 2004).

In some embodiments, a nucleic acid nanostructure has a handle and barrel shape, similar to that depicted in FIG. 22.

The versatile and stable nature of DNA origami enables the construction of various individual architectures that can be designed in a particular way, to facilitate to cooperative assembly of larger structures. In one example, each component is a separately folded DNA-origami structure. FIG. 3A shows an example of a DNA origami queen, drone and worker, whereby the drone and worker are of identical architecture (six helix bundle DNA nanotubes). Queen, drones and workers can then assemble in a cooperative manner to form higher order 1D, 2D and 3D structures (FIG. 3B). 3D structures are contemplated by merging 1D and 2D design principles. For example, FIG. 22 depicts a 3D queen nanostructure assembling with 2D drone/worker slats to form a barrel shape.

A nucleic acid (e.g., DNA) slat is a slat-shaped nanostructure that is composed of DNA. A slat may be an antiparallel-crossover single-stranded slat (AXSSS) comprising single strands that cross a partnering single strand only once. Also provided herein are paranemic crossover slats that include a pair of strands that cross another pair of strands.

Similar to the larger scale DNA-origami crisscross cooperative assembly, single-stranded DNA can be used to achieve cooperative assembly of higher order structures. In order to achieve this, drones and workers are replaced with oligonucleotides of various lengths (depending on the proposed architecture) that can assemble onto a DNA-origami queen nucleation site (shown in FIG. 4) or onto a single stranded DNA catenane structure shown in FIG. 5, FIG. 22 and FIGS. 24A-24B. The ring structures depicted in FIG. 5 are comprised of single-stranded DNA that has exposed binding sites for drone and worker oligonucleotides. In another example, the components are folded into a DNA origami barrel queen (FIGS. 24A-24B). The scaffold can be tiled with extended DNA slats (slats) capable of seeding further DNA slats, leading to growth of the structure. Generally, the DNA slats work in two steps: first, folding the origami queen site (for example, mixing M13 scaffold and staple strands), and second, mixing the crude DNA origami queen reaction with DNA slats, leading to growth of the structure. Varying salt concentrations, temperatures, and DNA slat concentration can alter the binding energy of the various sub-components, leading to reversible or irreversible binding, for example.

Typically, nucleic acid nanostructures do not contain coding sequences (sequences that code for a full length mRNA or protein), thus, nucleic acid nanostructures do not contain a promoter or other genetic elements that control gene/protein expression. An individual single-stranded nucleic acid (e.g., DNA strand or RNA strand without secondary structure), or an individual double-stranded nucleic acid (e.g., without secondary structure), for example, double helices found in nature or produced synthetically or recombinantly (e.g., such as a plasmid or other expression vector), are specifically excluded from the definition of a nucleic acid nanostructure.

Nanostructures, in some embodiments, have a void volume, which is the combine volume of space between nucleic acids that form a nanostructures. It should be understood that “space” includes fluid-filled space. Thus, a nanostructure in solution, have a void volume of 25% may include 75% nucleic acids and 25% reaction buffer (filling the 25% void volume of the nanostructure). In some embodiments, a nanostructure in solution, e.g., in reaction buffer, may have a void volume of at least 10% (e.g., 10-90%, 10-80%, 10-70%, 10-60%, 10-50%, 10-40%, or 10-30%), at least 20% (e.g., 20-90%, 20-80%, 20-70%, 20-60%, 20-50%, 20-40%, or 20-30%), at least 30%, (e.g., 30-90%, 30-80%, 30-70%, 30-60%, 30-50%, or 30-40%), at least 40% (e.g., 40-90%, 40-80%, 40-70%, 40-60%, or 40-50%), at least 50% (e.g., 50-90%, 50-80%, 50-70%, or 50-60%), at least 60% (e.g., 60-90%, 60-80%, or 60-70%), at least 70% (e.g., 70-90% or 70-80%), or at least 80% (e.g., 80-90%). In some embodiments, a nanostructure has a void volume of 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90%.

A “nucleic acid nanorod,” including a “DNA nanorod” is a nucleic acid (e.g., DNA) nanostructure in the shape of a rod. A nanorod is a three-dimensional cylindrical shape having a length longer than its diameter. Examples of nanorods are depicted in FIGS. 1A-1B and FIGS. 3A-3B. In some embodiments, a nucleic acid nanorod comprises six helix bundles. For example, six DNA double helices may be connected to each other at two crossover sites. DNA double helices with 10.5 nucleotide pairs per turn facilitate the programming of DNA double crossover molecules to form hexagonally symmetric arrangements when the crossover points are separated by seven or fourteen nucleotide pairs (see, e.g., Mathieu F. et al. Nano Lett. 5(4), 661-664 (2005)). Other methods of assembling nucleic acid nanorods (also referred to as nanotubes) may be used (see, e.g., Feldkamp, U. et al. Angew. Chem. Int. Ed. 45(12), 1856-1876 (2006); Hariri A. et al. Nature Chemistry, 7, 295-300 (2015)).

The length and diameter of a nanorod (or other nanostructure) may vary. In some embodiments, a nanorod (or other nanostructure) has a length of 10-100 nm, or 10-500 nm. For example, a nanorod may have a length of 10-500 nm, 10-400 nm, 10-300 nm, 10-200 nm, 10-100 nm, 10-90 nm, 10-80 nm, 10-70 nm, 10-60 nm, 10-50 nm, 10-30 nm, or 10-20 nm. In some embodiments, a nanorod has a length of 100-500 nm, 200-500 nm, or 300-500 nm. In some embodiments, a nanorod has a length of 100 nm, 150 nm, 200 nm, 250 nm, 300 nm, 350 nm, 400 nm, 450 nm or 500 nm. In some embodiments, a nanorod has a length of 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100 nm. In some embodiments, the length of a nanorod (or other nanostructure) is longer than 100 nm (e.g., 100-1000 nm), or shorter than 10 nm (e.g., 1-10 nm). In some embodiments, a nanorod (or other nanostructure) has a diameter of 5-90 nm. For example, a nanorod may have a diameter of 5-80 nm, 5-70 nm, 5-60 nm, 5-50 nm, 5-30 nm, 5-20 or 5-10 nm. In some embodiments, a nanorod has a diameter of 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85 or 90 nm. In some embodiments, the diameter of a nanorod is longer than 9 nm, or shorter than 5 nm. Thus, in some embodiments, a nanorod (or other nanostructure) has a circumference of 15-300 nm (C≈3.14×d).

A nucleic acid nanostructure, such as a nanorod, is considered “elongated,” if the length of the nanostructure is longer than its width/diameter (e.g., by at least 10%, 20%, 25%, 50%, 100%, or 200%).

Nucleic acid nanostructures are typically nanometer-scale structures (e.g., having lengths of 1 to 1000 nanometers). In some embodiments, however, the term “nanostructure” herein may include micrometer-scale structures (e.g., assembled from more than one nanometer-scale or micrometer-scale structure). In some embodiments, a nanostructure has a dimension (e.g., length or width/diameter) of greater than 500 nm or greater than 1000 nm. In some embodiments, a nanostructure has a dimension of 1 micrometer to 2 micrometers. In some embodiments, a nanostructure has a dimension of 10 to 500 nm, 10 to 450 nm, 10 to 400 nm, 10 to 350 nm, 10 to 300 nm, 10 to 250 nm, 10 to 200 nm, 10 to 150 nm, 10 to 100 nm, 10 to 50 nm, or 10 to 25 nm. In some embodiments, the nanostructure has a dimension of 500 to 450 nm, 500 to 400 nm, 500 to 350 nm, 500 to 300 nm, 500 to 250 nm, 500 to 200 nm, 500 to 150 nm, 500 to 100 nm, 500 to 50 nm, or 500 to 25 nm. In some embodiments, the nanostructure has a dimension of 10, 25, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, or 500 nm.

A nucleic acid nanostructure is considered to “self-assemble.” Bottom up, self-assembly refers to the process by which molecules adopt a defined arrangement without guidance or management from an outside source. Although, it should be understood that with synthetic nucleic acid self-assembly, as provided herein, the nucleotide base sequences that guide assembly of nucleic acids are artificially designed, and the corresponding nucleic acids are accordingly synthesized by an outside source, such as one of skill in the art (using, for example, standard nucleic acid synthesis techniques). That is, one of ordinary skill in the art can ‘program’ nucleotide base sequences within a single nucleic acid strand or between two difference nucleic acid strands to selectively bind to each other in solution based on a strict set of nucleotide base pairing rules (e.g., A binds to T, G binds to C, A does not bind to G or C, T does not bind to G or C). Self-assembly may be intramolecular (folding) or intermolecular.

The nanostructures and, thus, nanostructures, microstructures and macrostructures assembled from smaller nanostructures, are “rationally designed.” A nanostructure, as discussed above, does not assemble in nature. Nucleic acid strands for use in crisscross cooperative assembly are ‘programmed’ such that among a specific population of strands, complementary nucleotide base sequences within the same strand or between two different strands bind selectively to each other to form a complex, user-defined structure, such as a rod/tube, ribbon, lattice, sheet, polyhedral, cube, sphere, or other two-dimensional or three-dimensional shape. A nanostructure may have a regular shape (sides that are all equal and interior angles that are all equal) or an irregular shape (sides and angles of any length and degree).

Methods of Crisscross Cooperative Assembly

Self-assembly of a nucleating nanostructure and subsets of nanostructures occurs, in some embodiments, in a ‘one-pot’ reaction, whereby all nucleic acid nanostructures of a crisscross cooperative assembly system are combined in a reaction buffer, and then the reaction buffer is incubated under conditions that result in self-assembly of all of the nucleic acid nanostructures.

Conditions that result in self-assembly of nucleic acid nanostructures of a crisscross cooperative assembly reaction may vary depending on the size, shape, composition and number of nucleic acid nanostructures in a particular reaction. Such conditions may be determined by one of ordinary skill in the art, for example, one who rationally designs/programs the nanostructures to self-assemble.

A crisscross cooperative assembly method may be performed at a variety of temperatures. In some embodiments, a crisscross cooperative assembly method is performed at room temperature (˜25° C.) or 37° C. A crisscross cooperative assembly method may be performed at a temperature lower than 25° C. or higher than 37° C.

The salt concentration of the reaction buffer in which a crisscross cooperative assembly reaction is performed may also vary. In some embodiments, the reaction buffer comprises MgCl₂ salt at a concentration of 1 mM-10 mM (e.g., 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM or 10 mM). In some embodiments, the reaction buffer comprises NaCl at a concentration of 100 mM-500 mM (e.g., 100 mM, 200 mM, 300 mM, 400 mM or 500 mM). In some embodiments, a crisscross cooperative assembly method is performed under high-salt conditions. Thus, in some embodiments, the reaction buffer comprises MgCl₂ salt at a concentration of at least 20 mM (e.g., 20-500 mM, or 20-200 mM). In some embodiments, the reaction buffer comprises NaCl at a concentration of at least 1 M (e.g., 1-2 M, 1-3 M, 1-4 M, or 1-5 M).

In any given reaction, the number of initial nanostructures (drones) exceeds the number of nucleating nanostructures (queens). Thus, in some embodiments, the ratio of nucleating nanostructure to non-nucleating nanostructure (e.g., a drone from an initial subset, or a worker from a subsequent subset) is 1:10-1:10¹² (trillion). For example, the ratio of nucleating nanostructure to non-nucleating nanostructure may be 1:10-1:1000, 1:10-1:500, 1:10-1:100, 1:10-1:75, 1:10-1:50, or 1:10-1:25. In some embodiments, the ratio of nucleating nanostructure to non-nucleating nanostructure is 1:1000, 1:500, 1:100, 1:90, 1:80, 1:70, 1:60, 1:50, 1:40, 1:30, 1:20 or 1:10.

In some embodiments, a crisscross cooperative assembly reaction is incubated for 2-96 hours. For example, a crisscross cooperative assembly reaction may be incubated for 2-24 hours, 2-30 hours, 2-36 hours, 2-42 hours, 2-48 hours, 2-54 hours, 2-60 hours, 2-66 hours, 2-72 hours, 2-78 hours, 2-84 hours, 2-90 hours, or 2-96 hours. In some embodiments, a crisscross cooperative assembly reaction is incubated for 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, or 72 hours. In some embodiments, a crisscross cooperative assembly reaction is incubated for 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70 or 72 hours.

Biosensors

In some embodiments, the crisscross assembly products may be used as biosensors that are capable of detecting a selected biomolecule (analyte) using a variety of different mechanisms and the systems described herein. For example, in such systems, the presence of a biomolecule can be used to trigger crisscross assembly, which can then be detected (visualized), indicating the presence of the biomolecule.

The biomolecule may be detected using a ring system. As depicted in FIG. 6, the large DNA ring (“host ring”), single-stranded DNA, may be split to incorporate a biomolecule capture site (analyte test site) to bind macromolecules in biological samples. The DNA ring loops through and encloses a number of discrete, separate “guest” rings, which are single-stranded DNA and function as catenane queens, so that the guest rings are catenated on the host ring, similar to individual beads on a bracelet. In some embodiments, the guest rings are independently formed from separate single-stranded nucleic acids (see, e.g., FIGS. 5 and 6), while in other embodiments, the guest rings are formed from a long single nucleic acid strand assembled into multiple (e.g., vertically stacked) rings (see, e.g., FIGS. 27A and 27B). The number of guest rings can be 2, 3, 4, or 5 or more. In embodiments, each guest ring (catenane queen) comprises binding sites for drone and worker oligonucleotides and is therefore capable of crisscross assembly. In embodiments, the plurality of catenated guest rings when in close proximity forms a catenane queen comprising binding sites (e.g., plug strands) for drone and worker nucleic acids and/or structures and is thus capable of crisscross assembly. The number of binding sites per guest ring can vary, and may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100. A biomolecule test site, located near the biomolecule capture site may also formed.

The presence of the biomolecule, in some embodiments may be detected in mixtures, such as biological samples, as follows. First, a biological sample is mixed with a high concentration of the catenane queen, allowing macromolecules of interest bind the biomolecule capture site. Then, a chemical reaction is used to reversibly cleave the biomolecule capture site. Catenane queens not bound to the target biomolecule will fall apart more quickly compared to those held together by the target biomolecule. The remaining catenane queens in the test mixture are re-ligated at the biomolecule test site. Subsequently, drones and workers are added to the test mixture to amplify remaining intact queens using readily observable micrometer-scale DNA structures. This system is modular, and the biomolecule capture site may be customized to bind disease markers, including proteins or nucleic acid sequences.

Ultraspecific biosensors can also be created by adding a biomolecule detection system to the multiple guest-ring (e.g., guest-loop) catenane systems with DNA slats, as depicted in FIGS. 27-28. In this example, a barrel queen is used; however, other 3-dimensional shapes are also possible (e.g., sheets, blocks and dendrimers). An example of the production of a barrel queen (a rolled sheet) is described above.

An example of a DNA slat is depicted in FIGS. 21A and 21B.

Using a scaffold for DNA origami, for example an M13 scaffold and staple strands, a multiple guest-ring catenane system can be formed. For example, in FIG. 27, an eight-loop system is formed in a one-pot reaction. The number of loops (rings) can be varied, depending on the design of the system, and may be 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 loops. Additional loops may be used. Unlike the system described above, the handles (elongated structures) and loops (rings) are all part of the same single-stranded DNA (e.g., M13 DNA); the handle structures are programmed to link together by specific staple strands (slats). The system is designed around the specific staple strands/slats; in the presence of biomolecule, they hold the structure together and growth can occur from the parallel loops when drones and workers are added (FIG. 28B). In the absence of biomolecule, the staple strands/slats release the structure, and no growth can occur as the queen falls apart and the binding sites are not close enough for nucleation and growth even in the presence of drones and workers (FIG. 28A). The presence of the structures can be detected using any one of the methods described above, or with any method known in the art.

DNA slats or other nucleic acids of a biosensor may be modified with one or more switchable bridges. A “switchable bridge” is a link between functional groups that forms or breaks in the presence of a particular agent (e.g., reaction agent or dissociation agent). Examples of switchable bridges include bonds formed via a “click chemistry” reaction (e.g., a between an azide and an alkyne), protein-protein binding (e.g., one or more antibodies binding to a target protein/antigen), a disulfide bond (between two thiols).

Thus, some aspects of the present disclosure provide a biosensor comprising (i) a first DNA slat comprising a first functional group (e.g., an azide or alkyne), a first binding partner (e.g., an antibody, aptamer or nanobody), and a second functional group (e.g., a thiol or nucleic acid), and (ii) a second DNA slat comprising a third functional group (e.g., a thiol or nucleic acid), a second binding partner (e.g., an antibody, aptamer or nanobody), and a fourth functional group (e.g., an azide or alkyne), wherein the first and fourth functional groups react in the presence of a reaction agent to form a link (e.g., a covalent link), wherein the first and fourth binding partners bind specifically to a biomolecule of interest to form a link (e.g., non-covalent link), and wherein the second and third functional groups form a link (e.g., a covalent link) that breaks in the presence of a dissociation agent.

In some embodiments, a biosensor comprises a first DNA slat comprising an azide, an antibody, and a thiol group, and a second DNA slat comprising an alkyne, an antibody, and a thiol group, wherein antibody of (i) and the antibody of (ii) bind specifically to a biomolecule of interest.

A “first biomolecule binding partner” and a “second biomolecule binding partner” are any molecules that bind to the same target biomolecule to form a switchable bridge linking DNA slats to each other (via a non-covalent link). In some embodiments, the first and second biomolecule binding partners are proteins or peptides. For example, the first and second biomolecule binding partners may be antibodies that bind to different epitopes of the same antigen. Thus, in some embodiments, the first and second biomolecule binding partners are antibodies (e.g., monoclonal, polyclonal, human, humanized or chimeric). In some embodiments, the first and second biomolecule binding partners are antibody fragments (e.g., Fab, F(ab′)2, Fc, scFv, or vhh). The biomolecule binding partners may also be nanobodies or aptamers. Other protein-protein binding partners may be used.

A “first functional group” and a “fourth functional group” are functional groups that react with each other to form a link (bond, such as a covalent bond or a non-covalent bond), which forms a switchable bridge linking the DNA slats to each other. In some embodiments, this bridge is formed through a click chemistry (azide-alkyne cycloaddition) reaction (e.g., V. V. Rostovtsev, et al., Angew. Chem. Int. Ed., 2002, 41, 2596-2599; and F. Himo, et al. J. Am. Chem. Soc., 2005, 127, 210-216, each of which is incorporated herein by reference). Thus, in some embodiments, one of the first or fourth functional group is an azide, while the other of the first or fourth functional groups is an alkyne. For example, the first functional group may be azide, and the fourth functional group may be trans-cyclooctene (TCO). Other click chemistry functional groups may be used.

A “second functional group” and a “third functional group” are functional groups that react with each other to form a link (bond, such as a covalent bond or a non-covalent bond), which forms yet another switchable bridge linking the DNA slats to each other. This bridge breaks (dissociates) in the presence of a dissociation agent. A “dissociation agent” is an agent (e.g., chemical) that breaks the bond (e.g., covalent bond) between the second and third functional groups. In some embodiments, the second and third functional groups are thiol groups that react with each other to form a disulfide bridge. Thus, in some embodiments, the dissociation agent is dithiothreitol (DTT). In some embodiments, the concentration of DTT is 50 mM-200 mM. For example, the concentration of DTT may be 100 mM. Other functional groups may be used.

Additional Embodiments

-   1. A composition, comprising:     -   (a) nucleating nanostructures;     -   (b) a first subset of elongated nanostructures, wherein less         than 10% of the nanostructures of (b) bind to each other, and         wherein the nanostructures of (b) irreversibly bind to a         nucleating nanostructure of (a); and     -   (c) a second subset of elongated nanostructures, wherein less         than 10% of the nanostructures of (c) bind to each other,         wherein, in the absence of a nucleating nanostructure, a         nanostructure of (b) can reversibly binding to a nanostructure         of (a) only at a single position on the nanostructure of (a),         and wherein, in the absence of a nucleating nanostructure, a         nanostructure of (a) can reversibly binding to a nanostructure         of (b) only at a single position on the nanostructure of (b). -   2. The composition of embodiment 1 further comprising (d) a third     subset of elongated nanostructures, wherein less than 10% of the     nanostructures of (d) bind to each other. -   3. The composition of embodiment 1 or 2, wherein the nanostructures     of (b) are aligned in one direction and irreversibly bound to a     nucleating nanostructure of (a) to form a first layer. -   4. The composition of embodiment 3, wherein the nanostructures     of (c) are aligned in one direction and bound to nanostructures of     the first layer to form a second layer, wherein first layer is     rotated by 10 degrees to 170 degrees relative to the second layer. -   5. The composition of embodiment 4, wherein the first layer is     rotated by 90 degrees relative to the second layer. -   6. The composition of embodiment 4 or 5, wherein the nanostructures     of (d) are aligned in one direction and bound to nanostructures of     the second layer to form a third layer, wherein second layer is     rotated by 10 degrees to 170 degrees relative to the third layer. -   7. The composition of embodiment 6, wherein the first layer is     rotated by 90 degrees relative to the second layer. -   8. The composition of any one of embodiments 1-7, wherein the     nucleating nanostructures of (a), the nanostructures of (b), the     nanostructures of (c), and/or the nanostructures of (d) are nucleic     acid nanostructures. -   9. The composition of embodiment 8, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) are DNA nanostructures. -   10. The composition of embodiment 8 or 9, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) comprise a long nucleic     acid strand bound to multiple nucleic acid strands that are shorter     than the long nucleic acid strand. -   11. The composition of embodiment 8 or 9, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) comprise multiple nucleic     acid strands, each having a length of less than 200 nm. -   12. The composition of any one of embodiments 1-6, wherein the     nucleating nanostructures of (a), the nanostructures of (b), the     nanostructures of (c), and/or the nanostructures of (d) are protein     nanostructures. -   13. The composition of any one of embodiments 1-12, wherein the     nucleating nanostructures of (a), the nanostructures of (b), the     nanostructures of (c), and/or the nanostructures of (d) are     rod-shaped. -   14. A method, comprising: -   combining in reaction buffer -   (a) nucleating nanostructures, -   (b) a first subset of elongated nanostructures, wherein less than     10% of the nanostructures of (b) bind to each other, and wherein the     nanostructures of (b) irreversibly bind to a nucleating     nanostructure of (a), and -   (c) a second subset of elongated nanostructures, wherein less than     10% of the nanostructures of (c) bind to each other, -   wherein, in the absence of a nucleating nanostructure, a     nanostructure of (b) can reversibly binding to a nanostructure     of (a) only at a single position on the nanostructure of (a), and     wherein, in the absence of a nucleating nanostructure, a     nanostructure of (a) can reversibly binding to a nanostructure     of (b) only at a single position on the nanostructure of (b); and     -   incubating the reaction buffer comprising (a), (b) and (c) under         conditions that result in binding of the nanostructures of (b)         to the nucleating nanostructures of (a) and result in binding of         the nanostructures of (c) to the nanostructures of (b) to form a         hierarchical structure. -   15. The method of embodiment 14, wherein the reaction buffer further     comprises (d) a third subset of elongated nanostructures, wherein     less than 10% of the nanostructures of (d) bind to each other. -   16. The method of embodiment 14 or 15, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) are nucleic acid     nanostructures. -   17. The method of embodiment 16, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) are DNA nanostructures. -   18. The method of embodiment 16 or 17, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) comprise a long nucleic     acid strand bound to multiple nucleic acid strands that are shorter     than the long nucleic acid strand. -   19. The method of embodiment 16 or 17, wherein the nucleating     nanostructures of (a), the nanostructures of (b), the nanostructures     of (c), and/or the nanostructures of (d) comprise multiple nucleic     acid strands, each having a length of less than 200 nm. -   20. The method of any one of embodiments 14-16, wherein the     nucleating nanostructures of (a), the nanostructures of (b), the     nanostructures of (c), and/or the nanostructures of (d) are protein     nanostructures. -   21. The method of any one of embodiments 14-20, wherein the     nucleating nanostructures of (a), the nanostructures of (b), the     nanostructures of (c), and/or the nanostructures of (d) are     rod-shaped. -   22. A composition, comprising:     -   (a) nucleating DNA nanostructures;     -   (b) a first subset of elongated DNA nanorods, wherein less than         10% of the nanostructures of (b) bind to each other, and wherein         the DNA nanorods of (b) irreversibly bind to a nucleating DNA         nanostructure of (a); and     -   (c) a second subset of elongated DNA nanorods, wherein less than         10% of the DNA nanorods of (c) bind to each other, wherein, in         the absence of a nucleating DNA nanostructure, a DNA nanorod         of (b) can reversibly binding to a DNA nanorod of (a) only at a         single position on the DNA nanorod of (a), and wherein, in the         absence of a nucleating DNA nanostructure, a DNA nanorod of (a)         can reversibly binding to a DNA nanorod of (b) only at a single         position on the DNA nanorod of (b).

EXAMPLES Example 1

The examples demonstrates assembly of a nucleating nanostructures (queen). The sharpest bands from a screen of nucleic acid self-assembly reactions were selected and subjected to a 2 minute incubation at 90° C. for denaturing and then an 18 hour ramp. The gel (FIG. 9A) was 2% agarose (10 μL ethidium bromide, c=10 mg/mL), and run at 60V for 240 minutes in 0.5×TBE and 11 mM MgCl₂. A seesaw experiment was performed, whereby the temperature was varied between 65-60° C. (A), 60-55° C. (B), 65-55° C. (C), and 60-50° C. (D). The structures were purified using band excision of the gel, followed by 15 minutes at 16 k×g FreezeNSqueeze and then stained with 2% UF following a 2 minute ddH₂O post-wash. The queen folded well, and no noticeable difference was observed between conditions A through D on the seesaw experiment (FIG. 9B).

The experiment was repeated with different nanostructures that function as ‘drones’. The sharpest bands from a large screen were selected and subjected to 2 minute incubation at 90° C. for denaturing and then an 18 hour ramp. The gel (FIG. 10A) was 2% agarose (10 μL ethidium bromide, c=10 mg/mL), and run at 60V for 240 minutes in 0.5×TBE and 11 mM MgCl₂. In this experiment, the temperature was varied between 70-60° C. (A), 65-55° C. (B), 65-60° C. (C), and 60-55° C. (D). The structures were purified using band excision of the gel, followed by 15 minutes at 16 k×g FreezeNSqueeze and then stained with 1% UF. The drone folded well, and there was no noticeable difference between conditions A through D on the seesaw experiment (FIG. 10B).

Next, the assembly of a queen together with a drone was examined (FIG. 11). The following conditions were tested (with a 1:1:1 ratio): queen—all sites closed and both drones; queen—site 0 exposed and both drones; queen—site 1 exposed and both drones; queen—site 0/1 exposed and both drones; and queen—all sites exposed and both drones. Assembly was achieved through a 72 hour incubation period at 25° C., and the structures were purified by band excision of the gel, followed by 15 minutes at 16 k×g FreezeNSqueeze followed by 2% UF staining. The samples were run on a 2% agarose gel (10 μl ethidium bromide, c=10 mg/mL), and run at 60V for 240 minutes in 0.5×TBE and 11 mM MgCl₂. Approximately 10-15 ng of each structure were observed.

Example 2

A similar system was built using single-stranded DNA instead of 6 helix bundles. A schematic of the nucleating nanostructure architecture is shown in FIG. 12A. The single strands each contain binding and linker regions, including a 5 bp binding region and 3 and 5 nucleotide linker (poly T) regions. An exemplary 5 bp, 2 nucleotide linker is shown below:

62 mer (SEQ ID NO: 683) TGCAA TTT AATTC TTT TAGCA TTT CAATA TTT GTAGA TTT GAGAA TTT CGTTTT TTT ATTCA.

The workers stack on top of drones in layers (FIGS. 12B-12G). The queen was shown to fold in both 5C or 10C steps (FIG. 12A). Folding occurred after a 2 minute 90° C. denaturing period and then an 18 hour ramp. The samples were run on a 2% agarose gel (10 μl ethidium bromide, c=10 mg/mL), and run at 60V for 240 minutes in 0.5×TBE and 11 mM MgCl₂. The queen was incubated under a 50-40° C. thermal ramp, with 6 mM MgCl₂. The resulting structure is shown in FIG. 13B.

Example 3

Assembly without the queen and only the workers (both short and long linkers) was examined. In this example, workers did not assemble under conditions with high salt concentration (1M NaCl, up to 15 mM MgCl₂), low temperature (4° C.), and a high concentration of workers (3.125 μM). Other conditions, including 10-20 mM PEG and high salt and a high concentration of oligonucleotides were also tested. No assembly occurred. FIGS. 14A-14B show drone/workers at different concentrations successfully assembling with queens. The structures were purified with band excision, 15 minutes at 16 k×g FreezeNSqueeze and 2% UF staining. Without the queens, there was no sign of assembly (FIG. 14C).

The duplex length was then increased to 8 bp and linker regions of 2 nt (v0.1) and 3 nt (v0.2) were tested. Weaving was introduced into the structure and staple strands were added to constrain the end of the scaffold loops (FIG. 15A). As seen in FIG. 16B, there was no assembly without the queen in any of the groups and under any of the salt concentration conditions.

Example 4

This Example demonstrates assembly of 6-helix bundle DNA nanorod drones into a crisscross structure via nucleation by a gridiron queen (FIGS. 19A-19D). The gridiron queen has 16 cells, each to which may bind a drone using 5 cooperative plug-socket binding sites. Shown here is site-specific binding of two 440 nm long drones (FIG. 19A), one 250 nm long drone (FIG. 19B), and sixteen 250 nm long drones (FIG. 19C). This example shows binding of drones to the gridiron queen using a 10 bp plug-socket. The agarose gel image in FIG. 19D shows that the queen in the reaction is completely bound by drones, when stoichiometric excess of drones is present. Additionally, functionality of the plug-socket binding system (FIG. 18) is shown with the TEM micrographs of these assemblies and kinetics data (FIGS. 19A-D and FIG. 20).

The gridiron queen and 6-helix bundle drones were conceptualized and then designed using the caDNAno design tool (see FIGS. 16A-16C (and data not shown) for the queen, FIGS. 17A-17B (and data not shown) for the drones). Staple sequences designed in caDNAno were ordered commercially and folded with M13 phage scaffold DNA over the following conditions: drones in 6 mM MgCl₂, (90° C./2 mins, 60-50° C./18 hrs); queen in 8 mM MgCl₂, ({(94° C.-86° C.) in 4° C./5 min steps}; {(85° C.-70° C.) in 1° C./5 minute steps}; {(70° C. to 40° C.) in 1° C./15 minutes steps}; {(40° C. to 25° C.) in 1° C./10 minute steps}). The scaffold for the gridiron queen was comprised of a 8634 base genome from M13 phage, and staple DNA sequences were determined by caDNAno. Binding sequences for drones were manually appended to the 3′ ends of the staple DNA to bind drones in the desired orientation. The 250 nm and 440 nm 6 hb drones were also designed in caDNAno. The scaffold DNA was comprised of either the 8064 base genome from M13 phage (for the 440 nm drone), or a custom 3825 base sequence derived from M13 phage (for the 250 nm drone). Staple DNA sequences were determined by caDNAno and purchased commercially. Scaffold sections for the sockets and plug sequences were customized to determine the orientation and final location of sub-components in assembled structures. The 5′ ends of a subset of the staples are truncated to free scaffold so that it could act as a socket to bind plugs. The 3′ ends of another subset of staples were appended with plug DNA sequences so that they could interact with other worker subcomponents.

The folded structures were separated from excess folding staples using agarose gel electrophoresis and bands containing the structure of interest were purified from the agarose gel matrix. The purified structures were placed onto carbon grids, stained with 2% uranyl formate, and analyzed by TEM to validate assembly of the correct structure (see FIG. 16C for the queen and FIG. 17B for the drones).

The purified sub-components were assembled into crisscross formation using the following conditions: 0.1 or 0.01 nM queen, 1 nM drones, 30 mM MgCl₂, 45 mM Tris-borate, 1 mM EDTA, and 0.01% Tween-20; incubation at 50° Celsius for 8-24 hours. Assembly reactions were analyzed using gel electrophoresis and TEM, as shown in FIGS. 19A-19D. Kinetics of binding between the drone and queen are shown in FIG. 20.

Example 5

A similar system was built using single-stranded DNA (ssDNA) instead of 6 helix bundles, referred to as “crisscross DNA slats” (short: “DNA slats”). A schematic of the base unit is shown in FIGS. 21A-21B. The 21 nucleotide (nt) long oligonucleotide per DNA slat shown in FIGS. 21A-21B allows the 4 by 4 DNA slats array to retains the correct 10.5 bases/turn. The length of the DNA slats can be expanded by repeats of 21 nt, for example, achieving larger structures. FIG. 21A shows an abstraction of the DNA slats architecture and a matrix with the number of base pairs (bp) per binding site at each position. The alternation of 6 bp and 5 bp is used to retain the correct helicity and approximately same binding energy per DNA slat. An exemplary DNA slat strand with 16 binding sites (84 nt) is shown below:

(SEQ ID NO: 684) TGGTTCTGGAGTTTTACTCGGGACACTTCAGCGTAATATCGGAAGCAG GCACTTTGAAACCTATAAGTCCTGACTATTAATAAC.

FIG. 21B shows a 3D rendering from the front and cross-section of the DNA slats architecture. The strands weave over and under each other. The DNA slats can reliably tile the ssDNA overhangs (from the M13 scaffold) of different DNA-Origami queens. FIGS. 23A-24B both show examples of ssDNA scaffold being tiled upon and the addition of DNA slats. The flat DNA-Origami queen shown in FIG. 23A is folded through a 2 minute 90° C. denaturing period following an 18-hour ramp from 55° C.-50° C. with 6 mM MgCl₂. The barrel DNA-Origami queen shown in FIG. 24A is folded by a 15 minute 80° C. denaturing period following an 18-hour ramp from 60° C.-25° C. with 8 mM MgCl₂. The assembly process of DNA slats with the queen is shown in FIG. 22. Once the queen is folded the crude reaction queen is mixed with the DNA slats, assembly conditions can be tuned by varying the concentrations of DNA slats (100 nM-1000 nM), salt (5 mM-30 mM MgCl₂ and 0-1 M NaCl), and the temperature of assembly (4° C.-55° C.). By altering the assembly conditions such as MgCl₂ and the DNA slat concentration the kinetics of the assembly process can be influenced.

Example 6

This Example shows how extending the DNA slats to create more binding sites facilitates polymerization seeded on the queen. FIG. 25 shows both flat and barrel queen first tiled (as shown in FIGS. 23A-23B and FIGS. 24A-24B) and with extended DNA slats seeding the next generation of DNA slats to bind and eventually grow into micron sized structures. The queen nucleating site determines the shape of the subsequently grown structure. FIGS. 26A and 26B show the flat queen assembled in two types of terminal extensions. FIGS. 26C-26E show the flat queen with one, two, and three domain extensions. Samples shown in FIGS. 26A-26E were prepared using crude flat queen reaction (˜1 nM), DNA slats (1000 nM/strand), and 15 mM MgCl₂ at 50 □ for 2 hours. FIGS. 30A-30B show a staggered design of DNA slats that bind to the flat queen, growing a ribbon like sheet. The subsequent formation of ribbons is shown in FIG. 31. Control reactions, without the flat queen, showed no assembly of the DNA slats after 18 hours of running the reaction. Samples shown in FIG. 31 were prepared using crude flat queen reaction (˜9 nM), DNA slats (7500 nM/strand), and 14 mM MgCl₂ at 50 □ for ˜66 hours.

Example 7

Through the use of the barrel DNA-Origami queen multiple guest-ring catenane systems can be produced in a one-pot reaction. FIGS. 27A-27C show that by folding the barrel queen, a multiple guest-ring catenane system can be achieved through the addition of DNA slats. In order to catenate the loops two DNA slats are needed. A close up view of the DNA slats weaving and catenating the ssDNA M13 scaffold loops is shown in FIG. 27C. By ligating the two DNA slats on one end, a single DNA slat is created that captures all eight loops. A 3D rendering of the purple DNA slat capturing eight loops is shown in FIG. 27D. FIG. 24 shows the addition of 64 slats, which can simply be reduced, depending on size of the size and number of guest rings. Using the barrel queen with the DNA slats achieves a high yield in a one-pot reaction. The barrel queen can subsequently be transformed into an ultrasensitive biosensor, by coupling a biomolecule detection system to the DNA slats (see, e.g., FIG. 31). Through the integration of proofreading steps, the analyte presence can be transferred into the open or closed state of the purple DNA slat. FIGS. 28A-28B show that without a biomolecule present, the queen falls apart (open DNA slats) and no nucleation of DNA slat mediated growth can occur. The presence of a biomolecule, however, keeps the DNA slats intact and holds the queen structure together, which can then trigger the growth of micron sized tubes, for example, that can subsequently be detected using low-cost optical instruments.

TABLE 1 Exemplary Gridiron Queen Staple Sequences SEQ ID Sequence Comment NO: ATCTGAACTCGCTACGGCGGGGGGAGCCCC 20170216_cc6hb_v3_queen, c0, h21, p0, control,   1 CGATTTAGAGCT cyan, start 0[94], end 21[94], 42 mer CATTGCTGATACCGTTTAGCTAACAAACATC 20170216_cc6hb_v3_queen, c0, h20, p1, control,   2 AAGAAAACAAA cyan, start 1[95], end 20[95], 42 mer GATACTTGCCCTCTCTGTACATAATTAATTT 20170216_cc6hb_v3_queen, c0, h19, p2, control,   3 TCCCTTAGAAT cyan, start 0[178], end 19[94], 42 mer GATTGGGCGTTATCAATGTTGTTTTGTCACA 20170216_cc6hb_v3_queen, c0, h18, p3, control,   4 ATCAATAGAAA cyan, start 1[179], end 18[95], 42 mer TCTAATGAAGACAAATCCCCACGTCACCGA 20170216_cc6hb_v3_queen, c0, h17, p4, control,   5 CTTGAGCCATTT cyan, start 0[262], end 17[94], 42 mer AAACATCGGGTTGAGTATTATGTGGCGAGA 20170216_cc6hb_v3_queen, c1, h21, p4, control,   6 AAGGAAGGGAAG cyan, start 1[53], end 21[136], 42 mer CGCTGGCATTCGCATCAAAGGCGAATTATT 20170216_cc6hb_v3_queen, c1, h20, p3, control,   7 CATTTCAATTAC cyan, start 2[136], end 20[137], 42 mer AGTTTATAAATGAGTATCAATTTAGATTAAG 20170216_cc6hb_v3_queen, c1, h19, p2, control,   8 ACGCTGAGAAG cyan, start 1[137], end 19[136], 42 mer TATCGACATCATTACGCATCGCAACATATA 20170216_cc6hb_v3_queen, c1, h18, p1, control,   9 AAAGAAACGCAA cyan, start 2[220], end 18[137], 42 mer CCATGCAGACATCACGAAGGTCACCAGTAG 20170216_cc6hb_v3_queen, c1, h17, p0, control,  10 CACCATTACCAT cyan, start 1[221], end 17[136], 42 mer AAGATAACGCTTGTGAAAATGAGGGCGCTG 20170216_cc6hb_v3_queen, c2, h21, p0, control,  11 GCAAGTGTAGCG cyan, start 2[94], end 21[178], 42 mer GCTAACAGTAGGGAAACTGCGGCCTGATTG 20170216_cc6hb_v3_queen, c2, h20, p1, control,  12 CTTTGAATACCA cyan, start 3[95], end 20[179], 42 mer ATGGGTTCAGGATGCAGGTGAAATCATAGG 20170216_cc6hb_v3_queen, c2, h19, p2, control,  13 TCTGAGAGACTA cyan, start 2[178], end 19[178], 42 mer CTCGGATGGGAGTAAGCGTATGCAGTATGT 20170216_cc6hb_v3_queen, c2, h18, p3, control,  14 TAGCAAACGTAG cyan, start 3[179], end 18[179], 42 mer AGAGTTTCTGCGGCAGTTAATCAATGAAAC 20170216_cc6hb_v3_queen, c2, h17, p4, control,  15 CATCGATAGCAG cyan, start 2[262], end 17[178], 42 mer GCAATACATCAAACGCCGCGAACACCCGCC 20170216_cc6hb_v3_queen, c3, h21, p4, control,  16 GCGCTTAATGCG cyan, start 3[53], end 21[220], 42 mer TCAGGCACTGCGTGAAGCGGCAGTAACAGT 20170216_cc6hb_v3_queen, c3, h20, p3, control,  17 ACCTTTTACATC cyan, start 4[136], end 20[221], 42 mer ATCAAAACTCAACGAGCAGCGGTTGGGTTA 20170216_cc6hb_v3_queen, c3, h19, p2, control,  18 TATAACTATATG cyan, start 3[137], end 19[220], 42 mer AGGGTTGTCGGACTTGTGCAAGGAATACCC 20170216_cc6hb_v3_queen, c3, h18, p1, control,  19 AAAAGAACTGGC cyan, start 4[220], end 18[221], 42 mer AGTCCGTGAAGACGGAAACCAAATCAAGTT 20170216_cc6hb_v3_queen, c3, h17, p0, control,  20 TGCCTTTAGCGT cyan, start 3[221], end 17[220], 42 mer CTGGGGATTTGACGCAGACCTGGTTGCTTTG 20170216_cc6hb_v3_queen, c4, h21, p0, control,  21 ACGAGCACGTA cyan, start 4[94], end 21[262], 42 mer TTTTCCCAGTCACGACGTTGTGAAATTGCGT 20170216_cc6hb_v3_queen, c4, h20, p1, control,  22 AGATTTTCAGG cyan, start 5[95], end 20[263], 42 mer TTATCAGTAAACAGAGAGGTTTCGCAAGAC 20170216_cc6hb_v3_queen, c4, h19, p2, control,  23 AAAGAACGCGAG cyan, start 4[178], end 19[262], 42 mer TCAGGGATTAATGAAAGATGGAACAAAGTT 20170216_cc6hb_v3_queen, c4, h18, p3, control,  24 ACCAGAAGGAAA cyan, start 5[179], end 18[263], 42 mer AGTGTGGCGATCCGATAGATGCGGCATTTT 20170216_cc6hb_v3_queen, c4, h17, p4, control,  25 CGGTCATAGCCC cyan, start 4[262], end 17[262], 42 mer GGGGGATGTGCTGCAAGGCGAATCAGAGCG 20170216_cc6hb_v3_queen, c5, h21, p4, control,  26 GGAGCTAAACAG cyan, start 5[53], end 21[304], 42 mer AGCCAGCTTTCCGGCACCGCTACCTACCATA 20170216_cc6hb_v3_queen, c5, h20, p3, control,  27 TCAAAATTATT cyan, start 6[136], end 20[305], 42 mer CTTTATTATTCGCATTCACCCTAGTTAATTTC 20170216_cc6hb_v3_queen, c5, h19, p2, control,  28 ATCTTCTGAC cyan, start 5[137], end 19[304], 42 mer TTGGTGTAGATGGGCGCATCGATCTTACCG 20170216_cc6hb_v3_queen, c5, h18, p1, control,  29 AAGCCCTTTTTA cyan, start 6[220], end 18[305], 42 mer CAGAAATAGAAGAATTACAGCTTTCATAAT 20170216_cc6hb_v3_queen, c5, h17, p0, control,  30 CAAAATCACCGG cyan, start 5[221], end 17[304], 42 mer AAGCGCCATTCGCCATTCAGGAGACAGGAA 20170216_cc6hb_v3_queen, c6, h21, p0, control,  31 CGGTACGCCAGA cyan, start 6[94], end 21[346], 42 mer TCAGAAAAGCCCCAAAAACAGCTGATTGTT 20170216_cc6hb_v3_queen, c6, h20, p1, control,  32 TGGATTATACTT cyan, start 7[95], end 20[347], 42 mer GAGGGGACGACGACAGTATCGACCGACCGT 20170216_cc6hb_v3_queen, c6, h19, p2, control,  33 GTGATAAATAAG cyan, start 6[178], end 19[346], 42 mer TTTTTGTTAAATCAGCTCATTAGCCCAATAA 20170216_cc6hb_v3_queen, c6, h18, p3, control,  34 TAAGAGCAAGA cyan, start 7[179], end 18[347], 42 mer GTGGGAACAAACGGCGGATTGCGCCTCCCT 20170216_cc6hb_v3_queen, c6, h17, p4, control,  35 CAGAGCCGCCAC cyan, start 6[262], end 17[346], 42 mer TCGTAAAACTAGCATGTCAATATCAGTGAG 20170216_cc6hb_v3_queen, c7, h21, p4, control,  36 GCCACCGAGTAA cyan, start 7[53], end 21[388], 42 mer ATGATATTCAACCGTTCTAGCATATTCCTGA 20170216_cc6hb_v3_queen, c7, h20, p3, control,  37 TTATCAGATGA cyan, start 8[136], end 20[389], 42 mer TTAAATTGTAAACGTTAATATCGGAATCATA 20170216_cc6hb_v3_queen, c7, h19, p2, control,  38 ATTACTAGAAA cyan, start 7[137], end 19[388], 42 mer TATTTTAAATGCAATGCCTGATGAGCGCTAA 20170216_cc6hb_v3_queen, c7, h18, p1, control,  39 TATCAGAGAGA cyan, start 8[220], end 18[389], 42 mer TCAAAAATAATTCGCGTCTGGAGCCACCAC 20170216_cc6hb_v3_queen, c7, h17, p0, control,  40 CCTCAGAGCCGC cyan, start 7[221], end 17[388], 42 mer GGTAGCTATTTTTGAGAGATCATTAACCGTT 20170216_cc6hb_v3_queen, c8, h21, p0, control,  41 GTAGCAATACT cyan, start 8[94], end 21[430], 42 mer ATGGTCAATAACCTGTTTAGCTTGCGGAAC 20170216_cc6hb_v3_queen, c8, h20, p1, control,  42 AAAGAAACCACC cyan, start 9[95], end 20[431], 42 mer AAAAGGGTGAGAAAGGCCGGACGTTATACA 20170216_cc6hb_v3_queen, c8, h19, p2, control,  43 AATTCTTACCAG cyan, start 8[178], end 19[430], 42 mer AACATCCAATAAATCATACAGGGGAGAATT 20170216_cc6hb_v3_queen, c8, h18, p3, control,  44 AACTGAACACCC cyan, start 9[179], end 18[431], 42 mer CTTTATTTCAACGCAAGGATACGCCGCCAG 20170216_cc6hb_v3_queen, c8, h17, p4, control,  45 CATTGACAGGAG cyan, start 8[262], end 17[430], 42 mer CGAACGAGTAGATTTAGTTTGACTTGCCTGA 20170216_cc6hb_v3_queen, c9, h21, p4, control,  46 GTAGAAGAACT cyan, start 9[53], end 21[472], 42 mer CATTTTTGCGGATGGCTTAGACCGAACGTTA 20170216_cc6hb_v3_queen, c9, h20, p3, control,  47 TTAATTTTAAA cyan, start 10[136], end 20[473], 42 mer AGCTGAAAAGGTGGCATCAATTAGGGCTTA 20170216_cc6hb_v3_queen, c9, h19, p2, control,  48 ATTGAGAATCGC cyan, start 9[137], end 19[472], 42 mer AGCTTCAAAGCGAACCAGACCTTTACAGAG 20170216_cc6hb_v3_queen, c9, h18, p1, control,  49 AGAATAACATAA cyan, start 10[220], end 18[473], 42 mer ATTAAGCAATAAAGCCTCAGAGGCCTTGAT 20170216_cc6hb_v3_queen, c9, h17, p0, control,  50 ATTCACAAACAA cyan, start 9[221], end 17[472], 42 mer CTGTAGCTCAACATGTTTTAAAATATCCAGA 20170216_cc6hb_v3_queen, c10, h21, p0, control,  51 ACAATATTACC cyan, start 10[94], end 21[514], 42 mer GGCTTTTGCAAAAGAAGTTTTAGACTTTACA 20170216_cc6hb_v3_queen, c10, h20, p1, control,  52 AACAATTCGAC cyan, start 11[95], end 20[515], 42 mer AGGATTAGAGAGTACCTTTAAGTAATTTAG 20170216_cc6hb_v3_queen, c10, h19, p2, control,  53 GCAGAGGCATTT cyan, start 10[178], end 19[514], 42 mer AATATTCATTGAATCCCCCTCGAAACGATTT 20170216_cc6hb_v3_queen, c10, h18, p3, control,  54 TTTGTTTAACG cyan, start 11[179], end 18[515], 42 mer AAGAGGAAGCCCGAAAGACTTAATGGAAA 20170216_cc6hb_v3_queen, c10, h17, p4, control,  55 GCGCAGTCTCTGA cyan, start 10[262], end 17[514], 42 mer ACCCTCGTTTACCAGACGACGAACGCTCAT 20170216_cc6hb_v3_queen, c11, h21, p4, control,  56 GGAAATACCTAC cyan, start 11[53], end 21[556], 42 mer TAACGGAACAACATTATTACAAGAGCCGTC 20170216_cc6hb_v3_queen, c11, h20, p3, control,  57 AATAGATAATAC cyan, start 12[136], end 20[557], 42 mer ATGTTTAGACTGGATAGCGTCATAAAGTAC 20170216_cc6hb_v3_queen, c11, h19, p2, control,  58 CGACAAAAGGTA cyan, start 11[137], end 19[556], 42 mer TGAATTACCTTATGCGATTTTTTACAAAATA 20170216_cc6hb_v3_queen, c11, h18, p1, control,  59 AACAGCCATAT cyan, start 12[220], end 18[557], 42 mer AAACGAGAATGACCATAAATCCATACATGG 20170216_cc6hb_v3_queen, c11, h17, p0, control,  60 CTTTTGATGATA cyan, start 11[221], end 17[556], 42 mer AGATTTAGGAATACCACATTCAAATGGATT 20170216_cc6hb_v3_queen, c12, h21, p0, control,  61 ATTTACATTGGC cyan, start 12[94], end 21[598], 42 mer CGAGGCGCAGACGGTCAATCAGTTATCTAA 20170216_cc6hb_v3_queen, c12, h20, p1, control,  62 AATATCTTTAGG cyan, start 13[95], end 20[599], 42 mer GTCAGGACGTTGGGAAGAAAAGACAATAA 20170216_cc6hb_v3_queen, c12, h19, p2, control,  63 ACAACATGTTCAG cyan, start 12[178], end 19[598], 42 mer AGGCTGGCTGACCTTCATCAATACCAACGC 20170216_cc6hb_v3_queen, c12, h18, p3, control,  64 TAACGAGCGTCT cyan, start 13[179], end 18[599], 42 mer TAAATTGGGCTTGAGATGGTTTTTTAACGGG 20170216_cc6hb_v3_queen, c12, h17, p4, control,  65 GTCAGTGCCTT cyan, start 12[262], end 17[598], 42 mer TGTGTCGAAATCCGCGACCTGAGTAATAAA 20170216_cc6hb_v3_queen, c13, h21, p4, control,  66 AGGGACATTCTG cyan, start 13[53], end 21[640], 42 mer TACGAAGGCACCAACCTAAAACTGGTCAGT 20170216_cc6hb_v3_queen, c13, h20, p3, control,  67 TGGCAAATCAAC cyan, start 14[136], end 20[641], 42 mer CTTTGAAAGAGGACAGATGAATATCAACAA 20170216_cc6hb_v3_queen, c13, h19, p2, control,  68 TAGATAAGTCCT cyan, start 13[137], end 19[640], 42 mer GTAGCAACGGCTACAGAGGCTTAGTTGCTA 20170216_cc6hb_v3_queen, c13, h18, p1, control,  69 TTTTGCACCCAG cyan, start 14[220], end 18[641], 42 mer GATATTCATTACCCAAATCAACAGTTAATGC 20170216_cc6hb_v3_queen, c13, h17, p0, control,  70 CCCCTGCCTAT cyan, start 13[221], end 17[640], 42 mer CTAAAACACTCATCTTTGACCCTGACCTGAA 20170216_cc6hb_v3_queen, c14, h21, p0, control,  71 AGCGTAAGAAT cyan, start 14[94], end 21[682], 42 mer CGAATAATAATTTTTTCACGTATCACCTTGC 20170216_cc6hb_v3_queen, c14, h20, p1, control,  72 TGAACCTCAAA cyan, start 15[95], end 20[683], 42 mer ATGAGGAAGTTTCCATTAAACATCCTAATTT 20170216_cc6hb_v3_queen, c14, h19, p2, control,  73 ACGAGCATGTA cyan, start 14[178], end 19[682], 42 mer TTCGAGGTGAATTTCTTAAACACCTCCCGAC 20170216_cc6hb_v3_queen, c14, h18, p3, control,  74 TTGCGGGAGGT cyan, start 15[179], end 18[683], 42 mer GGATCGTCACCCTCAGCAGCGACATGAAAG 20170216_cc6hb_v3_queen, c14, h17, p4, control,  75 TATTAAGAGGCT cyan, start 14[262], end 17[682], 42 mer TTTCAGCGGAGTGAGAATAGATGAATGGCT 20170216_cc6hb_v3_queen, c15, h21, p4, control,  76 ATTAGTCTTTAA cyan, start 15[53], end 21[724], 42 mer CTACAACGCCTGTAGCATTCCAGTGCCACG 20170216_cc6hb_v3_queen, c15, h20, p3, control,  77 CTGAGAGCCAGC cyan, start 16[136], end 20[725], 42 mer CTCCAAAAGGAGCCTTTAATTGTCTTTCCTT 20170216_cc6hb_v3_queen, c15, h19, p2, control,  78 ATCATTCCAAG cyan, start 15[137], end 19[724], 42 mer CAGAGCCACCACCCTCATTTTAAGGCTTATC 20170216_cc6hb_v3_queen, c15, h18, p1, control,  79 CGGTATTCTAA cyan, start 16[220], end 18[725], 42 mer CCGACAATGACAACAACCATCTAGGATTAG 20170216_cc6hb_v3_queen, c15, h17, p0, control,  80 CGGGGTTTTGCT cyan, start 15[221], end 17[724], 42 mer TCGTAAAACTAGCATGTCAATATCAGTGAG 20170216_cc6hb_v3_queen, c7, h21, p4, 7 bp plug,  81 GCCACCGAGTAAGAAAAAC cyan, start 7[53], end 21[388], 49 mer ATGATATTCAACCGTTCTAGCATATTCCTGA 20170216_cc6hb_v3_queen, c7, h20, p3, 7 bp plug,  82 TTATCAGATGAAGAGTCC cyan, start 8[136], end 20[389], 49 mer TTAAATTGTAAACGTTAATATCGGAATCATA 20170216_cc6hb_v3_queen, c7, h19, p2, 7 bp plug,  83 ATTACTAGAAAAATAGCC cyan, start 7[137], end 19[388], 49 mer TATTTTAAATGCAATGCCTGATGAGCGCTAA 20170216_cc6hb_v3_queen, c7, h18, p1, 7 bp plug,  84 TATCAGAGAGAATGGTGG cyan, start 8[220], end 18[389], 49 mer TCAAAAATAATTCGCGTCTGGAGCCACCAC 20170216_cc6hb_v3_queen, c7, h17, p0, 7 bp plug,  85 CCTCAGAGCCGCCGGTCCA cyan, start 7[221], end 17[388], 49 mer GGTAGCTATTTTTGAGAGATCATTAACCGTT 20170216_cc6hb_v3_queen, c8, h21, p0, 7 bp plug,  86 GTAGCAATACTCGGTCCA cyan, start 8[94], end 21[430], 49 mer ATGGTCAATAACCTGTTTAGCTTGCGGAAC 20170216_cc6hb_v3_queen, c8, h20, p1, 7 bp plug,  87 AAAGAAACCACCATGGTGG cyan, start 9[95], end 20[431], 49 mer AAAAGGGTGAGAAAGGCCGGACGTTATACA 20170216_cc6hb_v3_queen, c8, h19, p2, 7 bp plug,  88 AATTCTTACCAGAATAGCC cyan, start 8[178], end 19[430], 49 mer AACATCCAATAAATCATACAGGGGAGAATT 20170216_cc6hb_v3_queen, c8, h18, p3, 7 bp plug,  89 AACTGAACACCCAGAGTCC cyan, start 9[179], end 18[431], 49 mer CTTTATTTCAACGCAAGGATACGCCGCCAG 20170216_cc6hb_v3_queen, c8, h17, p4, 7 bp plug,  90 CATTGACAGGAGGAAAAAC cyan, start 8[262], end 17[430], 49 mer TCGTAAAACTAGCATGTCAATATCAGTGAG 20170216_cc6hb_v3_queen, c7, h21, p4, 10 bp plug,  91 GCCACCGAGTAAGAAAAACCGT cyan, start 7[53], end 21[388], 52 mer ATGATATTCAACCGTTCTAGCATATTCCTGA 20170216_cc6hb_v3_queen, c7, h20, p3, 10 bp plug,  92 TTATCAGATGAAGAGTCCACT cyan, start 8[136], end 20[389], 52 mer TTAAATTGTAAACGTTAATATCGGAATCATA 20170216_cc6hb_v3_queen, c7, h19, p2, 10 bp plug,  93 ATTACTAGAAAAATAGCCCGA cyan, start 7[137], end 19[388], 52 mer TATTTTAAATGCAATGCCTGATGAGCGCTAA 20170216_cc6hb_v3_queen, c7, h18, p1, 10 bp plug,  94 TATCAGAGAGAATGGTGGTTC cyan, start 8[220], end 18[389], 52 mer TCAAAAATAATTCGCGTCTGGAGCCACCAC 20170216_cc6hb_v3_queen, c7, h17, p0, 10 bp plug,  95 CCTCAGAGCCGCCGGTCCACGC cyan, start 7[221], end 17[388], 52 mer GGTAGCTATTTTTGAGAGATCATTAACCGTT 20170216_cc6hb_v3_queen, c8, h21, p0, 10 bp plug,  96 GTAGCAATACTCGGTCCACGC cyan, start 8[94], end 21[430], 52 mer ATGGTCAATAACCTGTTTAGCTTGCGGAAC 20170216_cc6hb_v3_queen, c8, h20, p1, 10 bp plug,  97 AAAGAAACCACCATGGTGGTTC cyan, start 9[95], end 20[431], 52 mer AAAAGGGTGAGAAAGGCCGGACGTTATACA 20170216_cc6hb_v3_queen, c8, h19, p2, 10 bp plug,  98 AATTCTTACCAGAATAGCCCGA cyan, start 8[178], end 19[430], 52 mer AACATCCAATAAATCATACAGGGGAGAATT 20170216_cc6hb_v3_queen, c8, h18, p3, 10 bp plug,  99 AACTGAACACCCAGAGTCCACT cyan, start 9[179], end 18[431], 52 mer CTTTATTTCAACGCAAGGATACGCCGCCAG 20170216_cc6hb_v3_queen, c8, h17, p4, 10 bp plug, 100 CATTGACAGGAGGAAAAACCGT cyan, start 8[262], end 17[430], 52 mer CAATATTACATAACAATCCTCCATTTGAATT 20170216_cc6hb_v3_queen, edge, na, na, na, black, 101 ACCTTTTTTAA start 0[136], end 20[53], 42 mer ACTGATACCGTGCAAAATTATCAAAGACAA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 102 AAGGGCGACATT start 0[220], end 18[53], 42 mer CGTAACGATCTAAAGTTTTGTAACATCGCCA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 103 TTAAAAATACC start 16[94], end 21[766], 42 mer GAACCCATGTACCGTAACACTCGCACTCAT 20170216_cc6hb_v3_queen, edge, na, na, na, black, 104 CGAGAACAAGCA start 16[178], end 19[766], 42 mer TACCGCCACCCTCAGAACCGCCGTCGAGAG 20170216_cc6hb_v3_queen, edge, na, na, na, black, 105 GGTTGATATAAG start 16[262], end 17[766], 42 mer TCATTAAAGGTGAATTATCACTTCTGCAATG 20170216_cc6hb_v3_queen, edge, na, na, na, black, 106 TGCGAGAAATG start 17[53], end 0[221], 42 mer GGGAATTAGAGCCAGCAAAATGTTTATGTA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 107 GATGAAGGTATA start 17[95], end 1[262], 42 mer CACCGTAATCAGTAGCGACAGGTTTCTTGTT 20170216_cc6hb_v3_queen, edge, na, na, na, black, 108 GTTCGCCATCC start 17[179], end 3[262], 42 mer CCTTATTAGCGTTTGCCATCTGCAACACAGC 20170216_cc6hb_v3_queen, edge, na, na, na, black, 109 AATAAAAATGC start 17[263], end 5[262], 42 mer CCTCAGAACCGCCACCCTCAGCCTTCCTGTA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 110 GCCAGCTTTCA start 17[347], end 7[262], 42 mer GTTGAGGCAGGTCAGACGATTGCATAAAGC 20170216_cc6hb_v3_queen, edge, na, na, na, black, 111 TAAATCGGTTGT start 17[431], end 9[262], 42 mer ATTTACCGTTCCAGTAAGCGTAAAAATCAG 20170216_cc6hb_v3_queen, edge, na, na, na, black, 112 GTCTTTACCCTG start 17[515], end 11[262], 42 mer GAGTAACAGTGCCCGTATAAACGTAACAAA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 113 GCTGCTCATTCA start 17[599], end 13[262], 42 mer GAGACTCCTCAAGAGAAGGATGCCCACGCA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 114 TAACCGATATAT start 17[683], end 15[262], 42 mer TAGCAAGCAAATCAGATATAGCAGGGATAG 20170216_cc6hb_v3_queen, edge, na, na, na, black, 115 CAAGCCCAATAG start 18[766], end 16[179], 42 mer GCTTCTGTAAATCGTCGCTATAAACATATAG 20170216_cc6hb_v3_queen, edge, na, na, na, black, 116 ATGATTAAACC start 19[53], end 0[137], 42 mer AGTATTAACACCGCCTGCAACACAGACAGC 20170216_cc6hb_v3_queen, edge, na, na, na, black, 117 CCTCATAGTTAG start 20[766], end 16[95], 42 mer GCACTAAATCGGAACCCTAAATTTTGTTTTA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 118 TGGAGATGATA start 21[53], end 0[53], 42 mer AAAGCGAAAGGAGCGGGCGCTCTGAATTTC 20170216_cc6hb_v3_queen, edge, na, na, na, black, 119 GCGTCGTCTTCA start 21[137], end 2[53], 42 mer CCGCTACAGGGCGCGTACTATTTTCCATGAA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 120 TTGGTAACACC start 21[221], end 4[53], 42 mer GAGGCCGATTAAAGGGATTTTCTGCGCAAC 20170216_cc6hb_v3_queen, edge, na, na, na, black, 121 TGTTGGGAAGGG start 21[305], end 6[53], 42 mer AAGAGTCTGTCCATCACGCAATACAAAGGC 20170216_cc6hb_v3_queen, edge, na, na, na, black, 122 TATCAGGTCATT start 21[389], end 8[53], 42 mer CAAACTATCGGCCTTGCTGGTATATGCAACT 20170216_cc6hb_v3_queen, edge, na, na, na, black, 123 AAAGTACGGTG start 21[473], end 10[53], 42 mer ATTTTGACGCTCAATCGTCTGAACTAATGCA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 124 GATACATAACG start 21[557], end 12[53], 42 mer GCCAACAGAGATAGAACCCTTCCCAGCGAT 20170216_cc6hb_v3_queen, edge, na, na, na, black, 125 TATACCAAGCGC start 21[641], end 14[53], 42 mer TGCGCGAACTGATAGCCCTAACGTCTTTCCA 20170216_cc6hb_v3_queen, edge, na, na, na, black, 126 GACGTTAGTAA start 21[725], end 16[53], 42 mer CAAAGGGCGAAAAACCAACAGCTGATTGCC 20170216_cc6hb_v3_queen, reference sheet, na, na, 127 CTGCGCCAGG na, puke green, start 22[79], end 24[72], 40 mer AGTCCACTATTAAAGAAGAGAGTTGCAGCA 20170216_cc6hb_v3_queen, reference sheet, na, na, 128 AGCAACGCGC na, puke green, start 22[111], end 24[104], 40 mer TAGGGTTGAGTGTTGTGCCCCAGCAGGCGA 20170216_cc6hb_v3_queen, reference sheet, na, na, 129 AAACCTGTCG na, puke green, start 22[143], end 24[136], 40 mer AATCCCTTATAAATCAGTTCCGAAATCGGC 20170216_cc6hb_v3_queen, reference sheet, na, na, 130 AA na, puke green, start 22[175], end 23[175], 32 mer GTGAGACGGGCGTCTATCA 20170216_cc6hb_v3_queen, reference sheet, na, na, 131 na, puke green, start 23[53], end 22[56], 19 mer GTGGTTTTTGTTTCCTGTGTGAAA 20170216_cc6hb_v3_queen, reference sheet, na, na, 132 na, puke green, start 24[71], end 25[79], 24 mer CGTATTGGTCACCGCCTGGCCCTGACGTGG 20170216_cc6hb_v3_queen, reference sheet, na, na, 133 ACTCCAACGT na, puke green, start 24[87], end 22[80], 40 mer GGGGAGAGATTCCACACAACATAC 20170216_cc6hb_v3_queen, reference sheet, na, na, 134 na, puke green, start 24[103], end 25[111], 24 mer GAATCGGCCGGTCCACGCTGGTTTTCCAGTT 20170216_cc6hb_v3_queen, reference sheet, na, na, 135 TGGAACAAG na, puke green, start 24[119], end 22[112], 40 mer TGCCAGCTGTGTAAAGCCTGGGGT 20170216_cc6hb_v3_queen, reference sheet, na, na, 136 na, puke green, start 24[135], end 25[143], 24 mer GTCGGGAAATCCTGTTTGATGGTGAAAGAA 20170216_cc6hb_v3_queen, reference sheet, na, na, 137 TAGCCCGAGA na, puke green, start 24[151], end 22[144], 40 mer GTTGCGCTCACTGCCCAACTCACATTAATTG 20170216_cc6hb_v3_queen, reference sheet, na, na, 138 C na, puke green, start 24[175], end 25[175], 32 mer GTCATAGCTCTTTTCACCA 20170216_cc6hb_v3_queen, reference sheet, na, na, 139 na, puke green, start 25[56], end 24[53], 19 mer TTGTTATCCGCTCACAGCGGTTTG 20170216_cc6hb_v3_queen, reference sheet, na, na, 140 na, puke green, start 25[80], end 24[88], 24 mer GAGCCGGAAGCATAAAGCATTAAT 20170216_cc6hb_v3_queen, reference sheet, na, na, 141 na, puke green, start 25[112], end 24[120], 24 mer GCCTAATGAGTGAGCTGCTTTCCA 20170216_cc6hb_v3_queen, reference sheet, na, na, 142 na, puke green, start 25[144], end 24[152], 24 mer AAAATACATACATAAAGGTGGCTATTACGG 20170216_cc6hb_v3_queen, cell not used, na, na, 143 GGTTGGAGGTCA na, red, start 18[178], end 2[179], 42 mer TAGCAAGGCCGGAAACGTCACCGAACAAGA 20170216_cc6hb_v3_queen, cell not used, na, na, 144 CCCGTTAGTAAC na, red, start 17[137], end 2[221], 42 mer CAGACTGTAGCGCGTTTTCATAACGAAGAC 20170216_cc6hb_v3_queen, cell not used, na, na, 145 GCCTGGTCGTTC na, red, start 17[221], end 4[221], 42 mer AACCAGAGCCACCACCGGAACACCGTAATG 20170216_cc6hb_v3_queen, cell not used, na, na, 146 GGATAGGTCACG na, red, start 17[305], end 6[221], 42 mer CACCAGAACCACCACCAGAGCAAAATTTTT 20170216_cc6hb_v3_queen, cell not used, na, na, 147 AGAACCCTCATA na, red, start 17[389], end 8[221], 42 mer ATAAATCCTCATTAAAGCCAGCAAATATCG 20170216_cc6hb_v3_queen, cell not used, na, na, 148 CGTTTTAATTCG na, red, start 17[473], end 10[221], 42 mer CAGGAGTGTACTGGTAATAAGTAATTTCAA 20170216_cc6hb_v3_queen, cell not used, na, na, 149 CTTTAATCATTG na, red, start 17[557], end 12[221], 42 mer TTCGGAACCTATTATTCTGAAAAAGACAGC 20170216_cc6hb_v3_queen, cell not used, na, na, 150 ATCGGAACGAGG na, red, start 17[641], end 14[221], 42 mer CAGTACCAGGCGGATAAGTGCCACCCTCAG 20170216_cc6hb_v3_queen, cell not used, na, na, 151 AACCGCCACCCT na, red, start 17[725], end 16[221], 42 mer ATTCATATGGTTTACCAGCGCTATCACGAGT 20170216_cc6hb_v3_queen, cell not used, na, na, 152 ACGGTGGAAAC na, red, start 18[94], end 0[179], 42 mer AGACACCACGGAATAAGTTTATGCAGATCC 20170216_cc6hb_v3_queen, cell not used, na, na, 153 GGTGTCTTGTCT na, red, start 18[136], end 1[220], 42 mer ATGATTAAGACTCCTTATTACTGCTAAACTG 20170216_cc6hb_v3_queen, cell not used, na, na, 154 GAAAGCAACGA na, red, start 18[220], end 3[220], 42 mer CCGAGGAAACGCAATAATAACGTTGCCAGG 20170216_cc6hb_v3_queen, cell not used, na, na, 155 AGGATCTGGAAC na, red, start 18[262], end 4[179], 42 mer AGAAAAGTAAGCAGATAGCCGCAGACATCA 20170216_cc6hb_v3_queen, cell not used, na, na, 156 TTGATTCAGCAT na, red, start 18[304], end 5[220], 42 mer AACAATGAAATAGCAATAGCTTAACCGTGC 20170216_cc6hb_v3_queen, cell not used, na, na, 157 ATCTGCCAGTTT na, red, start 18[346], end 6[179], 42 mer TAACCCACAAGAATTGAGTTATTTTAACCA 20170216_cc6hb_v3_queen, cell not used, na, na, 158 ATAGGAACGCCA na, red, start 18[388], end 7[220], 42 mer TGAACAAAGTCAGAGGGTAATGTAATGTGT 20170216_cc6hb_v3_queen, cell not used, na, na, 159 AGGTAAAGATTC na, red, start 18[430], end 8[179], 42 mer AAACAGGGAAGCGCATTAGACGCAAGGCA 20170216_cc6hb_v3_queen, cell not used, na, na, 160 AAGAATTAGCAAA na, red, start 18[472], end 9[220], 42 mer TCAAAAATGAAAATAGCAGCCGGAAGCAA 20170216_cc6hb_v3_queen, cell not used, na, na, 161 ACTCCAACAGGTC na, red, start 18[514], end 10[179], 42 mer TATTTATCCCAATCCAAATAAAAATGCTTTA 20170216_cc6hb_v3_queen, cell not used, na, na, 162 AACAGTTCAGA na, red, start 18[556], end 11[220], 42 mer TTCCAGAGCCTAATTTGCCAGAAGAACTGG 20170216_cc6hb_v3_queen, cell not used, na, na, 163 CTCATTATACCA na, red, start 18[598], end 12[179], 42 mer CTACAATTTTATCCTGAATCTGAGTAATCTT 20170216_cc6hb_v3_queen, cell not used, na, na, 164 GACAAGAACCG na, red, start 18[640], end 13[220], 42 mer TTTGAAGCCTTAAATCAAGATTTGAGGACT 20170216_cc6hb_v3_queen, cell not used, na, na, 165 AAAGACTTTTTC na, red, start 18[682], end 14[179], 42 mer GAACGCGAGGCGTTTTAGCGAAGCTTGATA 20170216_cc6hb_v3_queen, cell not used, na, na, 166 CCGATAGTTGCG na, red, start 18[724], end 15[220], 42 mer CCTTGAAAACATAGCGATAGCGAGTTAGAG 20170216_cc6hb_v3_queen, cell not used, na, na, 167 TCTGAGCAAAAA na, red, start 19[95], end 1[178], 42 mer AGTCAATAGTGAATTTATCAAGTATCTGCAT 20170216_cc6hb_v3_queen, cell not used, na, na, 168 ATGATGTCTGA na, red, start 19[137], end 2[137], 42 mer CCTTTTTAACCTCCGGCTTAGTGAGTATTAC 20170216_cc6hb_v3_queen, cell not used, na, na, 169 GAAGGTGTTAT na, red, start 19[179], end 3[178], 42 mer TAAATGCTGATGCAAATCCAACGAAGTGAG 20170216_cc6hb_v3_queen, cell not used, na, na, 170 CGAAATTAACTC na, red, start 19[221], end 4[137], 42 mer AAAACTTTTTCAAATATATTTTCATGCGTAT 20170216_cc6hb_v3_queen, cell not used, na, na, 171 TAACCAACAGT na, red, start 19[263], end 5[178], 42 mer CTAAATTTAATGGTTTGAAATGCCTCAGGA 20170216_cc6hb_v3_queen, cell not used, na, na, 172 AGATCGCACTCC na, red, start 19[305], end 6[137], 42 mer GCGTTAAATAAGAATAAACACTTTGTTAAA 20170216_cc6hb_v3_queen, cell not used, na, na, 173 ATTCGCATTAAA na, red, start 19[347], end 7[178], 42 mer AAGCCTGTTTAGTATCATATGGACAGTCAA 20170216_cc6hb_v3_queen, cell not used, na, na, 174 ATCACCATCAAT na, red, start 19[389], end 8[137], 42 mer TATAAAGCCAACGCTCAACAGTCTACTAAT 20170216_cc6hb_v3_queen, cell not used, na, na, 175 AGTAGTAGCATT na, red, start 19[431], end 9[178], 42 mer CATATTTAACAACGCCAACATTTGCTCCTTT 20170216_cc6hb_v3_queen, cell not used, na, na, 176 TGATAAGAGGT na, red, start 19[473], end 10[137], 42 mer TCGAGCCAGTAATAAGAGAATCAATACTGC 20170216_cc6hb_v3_queen, cell not used, na, na, 177 GGAATCGTCATA na, red, start 19[515], end 11[178], 42 mer AAGTAATTCTGTCCAGACGACATCTACGTTA 20170216_cc6hb_v3_queen, cell not used, na, na, 178 ATAAAACGAAC na, red, start 19[557], end 12[137], 42 mer CTAATGCAGAACGCGCCTGTTCGGTGTACA 20170216_cc6hb_v3_queen, cell not used, na, na, 179 GACCAGGCGCAT na, red, start 19[599], end 13[178], 42 mer GAACAAGAAAAATAATATCCCGGGTAAAAT 20170216_cc6hb_v3_queen, cell not used, na, na, 180 ACGTAATGCCAC na, red, start 19[641], end 14[137], 42 mer GAAACCAATCAATAATCGGCTGTATCGGTT 20170216_cc6hb_v3_queen, cell not used, na, na, 181 TATCAGCTTGCT na, red, start 19[683], end 15[178], 42 mer AACGGGTATTAAACCAAGTACGAGTTTCGT 20170216_cc6hb_v3_queen, cell not used, na, na, 182 CACCAGTACAAA na, red, start 19[725], end 16[137], 42 mer ATTAATTACATTTAACAATTTGCACTCGCGG 20170216_cc6hb_v3_queen, cell not used, na, na, 183 GGATTTATTTT na, red, start 20[94], end 0[95], 42 mer CTGAGCAAAAGAAGATGATGAGAAACGAC 20170216_cc6hb_v3_queen, cell not used, na, na, 184 ATACATTGCAAGG na, red, start 20[136], end 1[136], 42 mer AGTTACAAAATCGCGCAGAGGAGAGTGAGA 20170216_cc6hb_v3_queen, cell not used, na, na, 185 TCGGTTTTGTAA na, red, start 20[178], end 2[95], 42 mer GGGAGAAACAATAACGGATTCTGTTGAGCT 20170216_cc6hb_v3_queen, cell not used, na, na, 186 TGAAACAGCAAA na, red, start 20[220], end 3[136], 42 mer TTTAACGTCAGATGAATATACAGAGCAGGC 20170216_cc6hb_v3_queen, cell not used, na, na, 187 AATGCATGACGA na, red, start 20[262], end 4[95], 42 mer TGCACGTAAAACAGAAATAAAAAAACGAC 20170216_cc6hb_v3_queen, cell not used, na, na, 188 GGCCAGTGCCAAG na, red, start 20[304], end 5[136], 42 mer CTGAATAATGGAAGGGTTAGATCTGGTGCC 20170216_cc6hb_v3_queen, cell not used, na, na, 189 GGAAACCAGGCA na, red, start 20[346], end 6[95], 42 mer TGGCAATTCATCAATATAATCGAAGATTGT 20170216_cc6hb_v3_queen, cell not used, na, na, 190 ATAAGCAAATAT na, red, start 20[388], end 7[136], 42 mer AGAAGGAGCGGAATTATCATCTGATAAATT 20170216_cc6hb_v3_queen, cell not used, na, na, 191 AATGCCGGAGAG na, red, start 20[430], end 8[95], 42 mer AGTTTGAGTAACATTATCATTTATATTTTCA 20170216_cc6hb_v3_queen, cell not used, na, na, 192 TTTGGGGCGCG na, red, start 20[472], end 9[136], 42 mer AACTCGTATTAAATCCTTTGCGCTTAATTGC 20170216_cc6hb_v3_queen, cell not used, na, na, 193 TGAATATAATG na, red, start 20[514], end 10[95], 42 mer ATTTGAGGATTTAGAAGTATTGCCAGAGGG 20170216_cc6hb_v3_queen, cell not used, na, na, 194 GGTAATAGTAAA na, red, start 20[556], end 11[136], 42 mer AGCACTAACAACTAATAGATTGGTAGAAAG 20170216_cc6hb_v3_queen, cell not used, na, na, 195 ATTCATCAGTTG na, red, start 20[598], end 12[95], 42 mer AGTTGAAAGGAATTGAGGAAGTAAGGGAA 20170216_cc6hb_v3_queen, cell not used, na, na, 196 CCGAACTGACCAA na, red, start 20[640], end 13[136], 42 mer TATCAAACCCTCAATCAATATCGAAAGAGG 20170216_cc6hb_v3_queen, cell not used, na, na, 197 CAAAAGAATACA na, red, start 20[682], end 14[95], 42 mer AGCAAATGAAAAATCTAAAGCTGAAAATCT 20170216_cc6hb_v3_queen, cell not used, na, na, 198 CCAAAAAAAAGG na, red, start 20[724], end 15[136], 42 mer TGACGGGGAAAGCCGGCGAACCTTACTGTT 20170216_cc6hb_v3_queen, cell not used, na, na, 199 TCTTTACATAAA na, red, start 21[95], end 1[94], 42 mer GTCACGCTGCGCGTAACCACCCCAGGAGAA 20170216_cc6hb_v3_queen, cell not used, na, na, 200 CGAGGATATTGC na, red, start 21[179], end 3[94], 42 mer TAACGTGCTTTCCTCGTTAGATTAAGTTGGG 20170216_cc6hb_v3_queen, cell not used, na, na, 201 TAACGCCAGGG na, red, start 21[263], end 5[94], 42 mer ATCCTGAGAAGTGTTTTTATACATATGTACC 20170216_cc6hb_v3_queen, cell not used, na, na, 202 CCGGTTGATAA na, red, start 21[347], end 7[94], 42 mer TCTTTGATTAGTAATAACATCACCATTAGAT 20170216_cc6hb_v3_queen, cell not used, na, na, 203 ACATTTCGCAA na, red, start 21[431], end 9[94], 42 mer GCCAGCCATTGCAACAGGAAAATAAAAACC 20170216_cc6hb_v3_queen, cell not used, na, na, 204 AAAATAGCGAGA na, red, start 21[515], end 11[94], 42 mer AGATTCACCAGTCACACGACCCTCCATGTTA 20170216_cc6hb_v3_queen, cell not used, na, na, 205 CTTAGCCGGAA na, red, start 21[599], end 13[94], 42 mer ACGTGGCACAGACAATATTTTAAGGAACAA 20170216_cc6hb_v3_queen, cell not used, na, na, 206 CTAAAGGAATTG na, red, start 21[683], end 15[94], 42 mer ATCTGAACTCGCTACGGCGGGGGGAGCCCC 20170407_cc6hb_v3-1_queen, c0, h21, p0, 7 bp 207 CGATTTAGAGCTCGGTCCA plug, cyan, start 0[94], end 21[94], 49 mer CATTGCTGATACCGTTTAGCTAACAAACATC 20170407_cc6hb_v3-1_queen, c0, h20, p1, 7 bp 208 AAGAAAACAAAATGGTGG plug, cyan, start 1[95], end 20[95], 49 mer GATACTTGCCCTCTCTGTACATAATTAATTT 20170407_cc6hb_v3-1_queen, c0, h19, p2, 7 bp 209 TCCCTTAGAATAATAGCC plug, cyan, start 0[178], end 19[94], 49 mer GATTGGGCGTTATCAATGTTGTTTTGTCACA 20170407_cc6hb_v3-1_queen, c0, h18, p3, 7 bp 210 ATCAATAGAAAAGAGTCC plug, cyan, start 1[179], end 18[95], 49 mer TCTAATGAAGACAAATCCCCACGTCACCGA 20170407_cc6hb_v3-1_queen, c0, h17, p4, 7 bp 211 CTTGAGCCATTTGAAAAAC plug, cyan, start 0[262], end 17[94], 49 mer AAACATCGGGTTGAGTATTATGTGGCGAGA 20170407_cc6hb_v3-1_queen, c1, h21, p4, 7 bp 212 AAGGAAGGGAAGGAAAAAC plug, cyan, start 1[53], end 21[136], 49 mer CGCTGGCATTCGCATCAAAGGCGAATTATT 20170407_cc6hb_v3-1_queen, c1, h20, p3, 7 bp 213 CATTTCAATTACAGAGTCC plug, cyan, start 2[136], end 20[137], 49 mer AGTTTATAAATGAGTATCAATTTAGATTAAG 20170407_cc6hb_v3-1_queen, c1, h19, p2, 7 bp 214 ACGCTGAGAAGAATAGCC plug, cyan, start 1[137], end 19[136], 49 mer TATCGACATCATTACGCATCGCAACATATA 20170407_cc6hb_v3-1_queen, c1, h18, p1, 7 bp 215 AAAGAAACGCAAATGGTGG plug, cyan, start 2[220], end 18[137], 49 mer CCATGCAGACATCACGAAGGTCACCAGTAG 20170407_cc6hb_v3-1_queen, c1, h17, p0, 7 bp 216 CACCATTACCATCGGTCCA plug, cyan, start 1[221], end 17[136], 49 mer AAGATAACGCTTGTGAAAATGAGGGCGCTG 20170407_cc6hb_v3-1_queen, c2, h21, p0, 7 bp 217 GCAAGTGTAGCGCGGTCCA plug, cyan, start 2[94], end 21[178], 49 mer GCTAACAGTAGGGAAACTGCGGCCTGATTG 20170407_cc6hb_v3-1_queen, c2, h20, p1, 7 bp 218 CTTTGAATACCAATGGTGG plug, cyan, start 3[95], end 20[179], 49 mer ATGGGTTCAGGATGCAGGTGAAATCATAGG 20170407_cc6hb_v3-1_queen, c2, h19, p2, 7 bp 219 TCTGAGAGACTAAATAGCC plug, cyan, start 2[178], end 19[178], 49 mer CTCGGATGGGAGTAAGCGTATGCAGTATGT 20170407_cc6hb_v3-1_queen, c2, h18, p3, 7 bp 220 TAGCAAACGTAGAGAGTCC plug, cyan, start 3[179], end 18[179], 49 mer AGAGTTTCTGCGGCAGTTAATCAATGAAAC 20170407_cc6hb_v3-1_queen, c2, h17, p4, 7 bp 221 CATCGATAGCAGGAAAAAC plug, cyan, start 2[262], end 17[178], 49 mer GCAATACATCAAACGCCGCGAACACCCGCC 20170407_cc6hb_v3-1_queen, c3, h21, p4, 7 bp 222 GCGCTTAATGCGGAAAAAC plug, cyan, start 3[53], end 21[220], 49 mer TCAGGCACTGCGTGAAGCGGCAGTAACAGT 20170407_cc6hb_v3-1_queen, c3, h20, p3, 7 bp 223 ACCTTTTACATCAGAGTCC plug, cyan, start 4[136], end 20[221], 49 mer ATCAAAACTCAACGAGCAGCGGTTGGGTTA 20170407_cc6hb_v3-1_queen, c3, h19, p2, 7 bp 224 TATAACTATATGAATAGCC plug, cyan, start 3[137], end 19[220], 49 mer AGGGTTGTCGGACTTGTGCAAGGAATACCC 20170407_cc6hb_v3-1_queen, c3, h18, p1, 7 bp 225 AAAAGAACTGGCATGGTGG plug, cyan, start 4[220], end 18[221], 49 mer AGTCCGTGAAGACGGAAACCAAATCAAGTT 20170407_cc6hb_v3-1_queen, c3, h17, p0, 7 bp 226 TGCCTTTAGCGTCGGTCCA plug, cyan, start 3[221], end 17[220], 49 mer CTGGGGATTTGACGCAGACCTGGTTGCTTTG 20170407_cc6hb_v3-1_queen, c4, h21, p0, 7 bp 227 ACGAGCACGTACGGTCCA plug, cyan, start 4[94], end 21[262], 49 mer TTTTCCCAGTCACGACGTTGTGAAATTGCGT 20170407_cc6hb_v3-1_queen, c4, h20, p1, 7 bp 228 AGATTTTCAGGATGGTGG plug, cyan, start 5[95], end 20[263], 49 mer TTATCAGTAAACAGAGAGGTTTCGCAAGAC 20170407_cc6hb_v3-1_queen, c4, h19, p2, 7 bp 229 AAAGAACGCGAGAATAGCC plug, cyan, start 4[178], end 19[262], 49 mer TCAGGGATTAATGAAAGATGGAACAAAGTT 20170407_cc6hb_v3-1_queen, c4, h18, p3, 7 bp 230 ACCAGAAGGAAAAGAGTCC plug, cyan, start 5[179], end 18[263], 49 mer AGTGTGGCGATCCGATAGATGCGGCATTTT 20170407_cc6hb_v3-1_queen, c4, h17, p4, 7 bp 231 CGGTCATAGCCCGAAAAAC plug, cyan, start 4[262], end 17[262], 49 mer GGGGGATGTGCTGCAAGGCGAATCAGAGCG 20170407_cc6hb_v3-1_queen, c5, h21, p4, 7 bp 232 GGAGCTAAACAGGAAAAAC plug, cyan, start 5[53], end 21[304], 49 mer AGCCAGCTTTCCGGCACCGCTACCTACCATA 20170407_cc6hb_v3-1_queen, c5, h20, p3, 7 bp 233 TCAAAATTATTAGAGTCC plug, cyan, start 6[136], end 20[305], 49 mer CTTTATTATTCGCATTCACCCTAGTTAATTTC 20170407_cc6hb_v3-1_queen, c5, h19, p2, 7 bp 234 ATCTTCTGACAATAGCC plug, cyan, start 5[137], end 19[304], 49 mer TTGGTGTAGATGGGCGCATCGATCTTACCG 20170407_cc6hb_v3-1_queen, c5, h18, p1, 7 bp 235 AAGCCCTTTTTAATGGTGG plug, cyan, start 6[220], end 18[305], 49 mer CAGAAATAGAAGAATTACAGCTTTCATAAT 20170407_cc6hb_v3-1_queen, c5, h17, p0, 7 bp 236 CAAAATCACCGGCGGTCCA plug, cyan, start 5[221], end 17[304], 49 mer AAGCGCCATTCGCCATTCAGGAGACAGGAA 20170407_cc6hb_v3-1_queen, c6, h21, p0, 7 bp 237 CGGTACGCCAGACGGTCCA plug, cyan, start 6[94], end 21[346], 49 mer TCAGAAAAGCCCCAAAAACAGCTGATTGTT 20170407_cc6hb_v3-1_queen, c6, h20, p1, 7 bp 238 TGGATTATACTTATGGTGG plug, cyan, start 7[95], end 20[347], 49 mer GAGGGGACGACGACAGTATCGACCGACCGT 20170407_cc6hb_v3-1_queen, c6, h19, p2, 7 bp 239 GTGATAAATAAGAATAGCC plug, cyan, start 6[178], end 19[346], 49 mer TTTTTGTTAAATCAGCTCATTAGCCCAATAA 20170407_cc6hb_v3-1_queen, c6, h18, p3, 7 bp 240 TAAGAGCAAGAAGAGTCC plug, cyan, start 7[179], end 18[347], 49 mer GTGGGAACAAACGGCGGATTGCGCCTCCCT 20170407_cc6hb_v3-1_queen, c6, h17, p4, 7 bp 241 CAGAGCCGCCACGAAAAAC plug, cyan, start 6[262], end 17[346], 49 mer CGAACGAGTAGATTTAGTTTGACTTGCCTGA 20170407_cc6hb_v3-1_queen, c9, h21, p4, 7 bp 242 GTAGAAGAACTGAAAAAC plug, cyan, start 9[53], end 21[472], 49 mer CATTTTTGCGGATGGCTTAGACCGAACGTTA 20170407_cc6hb_v3-1_queen, c9, h20, p3, 7 bp 243 TTAATTTTAAAAGAGTCC plug, cyan, start 10[136], end 20[473], 49 mer AGCTGAAAAGGTGGCATCAATTAGGGCTTA 20170407_cc6hb_v3-1_queen, c9, h19, p2, 7 bp 244 ATTGAGAATCGCAATAGCC plug, cyan, start 9[137], end 19[472], 49 mer AGCTTCAAAGCGAACCAGACCTTTACAGAG 20170407_cc6hb_v3-1_queen, c9, h18, p1, 7 bp 245 AGAATAACATAAATGGTGG plug, cyan, start 10[220], end 18[473], 49 mer ATTAAGCAATAAAGCCTCAGAGGCCTTGAT 20170407_cc6hb_v3-1_queen, c9, h17, p0, 7 bp 246 ATTCACAAACAACGGTCCA plug, cyan, start 9[221], end 17[472], 49 mer CTGTAGCTCAACATGTTTTAAAATATCCAGA 20170407_cc6hb_v3-1_queen, c10, h21, p0, 7 bp 247 ACAATATTACCCGGTCCA plug, cyan, start 10[94], end 21[514], 49 mer GGCTTTTGCAAAAGAAGTTTTAGACTTTACA 20170407_cc6hb_v3-1_queen, c10, h20, p1, 7 bp 248 AACAATTCGACATGGTGG plug, cyan, start 11[95], end 20[515], 49 mer AGGATTAGAGAGTACCTTTAAGTAATTTAG 20170407_cc6hb_v3-1_queen, c10, h19, p2, 7 bp 249 GCAGAGGCATTTAATAGCC plug, cyan, start 10[178], end 19[514], 49 mer AATATTCATTGAATCCCCCTCGAAACGATTT 20170407_cc6hb_v3-1_queen, c10, h18, p3, 7 bp 250 TTTGTTTAACGAGAGTCC plug, cyan, start 11[179], end 18[515], 49 mer AAGAGGAAGCCCGAAAGACTTAATGGAAA 20170407_cc6hb_v3-1_queen, c10, h17, p4, 7 bp 251 GCGCAGTCTCTGAGAAAAAC plug, cyan, start 10[262], end 17[514], 49 mer ACCCTCGTTTACCAGACGACGAACGCTCAT 20170407_cc6hb_v3-1_queen, c11, h21, p4, 7 bp 252 GGAAATACCTACGAAAAAC plug, cyan, start 11[53], end 21[556], 49 mer TAACGGAACAACATTATTACAAGAGCCGTC 20170407_cc6hb_v3-1_queen, c11, h20, p3, 7 bp 253 AATAGATAATACAGAGTCC plug, cyan, start 12[136], end 20[557], 49 mer ATGTTTAGACTGGATAGCGTCATAAAGTAC 20170407_cc6hb_v3-1_queen, c11, h19, p2, 7 bp 254 CGACAAAAGGTAAATAGCC plug, cyan, start 11[137], end 19[556], 49 mer TGAATTACCTTATGCGATTTTTTACAAAATA 20170407_cc6hb_v3-1_queen, c11, h18, p1, 7 bp 255 AACAGCCATATATGGTGG plug, cyan, start 12[220], end 18[557], 49 mer AAACGAGAATGACCATAAATCCATACATGG 20170407_cc6hb_v3-1_queen, c11, h17, p0, 7 bp 256 CTTTTGATGATACGGTCCA plug, cyan, start 11[221], end 17[556], 49 mer AGATTTAGGAATACCACATTCAAATGGATT 20170407_cc6hb_v3-1_queen, c12, h21, p0, 7 bp 257 ATTTACATTGGCCGGTCCA plug, cyan, start 12[94], end 21[598], 49 mer CGAGGCGCAGACGGTCAATCAGTTATCTAA 20170407_cc6hb_v3-1_queen, c12, h20, p1, 7 bp 258 AATATCTTTAGGATGGTGG plug, cyan, start 13[95], end 20[599], 49 mer GTCAGGACGTTGGGAAGAAAAGACAATAA 20170407_cc6hb_v3-1_queen, c12, h19, p2, 7 bp 259 ACAACATGTTCAGAATAGCC plug, cyan, start 12[178], end 19[598], 49 mer AGGCTGGCTGACCTTCATCAATACCAACGC 20170407_cc6hb_v3-1_queen, c12, h18, p3, 7 bp 260 TAACGAGCGTCTAGAGTCC plug, cyan, start 13[179], end 18[599], 49 mer TAAATTGGGCTTGAGATGGTTTTTTAACGGG 20170407_cc6hb_v3-1_queen, c12, h17, p4, 7 bp 261 GTCAGTGCCTTGAAAAAC plug, cyan, start 12[262], end 17[598], 49 mer TGTGTCGAAATCCGCGACCTGAGTAATAAA 20170407_cc6hb_v3-1_queen, c13, h21, p4, 7 bp 262 AGGGACATTCTGGAAAAAC plug, cyan, start 13[53], end 21[640], 49 mer TACGAAGGCACCAACCTAAAACTGGTCAGT 20170407_cc6hb_v3-1_queen, c13, h20, p3, 7 bp 263 TGGCAAATCAACAGAGTCC plug, cyan, start 14[136], end 20[641], 49 mer CTTTGAAAGAGGACAGATGAATATCAACAA 20170407_cc6hb_v3-1_queen, c13, h19, p2, 7 bp 264 TAGATAAGTCCTAATAGCC plug, cyan, start 13[137], end 19[640], 49 mer GTAGCAACGGCTACAGAGGCTTAGTTGCTA 20170407_cc6hb_v3-1_queen, c13, h18, p1, 7 bp 265 TTTTGCACCCAGATGGTGG plug, cyan, start 14[220], end 18[641], 49 mer GATATTCATTACCCAAATCAACAGTTAATGC 20170407_cc6hb_v3-1_queen, c13, h17, p0, 7 bp 266 CCCCTGCCTATCGGTCCA plug, cyan, start 13[221], end 17[640], 49 mer CTAAAACACTCATCTTTGACCCTGACCTGAA 20170407_cc6hb_v3-1_queen, c14, h21, p0, 7 bp 267 AGCGTAAGAATCGGTCCA plug, cyan, start 14[94], end 21[682], 49 mer CGAATAATAATTTTTTCACGTATCACCTTGC 20170407_cc6hb_v3-1_queen, c14, h20, p1, 7 bp 268 TGAACCTCAAAATGGTGG plug, cyan, start 15[95], end 20[683], 49 mer ATGAGGAAGTTTCCATTAAACATCCTAATTT 20170407_cc6hb_v3-1_queen, c14, h19, p2, 7 bp 269 ACGAGCATGTAAATAGCC plug, cyan, start 14[178], end 19[682], 49 mer TTCGAGGTGAATTTCTTAAACACCTCCCGAC 20170407_cc6hb_v3-1_queen, c14, h18, p3, 7 bp 270 TTGCGGGAGGTAGAGTCC plug, cyan, start 15[179], end 18[683], 49 mer GGATCGTCACCCTCAGCAGCGACATGAAAG 20170407_cc6hb_v3-1_queen, c14, h17, p4, 7 bp 271 TATTAAGAGGCTGAAAAAC plug, cyan, start 14[262], end 17[682], 49 mer TTTCAGCGGAGTGAGAATAGATGAATGGCT 20170407_cc6hb_v3-1_queen, c15, h21, p4, 7 bp 272 ATTAGTCTTTAAGAAAAAC plug, cyan, start 15[53], end 21[724], 49 mer CTACAACGCCTGTAGCATTCCAGTGCCACG 20170407_cc6hb_v3-1_queen, c15, h20, p3, 7 bp 273 CTGAGAGCCAGCAGAGTCC plug, cyan, start 16[136], end 20[725], 49 mer CTCCAAAAGGAGCCTTTAATTGTCTTTCCTT 20170407_cc6hb_v3-1_queen, c15, h19, p2, 7 bp 274 ATCATTCCAAGAATAGCC plug, cyan, start 15[137], end 19[724], 49 mer CAGAGCCACCACCCTCATTTTAAGGCTTATC 20170407_cc6hb_v3-1_queen, c15, h18, p1, 7 bp 275 CGGTATTCTAAATGGTGG plug, cyan, start 16[220], end 18[725], 49 mer CCGACAATGACAACAACCATCTAGGATTAG 20170407_cc6hb_v3-1_queen, c15, h17, p0, 7 bp 276 CGGGGTTTTGCTCGGTCCA plug, cyan, start 15[221], end 17[724], 49 mer ATCTGAACTCGCTACGGCGGGGGGAGCCCC 20170407_cc6hb_v3-1_queen, c0, h21, p0, 10 bp 277 CGATTTAGAGCTCGGTCCACGC plug, cyan, start 0[94], end 21[94], 52 mer CATTGCTGATACCGTTTAGCTAACAAACATC 20170407_cc6hb_v3-1_queen, c0, h20, p1, 10 bp 278 AAGAAAACAAAATGGTGGTTC plug, cyan, start 1[95], end 20[95], 52 mer GATACTTGCCCTCTCTGTACATAATTAATTT 20170407_cc6hb_v3-1_queen, c0, h19, p2, 10 bp 279 TCCCTTAGAATAATAGCCCGA plug, cyan, start 0[178], end 19[94], 52 mer GATTGGGCGTTATCAATGTTGTTTTGTCACA 20170407_cc6hb_v3-1_queen, c0, h18, p3, 10 bp 280 ATCAATAGAAAAGAGTCCACT plug, cyan, start 1[179], end 18[95], 52 mer TCTAATGAAGACAAATCCCCACGTCACCGA 20170407_cc6hb_v3-1_queen, c0, h17, p4, 10 bp 281 CTTGAGCCATTTGAAAAACCGT plug, cyan, start 0[262], end 17[94], 52 mer AAACATCGGGTTGAGTATTATGTGGCGAGA 20170407_cc6hb_v3-1_queen, c1, h21, p4, 10 bp 282 AAGGAAGGGAAGGAAAAACCGT plug, cyan, start 1[53], end 21[136], 52 mer CGCTGGCATTCGCATCAAAGGCGAATTATT 20170407_cc6hb_v3-1_queen, c1, h20, p3, 10 bp 283 CATTTCAATTACAGAGTCCACT plug, cyan, start 2[136], end 20[137], 52 mer AGTTTATAAATGAGTATCAATTTAGATTAAG 20170407_cc6hb_v3-1_queen, c1, h19, p2, 10 bp 284 ACGCTGAGAAGAATAGCCCGA plug, cyan, start 1[137], end 19[136], 52 mer TATCGACATCATTACGCATCGCAACATATA 20170407_cc6hb_v3-1_queen, c1, h18, p1, 10 bp 285 AAAGAAACGCAAATGGTGGTTC plug, cyan, start 2[220], end 18[137], 52 mer CCATGCAGACATCACGAAGGTCACCAGTAG 20170407_cc6hb_v3-1_queen, c1, h17, p0, 10 bp 286 CACCATTACCATCGGTCCACGC plug, cyan, start 1[221], end 17[136], 52 mer AAGATAACGCTTGTGAAAATGAGGGCGCTG 20170407_cc6hb_v3-1_queen, c2, h21, p0, 10 bp 287 GCAAGTGTAGCGCGGTCCACGC plug, cyan, start 2[94], end 21[178], 52 mer GCTAACAGTAGGGAAACTGCGGCCTGATTG 20170407_cc6hb_v3-1_queen, c2, h20, p1, 10 bp 288 CTTTGAATACCAATGGTGGTTC plug, cyan, start 3[95], end 20[179], 52 mer ATGGGTTCAGGATGCAGGTGAAATCATAGG 20170407_cc6hb_v3-1_queen, c2, h19, p2, 10 bp 289 TCTGAGAGACTAAATAGCCCGA plug, cyan, start 2[178], end 19[178], 52 mer CTCGGATGGGAGTAAGCGTATGCAGTATGT 20170407_cc6hb_v3-1_queen, c2, h18, p3, 10 bp 290 TAGCAAACGTAGAGAGTCCACT plug, cyan, start 3[179], end 18[179], 52 mer AGAGTTTCTGCGGCAGTTAATCAATGAAAC 20170407_cc6hb_v3-1_queen, c2, h17, p4, 10 bp 291 CATCGATAGCAGGAAAAACCGT plug, cyan, start 2[262], end 17[178], 52 mer GCAATACATCAAACGCCGCGAACACCCGCC 20170407_cc6hb_v3-1_queen, c3, h21, p4, 10 bp 292 GCGCTTAATGCGGAAAAACCGT plug, cyan, start 3[53], end 21[220], 52 mer TCAGGCACTGCGTGAAGCGGCAGTAACAGT 20170407_cc6hb_v3-1_queen, c3, h20, p3, 10 bp 293 ACCTTTTACATCAGAGTCCACT plug, cyan, start 4[136], end 20[221], 52 mer ATCAAAACTCAACGAGCAGCGGTTGGGTTA 20170407_cc6hb_v3-1_queen, c3, h19, p2, 10 bp 294 TATAACTATATGAATAGCCCGA plug, cyan, start 3[137], end 19[220], 52 mer AGGGTTGTCGGACTTGTGCAAGGAATACCC 20170407_cc6hb_v3-1_queen, c3, h18, p1, 10 bp 295 AAAAGAACTGGCATGGTGGTTC plug, cyan, start 4[220], end 18[221], 52 mer AGTCCGTGAAGACGGAAACCAAATCAAGTT 20170407_cc6hb_v3-1_queen, c3, h17, p0, 10 bp 296 TGCCTTTAGCGTCGGTCCACGC plug, cyan, start 3[221], end 17[220], 52 mer CTGGGGATTTGACGCAGACCTGGTTGCTTTG 20170407_cc6hb_v3-1_queen, c4, h21, p0, 10 bp 297 ACGAGCACGTACGGTCCACGC plug, cyan, start 4[94], end 21[262], 52 mer TTTTCCCAGTCACGACGTTGTGAAATTGCGT 20170407_cc6hb_v3-1_queen, c4, h20, p1, 10 bp 298 AGATTTTCAGGATGGTGGTTC plug, cyan, start 5[95], end 20[263], 52 mer TTATCAGTAAACAGAGAGGTTTCGCAAGAC 20170407_cc6hb_v3-1_queen, c4, h19, p2, 10 bp 299 AAAGAACGCGAGAATAGCCCGA plug, cyan, start 4[178], end 19[262], 52 mer TCAGGGATTAATGAAAGATGGAACAAAGTT 20170407_cc6hb_v3-1_queen, c4, h18, p3, 10 bp 300 ACCAGAAGGAAAAGAGTCCACT plug, cyan, start 5[179], end 18[263], 52 mer AGTGTGGCGATCCGATAGATGCGGCATTTT 20170407_cc6hb_v3-1_queen, c4, h17, p4, 10 bp 301 CGGTCATAGCCCGAAAAACCGT plug, cyan, start 4[262], end 17[262], 52 mer GGGGGATGTGCTGCAAGGCGAATCAGAGCG 20170407_cc6hb_v3-1_queen, c5, h21, p4, 10 bp 302 GGAGCTAAACAGGAAAAACCGT plug, cyan, start 5[53], end 21[304], 52 mer AGCCAGCTTTCCGGCACCGCTACCTACCATA 20170407_cc6hb_v3-1_queen, c5, h20, p3, 10 bp 303 TCAAAATTATTAGAGTCCACT plug, cyan, start 6[136], end 20[305], 52 mer CTTTATTATTCGCATTCACCCTAGTTAATTTC 20170407_cc6hb_v3-1_queen, c5, h19, p2, 10 bp 304 ATCTTCTGACAATAGCCCGA plug, cyan, start 5[137], end 19[304], 52 mer TTGGTGTAGATGGGCGCATCGATCTTACCG 20170407_cc6hb_v3-1_queen, c5, h18, p1, 10 bp 305 AAGCCCTTTTTAATGGTGGTTC plug, cyan, start 6[220], end 18[305], 52 mer CAGAAATAGAAGAATTACAGCTTTCATAAT 20170407_cc6hb_v3-1_queen, c5, h17, p0, 10 bp 306 CAAAATCACCGGCGGTCCACGC plug, cyan, start 5[221], end 17[304], 52 mer AAGCGCCATTCGCCATTCAGGAGACAGGAA 20170407_cc6hb_v3-1_queen, c6, h21, p0, 10 bp 307 CGGTACGCCAGACGGTCCACGC plug, cyan, start 6[94], end 21[346], 52 mer TCAGAAAAGCCCCAAAAACAGCTGATTGTT 20170407_cc6hb_v3-1_queen, c6, h20, p1, 10 bp 308 TGGATTATACTTATGGTGGTTC plug, cyan, start 7[95], end 20[347], 52 mer GAGGGGACGACGACAGTATCGACCGACCGT 20170407_cc6hb_v3-1_queen, c6, h19, p2, 10 bp 309 GTGATAAATAAGAATAGCCCGA plug, cyan, start 6[178], end 19[346], 52 mer TTTTTGTTAAATCAGCTCATTAGCCCAATAA 20170407_cc6hb_v3-1_queen, c6, h18, p3, 10 bp 310 TAAGAGCAAGAAGAGTCCACT plug, cyan, start 7[179], end 18[347], 52 mer GTGGGAACAAACGGCGGATTGCGCCTCCCT 20170407_cc6hb_v3-1_queen, c6, h17, p4, 10 bp 311 CAGAGCCGCCACGAAAAACCGT plug, cyan, start 6[262], end 17[346], 52 mer CGAACGAGTAGATTTAGTTTGACTTGCCTGA 20170407_cc6hb_v3-1_queen, c9, h21, p4, 10 bp 312 GTAGAAGAACTGAAAAACCGT plug, cyan, start 9[53], end 21[472], 52 mer CATTTTTGCGGATGGCTTAGACCGAACGTTA 20170407_cc6hb_v3-1_queen, c9, h20, p3, 10 bp 313 TTAATTTTAAAAGAGTCCACT plug, cyan, start 10[136], end 20[473], 52 mer AGCTGAAAAGGTGGCATCAATTAGGGCTTA 20170407_cc6hb_v3-1_queen, c9, h19, p2, 10 bp 314 ATTGAGAATCGCAATAGCCCGA plug, cyan, start 9[137], end 19[472], 52 mer AGCTTCAAAGCGAACCAGACCTTTACAGAG 20170407_cc6hb_v3-1_queen, c9, h18, p1, 10 bp 315 AGAATAACATAAATGGTGGTTC plug, cyan, start 10[220], end 18[473], 52 mer ATTAAGCAATAAAGCCTCAGAGGCCTTGAT 20170407_cc6hb_v3-1_queen, c9, h17, p0, 10 bp 316 ATTCACAAACAACGGTCCACGC plug, cyan, start 9[221], end 17[472], 52 mer CTGTAGCTCAACATGTTTTAAAATATCCAGA 20170407_cc6hb_v3-1_queen, c10, h21, p0, 10 bp 317 ACAATATTACCCGGTCCACGC plug, cyan, start 10[94], end 21[514], 52 mer GGCTTTTGCAAAAGAAGTTTTAGACTTTACA 20170407_cc6hb_v3-1_queen, c10, h20, p1, 10 bp 318 AACAATTCGACATGGTGGTTC plug, cyan, start 11[95], end 20[515], 52 mer AGGATTAGAGAGTACCTTTAAGTAATTTAG 20170407_cc6hb_v3-1_queen, c10, h19, p2, 10 bp 319 GCAGAGGCATTTAATAGCCCGA plug, cyan, start 10[178], end 19[514], 52 mer AATATTCATTGAATCCCCCTCGAAACGATTT 20170407_cc6hb_v3-1_queen, c10, h18, p3, 10 bp 320 TTTGTTTAACGAGAGTCCACT plug, cyan, start 11[179], end 18[515], 52 mer AAGAGGAAGCCCGAAAGACTTAATGGAAA 20170407_cc6hb_v3-1_queen, c10, h17, p4, 10 bp 321 GCGCAGTCTCTGAGAAAAACCGT plug, cyan, start 10[262], end 17[514], 52 mer ACCCTCGTTTACCAGACGACGAACGCTCAT 20170407_cc6hb_v3-1_queen, c11, h21, p4, 10 bp 322 GGAAATACCTACGAAAAACCGT plug, cyan, start 11[53], end 21[556], 52 mer TAACGGAACAACATTATTACAAGAGCCGTC 20170407_cc6hb_v3-1_queen, c11, h20, p3, 10 bp 323 AATAGATAATACAGAGTCCACT plug, cyan, start 12[136], end 20[557], 52 mer ATGTTTAGACTGGATAGCGTCATAAAGTAC 20170407_cc6hb_v3-1_queen, c11, h19, p2, 10 bp 324 CGACAAAAGGTAAATAGCCCGA plug, cyan, start 11[137], end 19[556], 52 mer TGAATTACCTTATGCGATTTTTTACAAAATA 20170407_cc6hb_v3-1_queen, c11, h18, p1, 10 bp 325 AACAGCCATATATGGTGGTTC plug, cyan, start 12[220], end 18[557], 52 mer AAACGAGAATGACCATAAATCCATACATGG 20170407_cc6hb_v3-1_queen, c11, h17, p0, 10 bp 326 CTTTTGATGATACGGTCCACGC plug, cyan, start 11[221], end 17[556], 52 mer AGATTTAGGAATACCACATTCAAATGGATT 20170407_cc6hb_v3-1_queen, c12, h21, p0, 10 bp 327 ATTTACATTGGCCGGTCCACGC plug, cyan, start 12[94], end 21[598], 52 mer CGAGGCGCAGACGGTCAATCAGTTATCTAA 20170407_cc6hb_v3-1_queen, c12, h20, p1, 10 bp 328 AATATCTTTAGGATGGTGGTTC plug, cyan, start 13[95], end 20[599], 52 mer GTCAGGACGTTGGGAAGAAAAGACAATAA 20170407_cc6hb_v3-1_queen, c12, h19, p2, 10 bp 329 ACAACATGTTCAGAATAGCCCGA plug, cyan, start 12[178], end 19[598], 52 mer AGGCTGGCTGACCTTCATCAATACCAACGC 20170407_cc6hb_v3-1_queen, c12, h18, p3, 10 bp 330 TAACGAGCGTCTAGAGTCCACT plug, cyan, start 13[179], end 18[599], 52 mer TAAATTGGGCTTGAGATGGTTTTTTAACGGG 20170407_cc6hb_v3-1_queen, c12, h17, p4, 10 bp 331 GTCAGTGCCTTGAAAAACCGT plug, cyan, start 12[262], end 17[598], 52 mer TGTGTCGAAATCCGCGACCTGAGTAATAAA 20170407_cc6hb_v3-1_queen, c13, h21, p4, 10 bp 332 AGGGACATTCTGGAAAAACCGT plug, cyan, start 13[53], end 21[640], 52 mer TACGAAGGCACCAACCTAAAACTGGTCAGT 20170407_cc6hb_v3-1_queen, c13, h20, p3, 10 bp 333 TGGCAAATCAACAGAGTCCACT plug, cyan, start 14[136], end 20[641], 52 mer CTTTGAAAGAGGACAGATGAATATCAACAA 20170407_cc6hb_v3-1_queen, c13, h19, p2, 10 bp 334 TAGATAAGTCCTAATAGCCCGA plug, cyan, start 13[137], end 19[640], 52 mer GTAGCAACGGCTACAGAGGCTTAGTTGCTA 20170407_cc6hb_v3-1_queen, c13, h18, p1, 10 bp 335 TTTTGCACCCAGATGGTGGTTC plug, cyan, start 14[220], end 18[641], 52 mer GATATTCATTACCCAAATCAACAGTTAATGC 20170407_cc6hb_v3-1_queen, c13, h17, p0, 10 bp 336 CCCCTGCCTATCGGTCCACGC plug, cyan, start 13[221], end 17[640], 52 mer CTAAAACACTCATCTTTGACCCTGACCTGAA 20170407_cc6hb_v3-1_queen, c14, h21, p0, 10 bp 337 AGCGTAAGAATCGGTCCACGC plug, cyan, start 14[94], end 21[682], 52 mer CGAATAATAATTTTTTCACGTATCACCTTGC 20170407_cc6hb_v3-1_queen, c14, h20, p1, 10 bp 338 TGAACCTCAAAATGGTGGTTC plug, cyan, start 15[95], end 20[683], 52 mer ATGAGGAAGTTTCCATTAAACATCCTAATTT 20170407_cc6hb_v3-1_queen, c14, h19, p2, 10 bp 339 ACGAGCATGTAAATAGCCCGA plug, cyan, start 14[178], end 19[682], 52 mer TTCGAGGTGAATTTCTTAAACACCTCCCGAC 20170407_cc6hb_v3-1_queen, c14, h18, p3, 10 bp 340 TTGCGGGAGGTAGAGTCCACT plug, cyan, start 15[179], end 18[683], 52 mer GGATCGTCACCCTCAGCAGCGACATGAAAG 20170407_cc6hb_v3-1_queen, c14, h17, p4, 10 bp 341 TATTAAGAGGCTGAAAAACCGT plug, cyan, start 14[262], end 17[682], 52 mer TTTCAGCGGAGTGAGAATAGATGAATGGCT 20170407_cc6hb_v3-1_queen, c15, h21, p4, 10 bp 342 ATTAGTCTTTAAGAAAAACCGT plug, cyan, start 15[53], end 21[724], 52 mer CTACAACGCCTGTAGCATTCCAGTGCCACG 20170407_cc6hb_v3-1_queen, c15, h20, p3, 10 bp 343 CTGAGAGCCAGCAGAGTCCACT plug, cyan, start 16[136], end 20[725], 52 mer CTCCAAAAGGAGCCTTTAATTGTCTTTCCTT 20170407_cc6hb_v3-1_queen, c15, h19, p2, 10 bp 344 ATCATTCCAAGAATAGCCCGA plug, cyan, start 15[137], end 19[724], 52 mer CAGAGCCACCACCCTCATTTTAAGGCTTATC 20170407_cc6hb_v3-1_queen, c15, h18, p1, 10 bp 345 CGGTATTCTAAATGGTGGTTC plug, cyan, start 16[220], end 18[725], 52 mer CCGACAATGACAACAACCATCTAGGATTAG 20170407_cc6hb_v3-1_queen, c15, h17, p0, 10 bp 346 CGGGGTTTTGCTCGGTCCACGC plug, cyan, start 15[221], end 17[724], 52 mer

TABLE 2 Exemplary 250 nm Six-helix Bundle Sequences SEQ ID Sequence Comment NO: TCATCAACATTAAAAGAACGCGAGAAAATT 20170608 cc6hb v4-base 250 nm 6hb, grey 347 GTTAAATCAGACCGTGCAT standard seq, start 0[41], end 4[21], 49 mer TAATCGTAAAACTAATCTTCTGACCTAAAGC 20170608 cc6hb v4-base 250 nm 6hb, grey 348 TATTTTTGATA standard seq, start 0[83], end 4[70], 42 mer ATATATTTTAAATGGATAAATAAGGCGTAA 20170608 cc6hb v4-base 250 nm 6hb, grey 349 AAACATTATGTC standard seq, start 0[125], end 4[112], 42 mer GCGAGCTGAAAAGGTTACTAGAAAAAGCAC 20170608 cc6hb v4-base 250 nm 6hb, grey 350 GAGTAGATTTCT standard seq, start 0[167], end 4[154], 42 mer GGTCATTTTTGCGGTTCTTACCAGTATATTC 20170608 cc6hb v4-base 250 nm 6hb, grey 351 AAAGCGAACCC standard seq, start 0[209], end 4[196], 42 mer AGAAAACGAGAATGTGAGAATCGCCATATT 20170608 cc6hb v4-base 250 nm 6hb, grey 352 TAGACTGGATAG standard seq, start 0[251], end 4[238], 42 mer ACGCCAAAAGGAATAGAGGCATTTTCGACG 20170608 cc6hb v4-base 250 nm 6hb, grey 353 GAACAACATTAG standard seq, start 0[293], end 4[280], 42 mer TAGTAAATTGGGCTACAAAAGGTAAAGTAT 20170608 cc6hb v4-base 250 nm 6hb, grey 354 TCATTACCCAAG standard seq, start 0[335], end 4[322], 42 mer GAACGAGGCGCAGAACATGTTCAGCTAAAC 20170608 cc6hb v4-base 250 nm 6hb, grey 355 AAAGTACAACCA standard seq, start 0[377], end 4[364], 42 mer TTCATGAGGAAGTTGATAAGTCCTGAACTC 20170608 cc6hb v4-base 250 nm 6hb, grey 356 GTCACCCTCATT standard seq, start 0[419], end 4[406], 42 mer GCTTTCGAGGTGAACGAGCATGTAGAAAAT 20170608 cc6hb v4-base 250 nm 6hb, grey 357 AATAATTTTTTG standard seq, start 0[461], end 4[448], 42 mer TAGCGTAACGATCTTCATTCCAAGAACGCC 20170608 cc6hb v4-base 250 nm 6hb, grey 358 CATGTACCGTAA standard seq, start 0[503], end 4[490], 42 mer AAGTATAGCCCGGAAGAACAAGCAAGCCAC 20170608 cc6hb v4-base 250 nm 6hb, grey 359 TCCTCAAGAGCA standard seq, start 0[545], end 4[532], 42 mer ATACAGGAGTGTACCCGCGCCCAATAGCAA 20170608 cc6hb v4-base 250 nm 6hb, grey 360 TCCTCATTAATC standard seq, start 0[587], end 4[574], 42 mer CACCCTCAGAACCGGGTATTCTAAGAACTA 20170608 cc6hb v4-base 250 nm 6hb, grey 361 TTAGCGTTTGAC standard seq, start 0[629], end 4[616], 42 mer CATTAGCAAGGCCGTGCGGGAGGTTTTGTT 20170608 cc6hb v4-base 250 nm 6hb, grey 362 AAAGGTGAATTT standard seq, start 0[671], end 4[658], 42 mer CAAAGACACCACGGTTGCACCCAGCTACAT 20170608 cc6hb v4-base 250 nm 6hb, grey 363 TAAGACTCCTGG standard seq, start 0[713], end 4[700], 42 mer AGAAACAATGAAATACGAGCGTCTTTCCGA 20170608 cc6hb v4-base 250 nm 6hb, grey 364 ATTAACTGAAAA standard seq, start 0[755], end 4[742], 42 mer GAACAAACGGCGGATTGACAATAATTCG 20170608 cc6hb v4-base 250 nm 6hb, grey 365 standard seq, start 1[7], end 1[34], 28 mer CGTCTGGCCTTCCTGTCCCGGTTGATAATCA 20170608 cc6hb v4-base 250 nm 6hb, grey 366 GAAGAGTCTGG standard seq, start 1[35], end 1[76], 42 mer AGCAAACAAGAGAATCAGGTAAAGATTCAA 20170608 cc6hb v4-base 250 nm 6hb, grey 367 AAGTTTCAACGC standard seq, start 1[77], end 1[118], 42 mer AAGGATAAAAATTTTTAGTAGTAGCATTAA 20170608 cc6hb v4-base 250 nm 6hb, grey 368 CATCAATAACCT standard seq, start 1[119], end 1[160], 42 mer GTTTAGCTATATTTTCCTGAATATAATGCTG 20170608 cc6hb v4-base 250 nm 6hb, grey 369 TATAGAGAGTA standard seq, start 1[161], end 1[202], 42 mer CCTTTAATTGCTCCTTGTCTTTACCCTGACTA 20170608 cc6hb v4-base 250 nm 6hb, grey 370 TTCATTGAAT standard seq, start 1[203], end 1[244], 42 mer CCCCCTCAAATGCTTTAACACTATCATAACC 20170608 cc6hb v4-base 250 nm 6hb, grey 371 CTTAGGAATAC standard seq, start 1[245], end 1[286], 42 mer CACATTCAACTAATGCCTTTAATCATTGTGA 20170608 cc6hb v4-base 250 nm 6hb, grey 372 ATTAAGGCTTG standard seq, start 1[287], end 1[328], 42 mer CCCTGACGAGAAACACCGAACTGACCAACT 20170608 cc6hb v4-base 250 nm 6hb, grey 373 TTGCGAAATCCG standard seq, start 1[329], end 1[370], 42 mer CGACCTGCTCCATGTTACGTAATGCCACTAC 20170608 cc6hb v4-base 250 nm 6hb, grey 374 GAAACGGCTAC standard seq, start 1[371], end 1[412], 42 mer AGAGGCTTTGAGGACTCCGATAGTTGCGCC 20170608 cc6hb v4-base 250 nm 6hb, grey 375 GACAAAGGAGCC standard seq, start 1[413], end 1[454], 42 mer TTTAATTGTATCGGTTAGACGTTAGTAAATG 20170608 cc6hb v4-base 250 nm 6hb, grey 376 AAACGCCTGTA standard seq, start 1[455], end 1[496], 42 mer GCATTCCACAGACAGCCAGGAGGTTTAGTA 20170608 cc6hb v4-base 250 nm 6hb, grey 377 CCGCCAGGCGGA standard seq, start 1[497], end 1[538], 42 mer TAAGTGCCGTCGAGAGGGTCAGTGCCTTGA 20170608 cc6hb v4-base 250 nm 6hb, grey 378 GTACCGTTCCAG standard seq, start 1[539], end 1[580], 42 mer TAAGCGTCATACATGGCCTCAGAGCCGCCA 20170608 cc6hb v4-base 250 nm 6hb, grey 379 CCAGAGCCACCA standard seq, start 1[581], end 1[622], 42 mer CCGGAACCGCCTCCCTCATCGATAGCAGCA 20170608 cc6hb v4-base 250 nm 6hb, grey 380 CCGTTAGAGCCA standard seq, start 1[623], end 1[664], 42 mer GCAAAATCACCAGTAGAATCAATAGAAAAT 20170608 cc6hb v4-base 250 nm 6hb, grey 381 TCAACATACATA standard seq, start 1[665], end 1[706], 42 mer AAGGTGGCAACATATAAAGCCCTTTTTAAG 20170608 cc6hb v4-base 250 nm 6hb, grey 382 AAAAGAATTGAGTTAAGCC standard seq, start 1[707], end 1[755], 49 mer ACAGGAAGATTGAATAGGAACGCCATCAAA 20170608 cc6hb v4-base 250 nm 6hb, grey 383 CGTAATGGGATA standard seq, start 2[55], end 2[14], 42 mer AGATGGGCGCATCGTACTCATTTTTTAACCT 20170608 cc6hb v4-base 250 nm 6hb, grey 384 ATAA standard seq, start 3[14], end 3[48], 35 mer GCAAATATTTAAATTGTAAACGTGAGATCT 20170608 cc6hb v4-base 250 nm 6hb, grey 385 ACAAAGGAATCA standard seq, start 3[49], end 3[90], 42 mer CCATCAATATGATATTCAACCGTACCCTGTA 20170608 cc6hb v4-base 250 nm 6hb, grey 386 ATACTTAAGAA standard seq, start 3[91], end 3[132], 42 mer TTAGCAAAATTAAGCAATAAAGCAGTTTGA 20170608 cc6hb v4-base 250 nm 6hb, grey 387 CCATTAGCTAAA standard seq, start 3[133], end 3[174], 42 mer GTACGGTGTCTGGAAGTTTCATTCAGACCGG 20170608 cc6hb v4-base 250 nm 6hb, grey 388 AAGCAACATCA standard seq, start 3[175], end 3[216], 42 mer AAAAGATTAAGAGGAAGCCCGAAAGCGTCC 20170608 cc6hb v4-base 250 nm 6hb, grey 389 AATACTGCAAAA standard seq, start 3[217], end 3[258], 42 mer TAGCGAGAGGCTTTTGCAAAAGAATTACAG 20170608 cc6hb v4-base 250 nm 6hb, grey 390 GTAGAAAGCTCA standard seq, start 3[259], end 3[300], 42 mer TTATACCAGTCAGGACGTTGGGAAATCAAC 20170608 cc6hb v4-base 250 nm 6hb, grey 391 GTAACAAAGACC standard seq, start 3[301], end 3[342], 42 mer AGGCGCATAGGCTGGCTGACCTTGGAGATT 20170608 cc6hb v4-base 250 nm 6hb, grey 392 TGTATCAGCAAA standard seq, start 3[343], end 3[384], 42 mer AGAATACACTAAAACACTCATCTGCAGCGA 20170608 cc6hb v4-base 250 nm 6hb, grey 393 AAGACAGATAAC standard seq, start 3[385], end 3[426], 42 mer CGATATATTCGGTCGCTGAGGCTTCACGTTG 20170608 cc6hb v4-base 250 nm 6hb, grey 394 AAAATCCAACT standard seq, start 3[427], end 3[468], 42 mer TTCAACAGTTTCAGCGGAGTGAGAACACTG 20170608 cc6hb v4-base 250 nm 6hb, grey 395 AGTTTCGGAACC standard seq, start 3[469], end 3[510], 42 mer GCCACCCTCAGAGCCACCACCCTAAGGATT 20170608 cc6hb v4-base 250 nm 6hb, grey 396 AGGATTAGCCCC standard seq, start 3[511], end 3[552], 42 mer CTGCCTATTTCGGAACCTATTATAGCCAGAA 20170608 cc6hb v4-base 250 nm 6hb, grey 397 TGGAAAGCATT standard seq, start 3[553], end 3[594], 42 mer GACAGGAGGTTGAGGCAGGTCAGCCATCTT 20170608 cc6hb v4-base 250 nm 6hb, grey 398 TTCATAATTGCC standard seq, start 3[595], end 3[636], 42 mer TTTAGCGTCAGACTGTAGCGCGTTATCACCG 20170608 cc6hb v4-base 250 nm 6hb, grey 399 TCACCGAAAGG standard seq, start 3[637], end 3[678], 42 mer GCGACATTCAACCGATTGAGGGATATTACG 20170608 cc6hb v4-base 250 nm 6hb, grey 400 CAGTATGTACCA standard seq, start 3[679], end 3[720], 42 mer GAAGGAAACCGAGGAAACGCAATCACCCTG 20170608 cc6hb v4-base 250 nm 6hb, grey 401 AACAAAGTCAGATAATATC standard seq, start 3[721], end 2[756], 49 mer ATATTTTGTTAAAATTCGCATTAAATTTCTTT 20170608 cc6hb v4-base 250 nm 6hb, grey 402 TTCAAATATA standard seq, start 4[69], end 5[55], 42 mer TAGCTGATAAATTAATGCCGGAGAGGGTAT 20170608 cc6hb v4-base 250 nm 6hb, grey 403 TTAATGGTTTGA standard seq, start 4[111], end 5[97], 42 mer CAGAGCATAAAGCTAAATCGGTTGTACCTA 20170608 cc6hb v4-base 250 nm 6hb, grey 404 AATAAGAATAAA standard seq, start 4[153], end 5[139], 42 mer ATATAACAGTTGATTCCCAATTCTGCGACTG 20170608 cc6hb v4-base 250 nm 6hb, grey 405 TTTAGTATCAT standard seq, start 4[195], end 5[181], 42 mer ACTTCAAATATCGCGTTTTAATTCGAGCAAG 20170608 cc6hb v4-base 250 nm 6hb, grey 406 CCAACGCTCAA standard seq, start 4[237], end 5[223], 42 mer TTTTGCCAGAGGGGGTAATAGTAAAATGTTT 20170608 cc6hb v4-base 250 nm 6hb, grey 407 AACAACGCCAA standard seq, start 4[279], end 5[265], 42 mer AAAAATCTACGTTAATAAAACGAACTAAGC 20170608 cc6hb v4-base 250 nm 6hb, grey 408 CAGTAATAAGAG standard seq, start 4[321], end 5[307], 42 mer TCAAGAGTAATCTTGACAAGAACCGGATAA 20170608 cc6hb v4-base 250 nm 6hb, grey 409 TTCTGTCCAGAC standard seq, start 4[363], end 5[349], 42 mer GACCCCCAGCGATTATACCAAGCGCGAATG 20170608 cc6hb v4-base 250 nm 6hb, grey 410 CAGAACGCGCCT standard seq, start 4[405], end 5[391], 42 mer CAGGGAGTTAAAGGCCGCTTTTGCGGGAAA 20170608 cc6hb v4-base 250 nm 6hb, grey 411 GAAAAATAATAT standard seq, start 4[447], end 5[433], 42 mer TAGAAAGGAACAACTAAAGGAATTGCGACC 20170608 cc6hb v4-base 250 nm 6hb, grey 412 AATCAATAATCG standard seq, start 4[489], end 5[475], 42 mer TTTTCAGGGATAGCAAGCCCAATAGGAAGG 20170608 cc6hb v4-base 250 nm 6hb, grey 413 TATTAAACCAAG standard seq, start 4[531], end 5[517], 42 mer TGAAACATGAAAGTATTAAGAGGCTGAGGT 20170608 cc6hb v4-base 250 nm 6hb, grey 414 TTTTATTTTCAT standard seq, start 4[573], end 5[559], 42 mer GATTGGCCTTGATATTCACAAACAAATAAA 20170608 cc6hb v4-base 250 nm 6hb, grey 415 GCAAATCAGATA standard seq, start 4[615], end 5[601], 42 mer TCATCGGCATTTTCGGTCATAGCCCCCTGCG 20170608 cc6hb v4-base 250 nm 6hb, grey 416 AGGCGTTTTAG standard seq, start 4[657], end 5[643], 42 mer GAAGGTAAATATTGACGGAAATTATTCAAA 20170608 cc6hb v4-base 250 nm 6hb, grey 417 GCCTTAAATCAA standard seq, start 4[699], end 5[685], 42 mer TAACGGAATACCCAAAAGAACTGGCATGAA 20170608 cc6hb v4-base 250 nm 6hb, grey 418 TTTTATCCTGAA standard seq, start 4[741], end 5[727], 42 mer CGCATTAGACGGGAAGAGCCT 20170608 cc6hb v4-base 250 nm 6hb, grey 419 standard seq, start 4[769], end 5[762], 21 mer AAGACAATGTGAGCGAGTAACAACCCGT 20170608 cc6hb v4-base 250 nm 6hb, grey 420 standard seq, start 5[21], end 0[7], 28 mer TTTTAGTTAATTTCGCATGTCAATCATATGT 20170608 cc6hb v4-base 250 nm 6hb, yellow 421 ACAGCCAGCTT standard seq, start 5[56], end 0[42], 42 mer AATACCGACCGTGTCAATGCCTGAGTAATG 20170608 cc6hb v4-base 250 nm 6hb, yellow 422 TGTGATGAACGG standard seq, start 5[98], end 0[84], 42 mer CACCGGAATCATAATGGCATCAATTCTACT 20170608 cc6hb v4-base 250 nm 6hb, yellow 423 AATAGAACCCTC standard seq, start 5[140], end 0[126], 42 mer ATGCGTTATACAAAATGGCTTAGAGCTTAAT 20170608 cc6hb v4-base 250 nm 6hb, yellow 424 TGATTTGGGGC standard seq, start 5[182], end 0[168], 42 mer CAGTAGGGCTTAATACCATAAATCAAAAAT 20170608 cc6hb v4-base 250 nm 6hb, yellow 425 CAGTTGATAAGA standard seq, start 5[224], end 0[210], 42 mer CATGTAATTTAGGCTACGAGGCATAGTAAG 20170608 cc6hb v4-base 250 nm 6hb, yellow 426 AGCAAACAGTTC standard seq, start 5[266], end 0[252], 42 mer AATATAAAGTACCGTGAGATGGTTTAATTTC 20170608 cc6hb v4-base 250 nm 6hb, yellow 427 AAAGATACATA standard seq, start 5[308], end 0[294], 42 mer GACGACAATAAACACGGTCAATCATAAGGG 20170608 cc6hb v4-base 250 nm 6hb, yellow 428 AACCAGAACGAG standard seq, start 5[350], end 0[336], 42 mer GTTTATCAACAATATCCATTAAACGGGTAA 20170608 cc6hb v4-base 250 nm 6hb, yellow 429 AATACTTAGCCG standard seq, start 5[392], end 0[378], 42 mer CCCATCCTAATTTATTTCTTAAACAGCTTGA 20170608 cc6hb v4-base 250 nm 6hb, yellow 430 TAAAAGACTTT standard seq, start 5[434], end 0[420], 42 mer GCTGTCTTTCCTTAAAAGTTTTGTCGTCTTTC 20170608 cc6hb v4-base 250 nm 6hb, yellow 431 CTATCAGCTT standard seq, start 5[476], end 0[462], 42 mer TACCGCACTCATCGATAGGTGTATCACCGTA 20170608 cc6hb v4-base 250 nm 6hb, yellow 432 CTCCTCATAGT standard seq, start 5[518], end 0[504], 42 mer CGTAGGAATCATTATGGTAATAAGTTTTAAC 20170608 cc6hb v4-base 250 nm 6hb, yellow 433 GGGGTTGATAT standard seq, start 5[560], end 0[546], 42 mer TAGAAGGCTTATCCCCACCCTCAGAGCCAC 20170608 cc6hb v4-base 250 nm 6hb, yellow 434 CACCTTTTGATG standard seq, start 5[602], end 0[588], 42 mer CGAACCTCCCGACTGAAACGTCACCAATGA 20170608 cc6hb v4-base 250 nm 6hb, yellow 435 AACCAGAGCCGC standard seq, start 5[644], end 0[630], 42 mer GATTAGTTGCTATTAATAAGTTTATTTTGTC 20170608 cc6hb v4-base 250 nm 6hb, yellow 436 ACCACCATTAC standard seq, start 5[686], end 0[672], 42 mer TCTTACCAACGCTAAGCAATAGCTATCTTAC 20170608 cc6hb v4-base 250 nm 6hb, yellow 437 CGAAAGAAACG standard seq, start 5[728], end 0[714], 42 mer CGGAGACAGTCACTATCAGGTCATTGCCTG 20170608 cc6hb v4-base 250 nm 6hb, magenta 438 AAAGCCCCAAAA standard seq, start 2[97], end 2[56], 42 mer ACAGGCAAGGCATTGCGGGAGAAGCCTTTA 20170608 cc6hb v4-base 250 nm 6hb, magenta 439 GGTGAGAAAGGC standard seq, start 2[139], end 2[98], 42 mer TTAAATATGCAAATACATTTCGCAAATGGTC 20170608 cc6hb v4-base 250 nm 6hb, magenta 440 CAATAAATCAT standard seq, start 2[181], end 2[140], 42 mer CAAAGCGGATTGACTCCAACAGGTCAGGAT 20170608 cc6hb v4-base 250 nm 6hb, magenta 441 GCTCAACATGTT standard seq, start 2[223], end 2[182], 42 mer GACGATAAAAACCGGAATCGTCATAAATAT 20170608 cc6hb v4-base 250 nm 6hb, magenta 442 TATAGTCAGAAG standard seq, start 2[265], end 2[224], 42 mer TTTTAAGAACTGGATTCATCAGTTGAGATTC 20170608 cc6hb v4-base 250 nm 6hb, magenta 443 GTTTACCAGAC standard seq, start 2[307], end 2[266], 42 mer TGAACGGTGTACAGCTGCTCATTCAGTGAAT 20170608 cc6hb v4-base 250 nm 6hb, magenta 444 ACCTTATGCGA standard seq, start 2[349], end 2[308], 42 mer AAAACGAAAGAGTCGCCTGATAAATTGTGT 20170608 cc6hb v4-base 250 nm 6hb, magenta 445 AAAGAGGACAGA standard seq, start 2[391], end 2[350], 42 mer CATCGCCCACGCCATCGGAACGAGGGTAGC 20170608 cc6hb v4-base 250 nm 6hb, magenta 446 AGGCACCAACCT standard seq, start 2[433], end 2[392], 42 mer GATTTTGCTAAATCCAAAAAAAAGGCTCCA 20170608 cc6hb v4-base 250 nm 6hb, magenta 447 AATGACAACAAC standard seq, start 2[475], end 2[434], 42 mer CCGCCACCCTCATCACCAGTACAAACTACA 20170608 cc6hb v4-base 250 nm 6hb, magenta 448 TTTTCTGTATGG standard seq, start 2[517], end 2[476], 42 mer TAAACAGTTAATGCGGGGTTTTGCTCAGTAC 20170608 cc6hb v4-base 250 nm 6hb, magenta 449 CACCCTCAGAA standard seq, start 2[559], end 2[518], 42 mer GAGCCGCCGCCAGCGCAGTCTCTGAATTTA 20170608 cc6hb v4-base 250 nm 6hb, magenta 450 ACAGTGCCCGTA standard seq, start 2[601], end 2[560], 42 mer ACAGAATCAAGTTCAAAATCACCGGAACCA 20170608 cc6hb v4-base 250 nm 6hb, magenta 451 GAACCACCACCA standard seq, start 2[643], end 2[602], 42 mer GCGCCAAAGACAACTTGAGCCATTTGGGAA 20170608 cc6hb v4-base 250 nm 6hb, magenta 452 TAATCAGTAGCG standard seq, start 2[685], end 2[644], 42 mer CCGAACAAAGTTTAGCAAACGTAGAAAATT 20170608 cc6hb v4-base 250 nm 6hb, magenta 453 ATGGTTTACCA standard seq, start 2[726], end 2[686], 41 mer AGAGAGATAACCCACAAGTAAGCAGATAG 20170608 cc6hb v4-base 250 nm 6hb, magenta 454 standard seq, start 2[755], end 2[727], 29 mer AGAAGATGAAATTAACTAAAATATATTTGAAA 20170608, cc6hb v4-250 nm 16 component square, 455 AAGTTTTCTCGCGTTCTTTGTCTTGCGATTG default 6hb, miniscaf, node-0 ATTTATCACACGGTCGGTATTTCAAACCATT 20170608, cc6hb v4-250 nm 16 component square, 456 AAATTTAGGTC default 6hb, miniscaf, node-1 CTAGTAATTATGATTCCGGTGTTTATTCTTA 20170608, cc6hb v4-250 nm 16 component square, 457 TTTAACGCCTT default 6hb, miniscaf, node-2 GTAAGAATTTGTATAACGCATATGATACTA 20170608, cc6hb v4-250 nm 16 component square, 458 AACAGGCTTTTT default 6hb, miniscaf, node-3 ATTCTCAATTAAGCCCTACTGTTGAGCGTTG 20170608, cc6hb v4-250 nm 16 component square, 459 GCTTTATACTG default 6hb, miniscaf, node-4 TGCCTCTGCCTAAATTACATGTTGGCGTTGT 20170608, cc6hb v4-250 nm 16 component square, 460 TAAATATGGCG default 6hb, miniscaf, node-5 CTTTTGTCGGTACTTTATATTCTCTTATTACT 20170608, cc6hb v4-250 nm 16 component square, 461 GGCTCGAAAA default 6hb, miniscaf, node-6 AACATGTTGTTTATTGTCGTCGTCTGGACAG 20170608, cc6hb v4-250 nm 16 component square, 462 AATTACTTTAC default 6hb, miniscaf, node-7 ACTTATCTATTGTTGATAAACAGGCGCGTTC 20170608, cc6hb v4-250 nm 16 component square, 463 TGCATTAGCTG default 6hb, miniscaf, node-8 ATGCTCGTAAATTAGGATGGGATATTATTTT 20170608, cc6hb v4-250 nm 16 component square, 464 TCTTGTTCAGG default 6hb, miniscaf, node-9 GGAATGATAAGGAAAGACAGCCGATTATTG 20170608, cc6hb v4-250 nm 16 component square, 465 ATTGGTTTCTAC default 6hb, miniscaf, node-10 TTGTTCTCGATGAGTGCGGTACTTGGTTTAA 20170608, cc6hb v4-250 nm 16 component square, 466 TACCCGTTCTT default 6hb, miniscaf, node-11 GGCGCGGTAATGATTCCTACGATGAAAATA 20170608, cc6hb v4-250 nm 16 component square, 467 AAAACGGCTTGC default 6hb, miniscaf, node-12 GAATACCGGATAAGCCTTCTATATCTGATTT 20170608, cc6hb v4-250 nm 16 component square, 468 GCTTGCTATTG default 6hb, miniscaf, node-13 TCCCGCAAGTCGGGAGGTTCGCTAAAACGC 20170608, cc6hb v4-250 nm 16 component square, 469 CTCGCGTTCTTA default 6hb, miniscaf, node-14 GGTGCAAAATAGCAACTAATCTTGATTTAA 20170608, cc6hb v4-250 nm 16 component square, 470 GGCTTCAAAACC default 6hb, miniscaf, node-15 GCAAATTAGGCTCTGGAAAGACGCTCGTTAGC 20170608, cc6hb v4-250 nm 16 component square, 471 GTTGGTAAGATTCAGGATAAAATTGTAGCTG default 6hb, miniscaf, node-16 ATTCTCAATTAGCGTGGACCGTTGAGCGTTG cc6hbv3_miniscaf_10s_n4 472 GCTTTATACTG TGCCTCTGCCTGAACCACCATTTGGCGTTGT cc6hbv3_miniscaf_10s_n5 473 TAAATATGGCG CTTTTGTCGGTTCGGGCTATTCTCTTATTACT cc6hbv3_miniscaf_10s_n6 474 GGCTCGAAAA AACATGTTGTTAGTGGACTCTGTCTGGACAG cc6hbv3_miniscaf_10s_n7 475 AATTACTTTAC ACTTATCTATTACGGTTTTTCAGGCGCGTTC cc6hbv3_miniscaf_10s_n8 476 TGCATTAGCTG TAATACCATAAATCAAAAATCAGTTGATAA cc6hbv3_yellow_term_10s_n4 477 GA AGGCTACGAGGCATAGTAAGAGCAAACAGT cc6hbv3_yellow_term_10s_n5 478 TC ACCGTGAGATGGTTTAATTTCAAAGATACAT cc6hbv3_yellow_term_10s_n6 479 A AACACGGTCAATCATAAGGGAACCAGAACG cc6hbv3_yellow_term_10s_n7 480 AG AATATCCATTAAACGGGTAAAATACTTAGC cc6hbv3_yellow_term_10s_n8 481 CG

TABLE 3 Exemplary 440 nm Six-helix Bundle SEQ ID Sequence Comment NO: AACGGCATCTCCGTGAGCCTCCTCACAGAG 6hb_440 nm, start 0[76], end 5[62] 482 CCTGGGGTGCCT GGCAGCACCCATCCCTTACACTGGTGTGGTT 6hb_440 nm, start 0[118], end 5[104] 483 GCGCTCACTGC AAATCCCGTGGTCTGGTCAGCAGCAACCCC 6hb_440 nm, start 0[160], end 5[146] 484 AGCTGCATTAAT GAGCCGCCAAGCAGTTGGGCGGTTGTGTTTT 6hb_440 nm, start 0[202], end 5[188] 485 GCGTATTGGGC GGCACCGCTAAAACGACGGCCAGTGCCAAG 6hb_440 nm, start 0[244], end 5[230] 486 ACGGGCAACAGC CGCGTCTGGGCCTCAGGAAGATCGCACTAG 6hb_440 nm, start 0[286], end 5[272] 487 AGTTGCAGCAAG GGAGCAAACTTTTAACCAATAGGAACGCGA 6hb_440 nm, start 0[328], end 5[314] 488 AAATCCTGTTTG GCAAGGATATACAAAGGCTATCAGGTCATT 6hb_440 nm, start 0[370], end 5[356] 489 ATAAATCAAAAG CTGTTTAGCTAATACTTTTGCGGGAGAATCC 6hb_440 nm, start 0[412], end 5[398] 490 AGTTTGGAACA TACCTTTAAACCATTAGATACATTTCGCCAA 6hb_440 nm, start 0[454], end 5[440] 491 CGTCAAAGGGC ATCCCCCTCGGAAGCAAACTCCAACAGGAC 6hb_440 nm, start 0[496], end 5[482] 492 TACGTGAACCAT ACCACATTCCAATACTGCGGAATCGTCAGT 6hb_440 nm, start 0[538], end 5[524] 493 GCCGTAAAGCAC TGCCCTGACGGTAGAAAGATTCATCAGTATT 6hb_440 nm, start 0[580], end 5[566] 494 TAGAGCTTGAC CGCGACCTGCGTAACAAAGCTGCTCATTGG 6hb_440 nm, start 0[622], end 5[608] 495 AAGGGAAGAAAG ACAGAGGCTTTGTATCATCGCCTGATAAAA 6hb_440 nm, start 0[664], end 5[650] 496 GTGTAGCGGTCA CCTTTAATTAAAGACAGCATCGGAACGAGC 6hb_440 nm, start 0[706], end 5[692] 497 TTAATGCGCCGC TAGCATTCCTGAAAATCTCCAAAAAAAACG 6hb_440 nm, start 0[748], end 5[734] 498 AGCACGTATAAC GATAAGTGCGAGTTTCGTCACCAGTACAAG 6hb_440 nm, start 0[790], end 5[776] 499 CTAAACAGGAGG AGTAAGCGTTAGGATTAGCGGGGTTTTGGT 6hb_440 nm, start 0[832], end 5[818] 500 ACGCCAGAATCC CACCGGAACAATGGAAAGCGCAGTCTCTCA 6hb_440 nm, start 0[874], end 5[860] 501 CCGAGTAAAAGA CAGCAAAATTTTCATAATCAAAATCACCTA 6hb_440 nm, start 0[916], end 5[902] 502 GCAATACTTCTT TAAAGGTGGCGTCACCGACTTGAGCCATTA 6hb_440 nm, start 0[958], end 5[944] 503 GAAGAACTCAAA ATTGAGTTAGCAGTATGTTAGCAAACGTCA 6hb_440 nm, start 0[1000], end 5[986] 504 ATATTACCGCCA ATTTGCCAGTGAGCGCTAATATCAGAGAAA 6hb_440 nm, start 0[1042], end 5[1028] 505 ATACCTACATTT TTTCATCGTTACCAACGCTAACGAGCGTTTA 6hb_440 nm, start 0[1084], end 5[1070] 506 CATTGGCAGAT CCAGACGACCGCACTCATCGAGAACAAGGG 6hb_440 nm, start 0[1126], end 5[1112] 507 ACATTCTGGCCA AATAAACACATAAAGTACCGACAAAAGGGC 6hb_440 nm, start 0[1168], end 5[1154] 508 GTAAGAATACGT ATTTATCAAACCGACCGTGTGATAAATATA 6hb_440 nm, start 0[1210], end 5[1196] 509 GTCTTTAATGCG AGATGATGATTAGATTAAGACGCTGAGATA 6hb_440 nm, start 0[1252], end 5[1238] 510 AAAATACCGAAC AGGGTTAGACGAATTATTCATTTCAATTTGA 6hb_440 nm, start 0[1294], end 5[1280] 511 GGCGGTCAGTA AGAAGTATTCTGATTGTTTGGATTATACGAG 6hb_440 nm, start 0[1336], end 5[1322] 512 AGCCAGCAGCA TCATGGTCATAGCCGTGCCTGTTCTTCGCGA 6hb_440 nm, start 1[38], end 1[79] 513 GATGCCGGGTT ACCTGCAGCCAGCTCTTTGCTCGTCATAAAG 6hb_440 nm, start 1[80], end 1[121] 514 TCGGTGGTGCC ATCCCACGCAACCAACGTCAGCGTGGTGCT 6hb_440 nm, start 1[122], end 1[163] 515 AAAAAAAGCCGC ACAGGCGGCCTTTTCTGCTCATTTGCCGCCC 6hb_440 nm, start 1[164], end 1[205] 516 GGGAACGGATA ACCTCACCGGAAACCCAGTCACGACGTTGT 6hb_440 nm, start 1[206], end 1[247] 517 TCTGGTGCCGGA AACCAGGCAAAGCGGACGACGACAGTATCG 6hb_440 nm, start 1[248], end 1[289] 518 CCTTCCTGTAGC CAGCTTTCATCAATGTTAAATCAGCTCATTA 6hb_440 nm, start 1[290], end 1[331] 519 AGAGAATCGAT GAACGGTAATCGTGCTATTTTTGAGAGATCA 6hb_440 nm, start 1[332], end 1[373] 520 AAATTTTTAGA ACCCTCATATATTAAAACATTATGACCCTGT 6hb_440 nm, start 1[374], end 1[415] 521 ATATTTTCATT TGGGGCGCGAGCTCGAGTAGATTTAGTTTGT 6hb_440 nm, start 1[416], end 1[457] 522 TGCTCCTTTTG ATAAGAGGTCATTTCAAAGCGAACCAGACC 6hb_440 nm, start 1[458], end 1[499] 523 AAATGCTTTAAA CAGTTCAGAAAACTTAGACTGGATAGCGTC 6hb_440 nm, start 1[500], end 1[541] 524 AACTAATGCAGA TACATAACGCCAAGGAACAACATTATTACA 6hb_440 nm, start 1[542], end 1[583] 525 GAGAAACACCAG AACGAGTAGTAAATTCATTACCCAAATCAA 6hb_440 nm, start 1[584], end 1[625] 526 CTCCATGTTACT TAGCCGGAACGAGCAAAGTACAACGGAGAT 6hb_440 nm, start 1[626], end 1[667] 527 TTGAGGACTAAA GACTTTTTCATGACGTCACCCTCAGCAGCGG 6hb_440 nm, start 1[668], end 1[709] 528 TATCGGTTTAT CAGCTTGCTTTCGTAATAATTTTTTCACGTA 6hb_440 nm, start 1[710], end 1[751] 529 CAGACAGCCCT CATAGTTAGCGTACCATGTACCGTAACACTC 6hb_440 nm, start 1[752], end 1[793] 530 GTCGAGAGGGT TGATATAAGTATACTCCTCAAGAGAAGGAT 6hb_440 nm, start 1[794], end 1[835] 531 CATACATGGCTT TTGATGATACAGGATCCTCATTAAAGCCAG 6hb_440 nm, start 1[836], end 1[877] 532 CGCCTCCCTCAG AGCCGCCACCCTCATTAGCGTTTGCCATCTC 6hb_440 nm, start 1[878], end 1[919] 533 ACCAGTAGCAC CATTACCATTAGCTAAAGGTGAATTATCACC 6hb_440 nm, start 1[920], end 1[961] 534 AACATATAAAA GAAACGCAAAGACTTAAGACTCCTTATTAC 6hb_440 nm, start 1[962], end 1[1003] 535 AGCCCAATAATA AGAGCAAGAAACACAAAGTCAGAGGGTAA 6hb_440 nm, start 1[1004], end 1[1045] 536 TTTACAAAATAAA CAGCCATATTATTAATTTTATCCTGAATCTA 6hb_440 nm, start 1[1046], end 1[1087] 537 GGAATCATTAC CGCGCCCAATAGCGGTATTAAACCAAGTAC 6hb_440 nm, start 1[1088], end 1[1129] 538 GACAATAAACAA CATGTTCAGCTAAGCCAGTAATAAGAGAAT 6hb_440 nm, start 1[1130], end 1[1171] 539 CGGAATCATAAT TACTAGAAAAAGCATTTAATGGTTTGAAAT 6hb_440 nm, start 1[1172], end 1[1213] 540 AATCATAGGTCT GAGAGACTACCTTGAAAACATAGCGATAGC 6hb_440 nm, start 1[1214], end 1[1255] 541 AACAAACATCAA GAAAACAAAATTAACAAAATCGCGCAGAG 6hb_440 nm, start 1[1256], end 1[1297] 542 GACCTACCATATC AAAATTATTTGCAAATTCATCAATATAATCA 6hb_440 nm, start 1[1298], end 1[1343] 543 GACTTTACAAACAAT TCGACAACTCTAACAACTAATCGTCAATAG 6hb_440 nm, start 1[1344], end 5[1364] 544 ATAATGAACCTCAAATATC TCTGCCAGCACGTGTTTCCTGTGTGCCGCTC 6hb_440 nm, start 2[62], end 3[45] 545 AC TGGGTAAAGGTTGGTGCCGGTGCCCCCTGC 6hb_440 nm, start 2[104], end 2[63] 546 ATACCGGGGGTT CCGGACTTGTAGAGCTTACGGCTGGAGGTG 6hb_440 nm, start 2[146], end 2[105] 547 TGCGGCTGGTAA CAAACTTAAATTAGTGATGAAGGGTAAAGT 6hb_440 nm, start 2[188], end 2[147] 548 TAACGGAACGTG CGCCAGGGTTTTCAATCGGCGAAACGTACA 6hb_440 nm, start 2[230], end 2[189] 549 GAAACAGCGGAT GCCAGTTTGAGGGCCATTCGCCATTCAGGCT 6hb_440 nm, start 2[272], end 2[231] 550 AAGTTGGGTAA GCATTAAATTTTCATTAAATGTGAGCGAGTA 6hb_440 nm, start 2[314], end 2[273] 551 ACCGTGCATCT CCGGAGAGGGTAAAAACTAGCATGTCAATC 6hb_440 nm, start 2[356], end 2[315] 552 TTGTTAAAATTC TCGGTTGTACCATTAAATGCAATGCCTGAGG 6hb_440 nm, start 2[398], end 2[357] 553 ATAAATTAATG CAATTCTGCGAAGAAAAGGTGGCATCAATT 6hb_440 nm, start 2[440], end 2[399] 554 CATAAAGCTAAA TTAATTCGAGCTTTTGCGGATGGCTTAGAGA 6hb_440 nm, start 2[482], end 2[441] 555 CAGTTGATTCC ATAGTAAAATGTGAGAATGACCATAAATCA 6hb_440 nm, start 2[524], end 2[483] 556 AAATATCGCGTT AAACGAACTAACAAGGAATTACGAGGCATA 6hb_440 nm, start 2[566], end 2[525] 557 CCAGAGGGGGTA AAGAACCGGATATTGGGCTTGAGATGGTTT 6hb_440 nm, start 2[608], end 2[567] 558 TCTACGTTAATA CCAAGCGCGAAAGCGCAGACGGTCAATCAT 6hb_440 nm, start 2[650], end 2[609] 559 AGTAATCTTGAC CTTTTGCGGGATGGAAGTTTCCATTAAACGC 6hb_440 nm, start 2[692], end 2[651] 560 CAGCGATTATA AGGAATTGCGAAAGGTGAATTTCTTAAACA 6hb_440 nm, start 2[734], end 2[693] 561 AGTTAAAGGCCG CCCAATAGGAACACGATCTAAAGTTTTGTC 6hb_440 nm, start 2[776], end 2[735] 562 AGGAACAACTAA AAGAGGCTGAGAGCCCGGAATAGGTGTATC 6hb_440 nm, start 2[818], end 2[777] 563 AGGGATAGCAAG ACAAACAAATAAAGTGTACTGGTAATAAGT 6hb_440 nm, start 2[860], end 2[819] 564 CATGAAAGTATT CATAGCCCCCTTAGAACCGCCACCCTCAGA 6hb_440 nm, start 2[902], end 2[861] 565 GCCTTGATATTC GAAATTATTCATAAGGCCGGAAACGTCACC 6hb_440 nm, start 2[944], end 2[903] 566 GGCATTTTCGGT GAACTGGCATGAACCACGGAATAAGTTTAT 6hb_440 nm, start 2[986], end 2[945] 567 TAAATATTGACG GAACACCCTGAAATGAAATAGCAATAGCTA 6hb_440 nm, start 2[1028], end 2[987] 568 GAATACCCAAAA GCACCCAGCTACTATCCCAATCCAAATAAG 6hb_440 nm, start 2[1070], end 2[1029] 569 GGAGAATTAACT ATTCCAAGAACGAAGCAAATCAGATATAGA 6hb_440 nm, start 2[1112], end 2[1071] 570 AGTTGCTATTTT AGGCATTTTCGATGCAGAACGCGCCTGTTTT 6hb_440 nm, start 2[1154], end 2[1113] 571 CTTTCCTTATC CTTCTGACCTAACTGTTTAGTATCATATGCT 6hb_440 nm, start 2[1196], end 2[1155] 572 AATTTAGGCAG CTTAGAATCCTTTTTAACCTCCGGCTTAGGA 6hb_440 nm, start 2[1238], end 2[1197] 573 GTTAATTTCAT GAATACCAAGTTATTACATTTAACAATTTCA 6hb_440 nm, start 2[1280], end 2[1239] 574 ATTAATTTTCC TCAGATGATGGCCGTAAAACAGAAATAAAG 6hb_440 nm, start 2[1322], end 2[1281] 575 CCTGATTGCTTT TCTTTAGGAGCACGTATTAAATCCTTTGCCT 6hb_440 nm, start 2[1364], end 2[1323] 576 ATTCCTGATTA AATTCCACACAAGGGCCGTTTTCACGGTCAT 6hb_440 nm, start 3[46], end 3[87] 577 CAGACGATCCA GCGCAGTGTCACCCGGGTCACTGTTGCCCTC 6hb_440 nm, start 3[88], end 3[129] 578 CAGCATCAGCG GGGTCATTGCAGGCCAGAGCACATCCTCAT 6hb_440 nm, start 3[130], end 3[171] 579 AAACGATGCTGA TTGCCGTTCCGGACGGAAAAAGAGACGCAG 6hb_440 nm, start 3[172], end 3[213] 580 CGCCATGTTTAC CAGTCCCGGAATATGTGCTGCAAGGCGATT 6hb_440 nm, start 3[214], end 3[255] 581 GCGCAACTGTTG GGAAGGGCGATCGTAGATGGGCGCATCGTA 6hb_440 nm, start 3[256], end 3[297] 582 ACAACCCGTCGG ATTCTCCGTGGGTTGTAAACGTTAATATTAT 6hb_440 nm, start 3[298], end 3[339] 583 ATGTACCCCGG TTGATAATCAGAATTCAACCGTTCTAGCTTA 6hb_440 nm, start 3[340], end 3[381] 584 ATGTGTAGGTA AAGATTCAAAAGGCAATAAAGCCTCAGAGC 6hb_440 nm, start 3[382], end 3[423] 585 TACTAATAGTAG TAGCATTAACATAAGTTTCATTCCATATACT 6hb_440 nm, start 3[424], end 3[465] 586 TAATTGCTGAA TATAATGCTGTAGAAGCCCGAAAGACTTCA 6hb_440 nm, start 3[466], end 3[507] 587 AAATCAGGTCTT TACCCTGACTATTTGCAAAAGAAGTTTTGGT 6hb_440 nm, start 3[508], end 3[549] 588 AAGAGCAACAC TATCATAACCCTGACGTTGGGAAGAAAAAA 6hb_440 nm, start 3[550], end 3[591] 589 ATTTCAACTTTA ATCATTGTGAATGGCTGACCTTCATCAAGAA 6hb_440 nm, start 3[592], end 3[633] 590 GGGAACCGAAC TGACCAACTTTGACACTCATCTTTGACCCGG 6hb_440 nm, start 3[634], end 3[675] 591 TAAAATACGTA ATGCCACTACGACGCTGAGGCTTGCAGGGG 6hb_440 nm, start 3[676], end 3[717] 592 CTTGATACCGAT AGTTGCGCCGACGCGGAGTGAGAATAGAAG 6hb_440 nm, start 3[718], end 3[759] 593 TCTTTCCAGACG TTAGTAAATGAACCACCACCCTCATTTTCAC 6hb_440 nm, start 3[760], end 3[801] 594 CGTACTCAGGA GGTTTAGTACCGAACCTATTATTCTGAAATT 6hb_440 nm, start 3[802], end 3[843] 595 TAACGGGGTCA GTGCCTTGAGTAGGCAGGTCAGACGATTGG 6hb_440 nm, start 3[844], end 3[885] 596 CCACCACCCTCA GAGCCGCCACCATGTAGCGCGTTTTCATCA 6hb_440 nm, start 3[886], end 3[927] 597 ATGAAACCATCG ATAGCAGCACCGGATTGAGGGAGGGAAGGT 6hb_440 nm, start 3[928], end 3[969] 598 TTGTCACAATCA ATAGAAAATTCAGAAACGCAATAATAACGT 6hb_440 nm, start 3[970], end 3[1011] 599 CTTACCGAAGCC CTTTTTAAGAAAGGGAAGCGCATTAGACGA 6hb_440 nm, start 3[1012], end 3[1053] 600 AACGATTTTTTG TTTAACGTCAAAAGCCTTAAATCAAGATTA 6hb_440 nm, start 3[1054], end 3[1095] 601 GGCTTATCCGGT ATTCTAAGAACGCAATCAATAATCGGCTGA 6hb_440 nm, start 3[1096], end 3[1137] 602 TCAACAATAGAT AAGTCCTGAACATTAACAACGCCAACATGG 6hb_440 nm, start 3[1138], end 3[1179] 603 TTATACAAATTC TTACCAGTATAATTTTTCAAATATATTTTTTG 6hb_440 nm, start 3[1180], end 3[1221] 604 GGTTATATAA CTATATGTAAATTGTAAATCGTCGCTATTAT 6hb_440 nm, start 3[1222], end 3[1263] 605 TTGAATTACCT TTTTTAATGGAAAAACAATAACGGATTCGA 6hb_440 nm, start 3[1264], end 3[1305] 606 AATTGCGTAGAT TTTCAGGTTTAAGAGCGGAATTATCATCACG 6hb_440 nm, start 3[1306], end 3[1347] 607 AACGTTATTAA TTTTAAAAGTTTAAAGGAATTGAGTAAAAT 6hb_440 nm, start 3[1348], end 2[1365] 608 A CGGAAGCATAAAGTGTAATTGAGGATCCCC 6hb_440 nm, start 4[48], end 0[35] 609 GG GTGCACTCTGTGGTCTCACATTAATTGCTTC 6hb_440 nm, start 4[90], end 0[77] 610 AGCAAATCGTT CACTCAATCCGCCGGGAAACCTGTCGTGGC 6hb_440 nm, start 4[132], end 0[119] 611 AAGAATGCCAAC TCCGTTTTTTCGTCGCGGGGAGAGGCGGAC 6hb_440 nm, start 4[174], end 0[161] 612 ATCGACATAAAA ATAGACTTTCTCCGTCTTTTCACCAGTGAGC 6hb_440 nm, start 4[216], end 0[203] 613 TTTCAGAGGTG CTCTTCGCTATTACCGCCTGGCCCTGAGCCA 6hb_440 nm, start 4[258], end 0[245] 614 GCCAGCTTTCC CGGATTGACCGTAATTGCCCCAGCAGGCCA 6hb_440 nm, start 4[300], end 0[287] 615 TCAAAAATAATT AAAACAGGAAGATTATCGGCAAAATCCCTT 6hb_440 nm, start 4[342], end 0[329] 616 GCCTGAGAGTCT GGCCGGAGACAGTCGGGTTGAGTGTTGTGC 6hb_440 nm, start 4[384], end 0[371] 617 CTTTATTTCAAC CATACAGGCAAGGCAAGAACGTGGACTCAA 6hb_440 nm, start 4[426], end 0[413] 618 ATGGTCAATAAC GTTTTAAATATGCACAGGGCGATGGCCCTC 6hb_440 nm, start 4[468], end 0[455] 619 AGGATTAGAGAG AAGCAAAGCGGATTTTTTTGGGGTCGAGTA 6hb_440 nm, start 4[510], end 0[497] 620 AATATTCATTGA GACGACGATAAAAAAAAGGGAGCCCCCGT 6hb_440 nm, start 4[552], end 0[539] 621 GAGATTTAGGAAT CGATTTTAAGAACTAACGTGGCGAGAAACA 6hb_440 nm, start 4[594], end 0[581] 622 GTGAATAAGGCT AGATGAACGGTGTAGCTAGGGCGCTGGCAT 6hb_440 nm, start 4[636], end 0[623] 623 TGTGTCGAAATC CCTAAAACGAAAGAACCACACCCGCCGCGG 6hb_440 nm, start 4[678], end 0[665] 624 GTAGCAACGGCT AACCATCGCCCACGTATGGTTGCTTTGAGGC 6hb_440 nm, start 4[720], end 0[707] 625 TCCAAAAGGAG TGGGATTTTGCTAAAGAATCAGAGCGGGAA 6hb_440 nm, start 4[762], end 0[749] 626 CTACAACGCCTG GAACCGCCACCCTCTTTAGACAGGAACGCT 6hb_440 nm, start 4[804], end 0[791] 627 CAGTACCAGGCG GTATAAACAGTTAAATAATCAGTGAGGCGA 6hb_440 nm, start 4[846], end 0[833] 628 ATTTACCGTTCC CCAGAGCCGCCGCCCAAATTAACCGTTGGG 6hb_440 nm, start 4[888], end 0[875] 629 AACCAGAGCCAC GCGACAGAATCAAGATCACTTGCCTGAGTT 6hb_440 nm, start 4[930], end 0[917] 630 GGGAATTAGAGC CCAGCGCCAAAGACGGTAATATCCAGAAAG 6hb_440 nm, start 4[972], end 0[959] 631 AAAATACATACA ATAGCCGAACAAAGAAAAACGCTCATGGGA 6hb_440 nm, start 4[1014], end 0[1001] 632 TAACCCACAAGA AGCAGCCTTTACAGCTGAAATGGATTATCTT 6hb_440 nm, start 4[1056], end 0[1043] 633 TCCAGAGCCTA TTAGCGAACCTCCCACCAGTAATAAAAGCA 6hb_440 nm, start 4[1098], end 0[1085] 634 AGCCGTTTTTAT ATATCCCATCCTAACTTCTGACCTGAAATAA 6hb_440 nm, start 4[1140], end 0[1127] 635 AGTAATTCTGT TCAACAGTAGGGCTTTTTGAATGGCTATAGG 6hb_440 nm, start 4[1182], end 0[1169] 636 CGTTAAATAAG AATCCAATCGCAAGTAAAACATCGCCATAG 6hb_440 nm, start 4[1224], end 0[1211] 637 AGTCAATAGTGA AAATCAATATATGTAGATAAAACAGAGGAC 6hb_440 nm, start 4[1266], end 0[1253] 638 CTGAGCAAAAGA AATATACAGTAACAAACAGTGCCACGCTTT 6hb_440 nm, start 4[1308], end 0[1295] 639 CTGAATAATGGA TATCATTTTGCGGAAGCATCACCTTGCTACA 6hb_440 nm, start 4[1350], end 0[1337] 640 TTTGAGGATTT AATGAGTGAGCTAAGCTGCGGCCAGAATGC 6hb_440 nm, start 5[63], end 4[49] 641 GGCCATACGAGC CCGCTTTCCAGTCGGGCGCGGTTGCGGTATG 6hb_440 nm, start 5[105], end 4[91] 642 AGTGCGCGCCT GAATCGGCCAACGCTCGTCGCTGGCAGCCT 6hb_440 nm, start 5[147], end 4[133] 643 CCGGCGCTTTCG GCCAGGGTGGTTTTTGGTGAAGGGATAGCT 6hb_440 nm, start 5[189], end 4[175] 644 CTCCAAACGCGG TGATTGCCCTTCACGCCAGCTGGCGAAAGG 6hb_440 nm, start 5[231], end 4[217] 645 GGGTTGTGAGAG CGGTCCACGCTGGTTGGGATAGGTCACGTT 6hb_440 nm, start 5[273], end 4[259] 646 GGTGGTGCGGGC ATGGTGGTTCCGAAGTATAAGCAAATATTT 6hb_440 nm, start 5[315], end 4[301] 647 AAAAACAAACGG AATAGCCCGAGATAAAATCACCATCAATAT 6hb_440 nm, start 5[357], end 4[343] 648 GATAAAGCCCCA AGAGTCCACTATTAAAAGAATTAGCAAAAT 6hb_440 nm, start 5[399], end 4[385] 649 TAAGGTGAGAAA GAAAAACCGTCTATACTAAAGTACGGTGTC 6hb_440 nm, start 5[441], end 4[427] 650 TGGCCAATAAAT CACCCAAATCAAGTGCATCAAAAAGATTAA 6hb_440 nm, start 5[483], end 4[469] 651 GAGGCTCAACAT TAAATCGGAACCCTCCAAAATAGCGAGAGG 6hb_440 nm, start 5[525], end 4[511] 652 CTTTATAGTCAG GGGGAAAGCCGGCGGGCTCATTATACCAGT 6hb_440 nm, start 5[567], end 4[553] 653 CAGCGTTTACCA CGAAAGGAGCGGGCCAGACCAGGCGCATA 6hb_440 nm, start 5[609], end 4[595] 654 GGCTTACCTTATG CGCTGCGCGTAACCGGCAAAAGAATACACT 6hb_440 nm, start 5[651], end 4[637] 655 AAAAAAGAGGAC TACAGGGCGCGTACCATAACCGATATATTC 6hb_440 nm, start 5[693], end 4[679] 656 GGTAGGCACCAA GTGCTTTCCTCGTTACAACTTTCAACAGTTT 6hb_440 nm, start 5[735], end 4[721] 657 CAAATGACAAC CCGATTAAAGGGATAGAACCGCCACCCTCA 6hb_440 nm, start 5[777], end 4[763] 658 GAGTTTTCTGTA TGAGAAGTGTTTTTTGCCCCCTGCCTATTTC 6hb_440 nm, start 5[819], end 4[805] 659 GGCCACCCTCA GTCTGTCCATCACGAGCATTGACAGGAGGT 6hb_440 nm, start 5[861], end 4[847] 660 TGAACAGTGCCC TGATTAGTAATAACTTTGCCTTTAGCGTCAG 6hb_440 nm, start 5[903], end 4[889] 661 ACGAACCACCA CTATCGGCCTTGCTAAAAGGGCGACATTCA 6hb_440 nm, start 5[945], end 4[931] 662 ACCTAATCAGTA GCCATTGCAACAGGTTACCAGAAGGAAACC 6hb_440 nm, start 5[987], end 4[973] 663 GAGTATGGTTTA TGACGCTCAATCGTAGAGAATAACATAAAA 6hb_440 nm, start 5[1029], end 4[1015] 664 ACAAGTAAGCAG TCACCAGTCACACGGACTTGCGGGAGGTTT 6hb_440 nm, start 5[1071], end 4[1057] 665 TGAAATGAAAAT ACAGAGATAGAACCTTTACGAGCATGTAGA 6hb_440 nm, start 5[1113], end 4[1099] 666 AACCGAGGCGTT GGCACAGACAATATTAATTGAGAATCGCCA 6hb_440 nm, start 5[1155], end 4[1141] 667 TATAGAAAAATA CGAACTGATAGCCCACAAAGAACGCGAGAA 6hb_440 nm, start 5[1197], end 4[1183] 668 AACAGCCAACGC GAACCACCAGCAGAGAGTGAATAACCTTGC 6hb_440 nm, start 5[1239], end 4[1225] 669 TTCGCTGATGCA TTAACACCGCCTGCGTACCTTTTACATCGGG 6hb_440 nm, start 5[1281], end 4[1267] 670 AGACAGTACAT AATGAAAAATCTAAACAAAGAAACCACCAG 6hb_440 nm, start 5[1323], end 4[1309] 671 AAGCGTCAGATG AAACCCTCAATCAAGTTGGCAAATCAACAG 6hb_440 nm, start 5[1365], end 4[1351] 672 TTGGAGTAACAT CGCTGGTTGGGATAGGTCACGTTGGTGGTG 6hb_440 nm, 7 bp socket end distal to queen, start 673 CGGGC 5[280], end 4[259] TTCCGAAGTATAAGCAAATATTTAAAAACA 6hb_440 nm, 7 bp socket end distal to queen, start 674 AACGG 5[322], end 4[301] CGAGATAAAATCACCATCAATATGATAAAG 6hb_440 nm, 7 bp socket end distal to queen, start 675 CCCCA 5[364], end 4[343] ACTATTAAAAGAATTAGCAAAATTAAGGTG 6hb_440 nm, 7 bp socket end distal to queen, start 676 AGAAA 5[406], end 4[385] CGTCTATACTAAAGTACGGTGTCTGGCCAAT 6hb_440 nm, 7 bp socket end distal to queen, start 677 AAAT 5[448], end 4[427] TGGTTGGGATAGGTCACGTTGGTGGTGCGG 6hb_440 nm, 10 bp socket end distal to queen, start 678 GC 5[283], end 4[259] CGAAGTATAAGCAAATATTTAAAAACAAAC 6hb_440 nm, 10 bp socket end distal to queen, start 679 GG 5[325], end 4[301] GATAAAATCACCATCAATATGATAAAGCCC 6hb_440 nm, 10 bp socket end distal to queen, start 680 CA 5[367], end 4[343] ATTAAAAGAATTAGCAAAATTAAGGTGAGA 6hb_440 nm, 10 bp socket end distal to queen, start 681 AA 5[409], end 4[385] CTATACTAAAGTACGGTGTCTGGCCAATAA 6hb_440 nm, 10 bp socket end distal to queen, start 682 AT 5[451], end 4[427]

All references, patents and patent applications disclosed herein are incorporated by reference with respect to the subject matter for which each is cited, which in some cases may encompass the entirety of the document.

The indefinite articles “a” and “an,” as used herein in the specification and in the claims, unless clearly indicated to the contrary, should be understood to mean “at least one.”

It should also be understood that, unless clearly indicated to the contrary, in any methods claimed herein that include more than one step or act, the order of the steps or acts of the method is not necessarily limited to the order in which the steps or acts of the method are recited.

In the claims, as well as in the specification above, all transitional phrases such as “comprising,” “including,” “carrying,” “having,” “containing,” “involving,” “holding,” “composed of,” and the like are to be understood to be open-ended, i.e., to mean including but not limited to. Only the transitional phrases “consisting of” and “consisting essentially of” shall be closed or semi-closed transitional phrases, respectively, as set forth in the United States Patent Office Manual of Patent Examining Procedures, Section 2111.03. 

What is claimed is:
 1. A composition, comprising: (a) a nucleating nucleic acid nanostructure; (b) a first layer of parallel elongated nucleic acid nanostructures stably bound to the nucleating nanostructure of (a); and (c) a second layer of parallel elongated nucleic acid nanostructures stably bound to the elongated nanostructures of (b) and rotated at an angle relative to the parallel elongated nanostructures of (b), wherein a single elongated nanostructure of (b) binds to multiple elongated nanostructures of (c), each through a single cooperative binding site.
 2. The composition of claim 1 further comprising (d) a third layer of parallel elongated nanostructures stably bound to the elongated nanostructures of (c) and rotated at an angle relative to the parallel elongated nanostructures of (c).
 3. The composition of claim 1 or 2, wherein the second layer is rotated by 10 degrees to 170 degrees relative to the first layer.
 4. The composition of claim 3, wherein the second layer is rotated by 90 degrees relative to the first layer.
 5. The composition of any one of claims 1-4, wherein the third layer is rotated by 10 degrees to 170 degrees relative to the second layer.
 6. The composition of claim 5, wherein the third layer is rotated by 90 degrees.
 7. The composition of any one of claims 1-6, wherein the third layer is parallel to the first layer.
 8. The composition of any one of claims 1-7, wherein the nucleic acid of (a) is DNA.
 9. The composition of any one of claims 1-8, wherein the nucleic acid of (b) and/or (c) is DNA.
 10. The composition of claim 8 or 9, wherein the nanostructures of (a), (b) and/or (c) comprise a nucleic acid scaffold strand bound to multiple nucleic acid staple strands that are shorter than the nucleic acid scaffold strand.
 11. The composition of claim 10, wherein the length of the nucleic acid scaffold strand is longer than 1 kilobase, and each of the nucleic acid staple strands is shorter than 200 nucleotides.
 12. The composition of any one of claims 8-11, wherein the nucleating nanostructure of (b) and/or (c) comprise DNA nanorods.
 13. The composition of any one of claims 8-12, wherein the nucleating nanostructure of (b) and/or (c) comprise DNA slats.
 14. A method, comprising: combining in reaction buffer (a) a nucleating nucleic acid nanostructure; (b) a first subset of elongated nucleic acid nanostructures that stably bind to the nucleating nanostructure of (a); and (c) a second subset of elongated nucleic acid nanostructures that stably bind to the elongated nanostructures of (b); and incubating the reaction buffer comprising (a), (b) and (c) under conditions that result in (i) stable binding of the elongated nanostructures of (b) to the nucleating nanostructure of (a) to form a first layer of parallel elongated nanostructures, and (ii) binding of the elongated nanostructures of (c) to the elongated nanostructures of the first layer to form a second layer of parallel elongated nanostructures that is rotated at an angle relative to the first layer, wherein a single elongated nanostructure of (b) binds to multiple elongated nanostructures of (c), each through a single cooperative binding site.
 15. The method of claim 14, wherein the second layer is rotated by 10 degrees to 170 degrees relative to the first layer.
 16. The method of claim 15, wherein the second layer is rotated by 90 degrees relative to the first layer.
 17. The method of any one of claims 14-16, wherein the nucleic acid of (a) is DNA.
 18. The method of any one of claims 14-17, wherein the nucleic acid of (b) and/or (c) is DNA.
 19. The method of claim 17 or 18, wherein the nanostructures of (a), (b) and/or (c) comprise a nucleic acid scaffold strand bound to multiple nucleic acid staple strands that are shorter than the nucleic acid scaffold strand.
 20. The method of claim 19, wherein the length of the nucleic acid scaffold strand is longer than 1 kilobase, and each of the nucleic acid staple strands is shorter than 200 nucleotides.
 21. The method of any one of claims 17-20, wherein the nucleating nanostructure of (b) and/or (c) comprise DNA nanorods.
 22. The method of any one of claims 17-21 wherein the nucleating nanostructure of (b) and/or (c) comprise DNA slats.
 23. A crisscross nucleic acid nanostructure, comprising: a first nanorod comprising a first plug strand and a second plug strand; a second nanorod comprising a third plug strand and a fourth plug strand, wherein the second nanorod is parallel to the first nanorod; a third nanorod comprising a fifth plug strand complementary to and bound to the first plug strand and a sixth plug strand complementary to and bound to the second plug strand; a fourth nanorod comprising a seventh plug strand complementary to and bound to the third plug strand and an eighth plug strand complementary to and bound to the fourth plug strand, wherein the third nanorod is parallel to the fourth nanorod.
 24. A crisscross nucleic acid nanostructure, comprising: (a) a first plurality of nanorods aligned parallel to each other; and (b) a second plurality of nanorods aligned parallel to each other, wherein the nanorods of the first plurality are bound to and perpendicular to the nanorods of the second plurality, a single nanorod of (b) binds to multiple nanorods of (a), each through a single cooperative binding site, and single nanorod of (a) binds to multiple nanorods of (b), each through a single cooperative binding site.
 25. The crisscross nucleic acid nanostructure of claim 23 or 24, wherein each nanorod comprises DNA.
 26. The crisscross nucleic acid nanostructure of claim 25, wherein each nanorod comprises a 6-helix DNA bundle.
 27. A crisscross nucleic acid slat, comprising: (a) a first plurality of at least four nucleic acid strands aligned parallel to each other, each strand of the first plurality having a length of 15-30 nucleotides; and (b) a second plurality of at least four nucleic acid strands aligned parallel to each, each strand of the second plurality having a length of 15-30 nucleotides, wherein the at least four nucleic acid strands of the first plurality are bound to and perpendicular to the at least four nucleic acid strands of the second plurality, a single nucleic acid strand of (b) binds to multiple nucleic acid strands of (a), each through a single cooperative binding site, and a single nucleic acid strand of (a) binds to multiple nucleic acid strands of (b), each through a single cooperative binding site.
 28. A nucleic acid nanostructure, comprising a nucleic acid scaffold strand folded into multiple stacked parallel loops that are bound to at least one crisscross nucleic acid slat of claim
 27. 29. A nucleic acid nanostructure comprising a stack of parallel nucleic acid loops bound to a plurality of nucleic acid slats of claim
 27. 30. A method of producing a crisscross nucleic acid nanostructure, comprising: combining in a reaction mixture (a) a first nanorod comprising a first plug strand and a second plug strand, (b) a second nanorod comprising a third plug strand and a fourth plug strand, wherein the second nanorod is parallel to the first nanorod, (c) a third nanorod comprising a fifth plug strand complementary to and bound to the first plug strand and a sixth plug strand complementary to and bound to the second plug strand, and (d) a fourth nanorod comprising a seventh plug strand complementary to and bound to the third plug strand and an eighth plug strand complementary to and bound to the fourth plug strand, wherein the third nanorod is parallel to the fourth nanorod; and incubating the reaction mixture under conditions that result in assembly of a crisscross nucleic acid nanostructure.
 31. A biomolecule detection method, comprising (a) combining in a reaction mixture (i) a sample comprising a biomolecule; (ii) a nucleic acid strand capable of self-assembling into a nanostructure that comprises stacked parallel strands; (iii) least two crisscross nucleic acid slats of claim 27, wherein the two slats bind to the stacked parallel strands of (ii), and wherein at least two of the slats are linked to a biomolecule binding partner that specifically binds to the biomolecule in the sample; (b) incubating the reaction mixture under conditions that permit binding of the biomolecule binding partners to the biomolecule and assembly of the nanostructure into stacked parallel strands; and (c) incubating the reaction mixture of (b) in the presence of a plurality of crisscross nucleic acid slats of claim 27, wherein the crisscross nucleic acid slats bind to the stacked parallel strands to form a stable three-dimensional nanostructure.
 32. The method of claim 31, further comprising imaging the three-dimensional nanostructure.
 33. A biomolecule detection method, comprising: combining in a reaction mixture (a) a sample comprising a biomolecule and (b) a nucleic acid nanostructure comprising (i) a nucleic acid scaffold strand and nucleic acid staple strands capable of assembling into multiple stacked parallel loops and (ii) two crisscross nucleic acid slats of claim 27 that are programmed to bind to the loops of (i), wherein a biomolecule binding partner that specifically binds to the biomolecule is linked to each of the crisscross nucleic acid slats such that in the presence of the biomolecule the biomolecule binding partners bind to the biomolecule and the nucleic acid nanostructure folds into multiple stacked parallel loops; and incubating the reaction mixture to assemble multiple stacked parallel loops.
 34. The method of claim 33, further comprising combining the reaction mixture with a plurality of crisscross nucleic acid slats of claim 27 to form a three-dimensional nanostructure. 